Publications by authors named "Mikail Dogan"
Objective: Chronic rhinosinusitis (CRS) impacts an estimated 5% to 15% of people worldwide, incurring significant economic healthcare burden. There is a urgent need for the discovery of predictive biomarkers to improve treatment strategies and outcomes for CRS patients.
Study Design: Cohort study of CRS patients and healthy controls using blood samples.
Despite substantial advancements in development of cancer treatments, lack of standardized and physiologically-relevant in vitro testing platforms limit the early screening of anticancer agents. A major barrier is the complex interplay between the tumor microenvironment and immune response. To tackle this, a dynamic-flow based 3D bioprinted multi-scale vascularized breast tumor model, responding to chemo and immunotherapeutics is developed.
View Article and Find Full Text PDFTargeting SARS-CoV-2 infection through CAR-T-like bispecific T cell engagers incorporating ACE2.
Clin Transl Immunology
October 2022
Article Synopsis
- - The study addresses the ongoing health challenges posed by COVID-19 and its variants, which lessen the effectiveness of current vaccines despite advances in antibody treatments and vaccines.
- - Researchers engineered T cells to target the SARS-CoV-2 Spike protein using a bispecific T cell engager called ACE2-Bite, which effectively kills infected cells and neutralizes various virus strains, including Delta and Omicron.
- - The findings indicate that ACE2-Bite could serve as a robust therapeutic strategy against future SARS-CoV-2 variants by harnessing the body’s adaptive immune response.
Engineering Human MAIT Cells with Chimeric Antigen Receptors for Cancer Immunotherapy.
J Immunol
October 2022
Article Synopsis
- Engineering immune cells with chimeric antigen receptors (CARs) shows promise in cancer treatment, particularly using a unique T cell subset known as mucosal-associated invariant T (MAIT) cells.
- In a study, researchers converted MAIT cells into CAR-T cells targeting B cell lymphoma and breast cancer, finding that these CAR-MAIT cells were highly effective against tumors, similar to traditional CD8 CAR-T cells but with lower IFN-γ production.
- Additionally, MAIT cells can be activated by a vitamin B metabolite to enhance their tumor-killing abilities, offering a novel, potentially more accessible approach to immunotherapy.
Immunotherapy has revolutionized cancer treatment with the advent of advanced cell engineering techniques aimed at targeted therapy with reduced systemic toxicity. However, understanding the underlying immune-cancer interactions require development of advanced three-dimensional (3D) models of human tissues. In this study, we fabricated 3D tumor models with increasing complexity to study the cytotoxic responses of CD8T cells, genetically engineered to express mucosal-associated invariant T (MAIT) cell receptors, towards MDA-MB-231 breast cancer cells.
View Article and Find Full Text PDFBackground: Characterization of neutralization antibodies to SARS-CoV-2 infection or vaccination in children and young adults with inflammatory bowel disease (IBD) receiving biologic therapies is crucial.
Methods: We performed a prospective longitudinal cohort study evaluating SARS-CoV-2 spike protein receptor binding domain (S-RBD) IgG positivity along with consistent clinical symptoms in patients with IBD receiving infliximab or vedolizumab. Serum was also obtained following immunization with approved vaccines.
Background: Characterization of neutralization antibodies to SARS-CoV-2 infection or vaccination in children and young adults with inflammatory bowel disease (IBD) receiving biologic therapies is crucial.
Methods: e performed a prospective longitudinal cohort study evaluating SARS-CoV-2 Spike protein receptor binding domain (S-RBD) IgG positivity along with consistent clinical symptoms in patients with IBD receiving infliximab or vedolizumab. Serum was also obtained following immunization with approved vaccines.
Article Synopsis
- - The study focuses on understanding the development of antibody protection against SARS-CoV-2, which is crucial for public health and vaccine creation.
- - Researchers created sensitive tests to detect specific IgG antibodies and neutralization capabilities in individuals infected with SARS-CoV-2, finding high antibody levels in infected patients compared to controls.
- - Results indicate that hospitalized patients have significantly higher antibody and neutralization levels than outpatients, revealing important insights into the antibody response and potential vaccine efficacy.
Article Synopsis
- The study focuses on the development of SARS-CoV-2-specific antibodies during infection, crucial for public health and vaccine design.
- Researchers created sensitive assays that detected high levels of IgG antibodies in all patients with confirmed COVID-19, while negative controls showed no antibodies.
- Results indicated hospitalized patients had significantly higher antibody and neutralization levels than outpatients, revealing insights into the immune response and potential vaccine effectiveness.
Article Synopsis
- The study investigates how genetic variations linked to autoimmune and allergic diseases are often found in regulatory regions called enhancers, many of which are not well understood.
- Researchers used mouse models to analyze a specific enhancer at chromosome 11q13.5 that is functional in certain immune cells (CD4 T cells) and found it plays a crucial role in controlling colitis, a type of inflammatory bowel disease.
- The findings suggest that the enhancer interacts with gene promoters to regulate the expression of a protein called GARP, providing insights into the genetic basis of immune diseases and highlighting GARP as a potential treatment target.
Functional Interrogation of Primary Human T Cells via CRISPR Genetic Editing.
J Immunol
September 2018
Developing precise and efficient gene editing approaches using CRISPR in primary human T cell subsets would provide an effective tool in decoding their functions. Toward this goal, we used lentiviral CRISPR/Cas9 systems to transduce primary human T cells to stably express the Cas9 gene and guide RNAs that targeted either coding or noncoding regions of genes of interest. We showed that multiple genes (, , ) could be simultaneously and stably deleted in naive, memory, effector, or regulatory T cell (Treg) subsets at very high efficiency.
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