Unravelling pollen diet and microbiome influence on honey bee health.

In the last decade, drought has been identified as one of the most relevant climate change factors affecting ecosystem integrity across countries. It can severely affect plant growth in agroecosystems, leading to changes in the trophic potential of nectar and pollen. As a cascade effect, a deficit in the nutritional composition of pollen can weaken pollinators, triggering additional threats to ecosystem stability.

Beekeepers' perceptions toward a new omics tool for monitoring bee health in Europe.

Pressures on honey bee health have substantially increased both colony mortality and beekeepers' costs for hive management across Europe. Although technological advances could offer cost-effective solutions to these challenges, there is little research into the incentives and barriers to technological adoption by beekeepers in Europe. Our study is the first to investigate beekeepers' willingness to adopt the Bee Health Card, a molecular diagnostic tool developed within the PoshBee EU project which can rapidly assess bee health by monitoring molecular changes in bees.

The proteomic content of Varroa destructor gut varies according to the developmental stage of its host.

The nutritional physiology of parasites is often overlooked although it is at the basis of host-parasite interactions. In the case of Varroa destructor, one of the major pests of the Western honey bee Apis mellifera, the nature of molecules and tissues ingested by the parasite is still not completely understood. Here, the V.

Composition and Quality of Honey Bee Feed: The Methodology and Monitoring of Candy Boards.

The nutritional status of a honey bee colony is recognized as a key factor in ensuring a healthy hive. A deficient flow of nectar and pollen in the honey bee colony immediately affects its development, making room for pathogen proliferation and, consequently, for a reduction in the activities and strength of the colony. It is, therefore, urgent for the beekeepers to use more food supplements and/or substitutes in apiary management, allowing them to address colony nutritional imbalances according to the beekeeper's desired results.

Taxonomic identification of Morocco scorpions using MALDI-MS fingerprints of venom proteomes and computational modeling.

The venom of scorpions has been the subject of numerous studies. However, their taxonomic identification is not a simple task, leading to misidentifications. This study aims to provide a practical approach for identifying scorpions based on the venom molecular mass fingerprint (MFP).

Application of a robust MALDI mass spectrometry approach for bee pollen investigation.

Pollen collected by pollinators can be used as a marker of the foraging behavior as well as indicate the botanical species present in each environment. Pollen intake is essential for pollinators' health and survival. During the foraging activity, some pollinators, such as honeybees, manipulate the collected pollen mixing it with salivary secretions and nectar (corbicular pollen) changing the pollen chemical profile.

The Response of the Honey Bee Gut Microbiota to Is Modulated by the Probiotic and the Neonicotinoid Thiamethoxam.

The honey bee is exposed to a variety of biotic and abiotic stressors, such as the highly prevalent microsporidian parasite ( and neonicotinoid insecticides. Both can affect honey bee physiology and microbial gut communities, eventually reducing its lifespan. They can also have a combined effect on the insect's survival.

Molecular diversity assessed by MALDI mass spectrometry of two scorpion species venom from two different locations in Morocco.

Scorpion venom is a cocktail of molecules whose composition is remarkably plastic, controlled by several factors. The Moroccan scorpion fauna is characterized by its richness and high rate of endemism and the venom molecular variability of many species is not yet well characterized. The aim of the present study was to highlight the molecular variability of the venom composition of Androctonus amoreuxi and Buthacus stockmanni (endemic species), both belonging to the Buthidae family, collected from two Moroccan regions, Zagora and Tan-tan.

Honey Bee Larval Hemolymph as a Source of Key Nutrients and Proteins Offers a Promising Medium for Artificial Rearing.

, a major ectoparasite of the Western honey bee , is a widespread pest that damages colonies in the Northern Hemisphere. Throughout their lifecycle, females feed on almost every developmental stage of their host, from the last larval instar to the adult. The parasite is thought to feed on hemolymph and fat body, although its exact diet and nutritional requirements are poorly known.

Parasite and Pesticide Impacts on the Bumblebee () Haemolymph Proteome.

Pesticides pose a potential threat to bee health, especially in combination with other stressors, such as parasites. However, pesticide risk assessment tests pesticides in isolation from other stresses, i.e.

Unraveling the Hemolymph, an Indicator of the Immune Response to Microbial Infections, through Complementary Mass Spectrometry Approaches.

Pollinators, including , are crucial for maintaining biodiversity in ecosystems and for agriculture. Deciphering their immune response under stress conditions is a key issue for protecting these populations. To assess this metric, we analyzed the hemolymph as an indicator of their immune status.

Molecular histoproteomy by MALDI mass spectrometry imaging to uncover markers of the impact of Nosema on Apis mellifera.

Matrix-assisted laser desorption/ionization imaging mass spectrometry (MALDI IMS) is a powerful technology used to investigate the spatio-temporal distribution of a huge number of molecules throughout a body/tissue section. In this paper, we report the use of MALDI IMS to follow the molecular impact of an experimental infection of Apis mellifera with the microsporidia Nosema ceranae. We performed representative molecular mass fingerprints of selected tissues obtained by dissection.

Proteomics of Anatomical Sections of the Gut of -Infected Western Honeybee () Reveals Different Early Responses to spp. Isolates.

Honeybees play an important role in pollinating native plants and agricultural crops and produce valuable hive products. Within the last decade, honeybee colonies have been reported to be in decline, due to both biotic and abiotic stress factors including pathogens and pesticides. This study evaluated the impact of different isolates of spp.

Matrix-assisted laser desorption/ionization mass spectrometry biotyping, an approach for deciphering and assessing the identity of the honeybee pathogen Nosema.

Rationale: The microsporidia are obligate intracellular pathogenic fungi that parasitize a wide range of invertebrate and vertebrate hosts and have important impacts on health, food security and the economy. In this paper, we focus on Nosema ceranae and N. apis, which chronically infect the digestive tract of honeybees, altering their physiology and lifespan.

MALDI-MS Profiling to Address Honey Bee Health Status under Bacterial Challenge through Computational Modeling.

Honey bees play a critical role in the maintenance of plant biodiversity and sustainability of food webs. In the past few decades, bees have been subjected to biotic and abiotic threats causing various colony disorders. Therefore, monitoring solutions to help beekeepers to improve bee health are necessary.

Ultrasound measurement of total body fat in obese adolescents.

Background/aims: To compare body fat (BF) measurements obtained with a new ultrasound method with those assessed by dual-energy X-ray absorptiometry (DEXA) in obese adolescents.

Methods: In 94 adolescents (57 females and 37 males) aged 12-19 years and body mass index (BMI) exceeding 30 kg.m(-2), the z-score BMI for age was 6.

Ultrasound techniques applied to body fat measurement in male and female athletes.

Context: For athletes in disciplines with weight categories, it is important to assess body composition and weight fluctuations.

Objective: To evaluate the accuracy of measuring body fat percentage with a portable ultrasound device possessing high accuracy and reliability versus fan-beam, dual-energy X-ray absorptiometry (DEXA).

Design: Cross-validation study.

Validation of ultrasound techniques applied to body fat measurement. A comparison between ultrasound techniques, air displacement plethysmography and bioelectrical impedance vs. dual-energy X-ray absorptiometry.

Aims: To evaluate the accuracy of body fat percentage (BF%) estimates from a portable, non-traumatizing ultrasound device with high accuracy and reliability compared to dual-energy X-ray absorptiometry (DEXA), the reference technique.

Design: Cross-validation between ultrasound technique (UT), DEXA, air displacement plethysmography (ADP) and bioelectrical impedance (BIA) was developed in the study.

Subjects: A total of 89 healthy subjects (41 women, 48 men), aged 48.

The comparison of rDNA spacer regions of Nosema ceranae isolates from different hosts and locations.

Nosema ceranae is a common microsporidian pathogen, one of two Nosema species that cause "nosema disease" in honeybees, Apis cerana and Apis mellifera. Samples of N. ceranae rDNA from isolates collected in different locations were sequenced and one 5S rRNA was found to be upstream of SSUrRNA.