Biochemical analytes in centrifuged blood samples could be affected by the age of subjects in different time periods and storage temperatures.

Background And Objectives: Collection and providing standard blood samples for examination is critical for all medical laboratories. Currently, for all blood samples taken from the elderly and young people, the same preparation protocol is applied. We hypothesized that the blood taken from older individuals is more vulnerable to preparation conditions compared with the younger ones.

Manufacturing stresses do not differentially impact red blood cells from donors with diabetes.

Background And Objectives: With the rising prevalence of diabetes and expanded blood donor criteria in Canada, individuals with diabetes are increasingly contributing to the blood supply. However, little is known about how routine manufacturing affects red blood cells (RBCs) from this group. This study examined RBC differences in donors with type 1 (T1D) or type 2 diabetes (T2D) following processing to generate red cell concentrates (RCCs).

Cold storage surpasses the impact of biological age and donor characteristics on red blood cell morphology classified by deep machine learning.

Assessment of the morphology of red blood cells (RBCs) can improve clinical benefits following blood transfusion. Deep machine learning surpasses traditional microscopy-based classification methods, offering more accurate and consistent results while reducing time and labor intensity. RBCs from teenage males, teenage females, senior males, and senior females were biologically age-profiled or density-separated into dense/old RBCs (O-RBCs) and less-dense/young (Y-RBCs) throughout hypothermic storage and assessed using image flow cytometry with deep machine learning analysis.

Donor age contributes more to the rheological properties of stored red blood cells than donor sex and biological age distribution.

The quality of stored red cell concentrates (RCCs) has been linked to the biological age distribution of red blood cell (RBC) subpopulations. Teenage male RCCs contain higher proportions of biologically old RBCs, with poorer quality. This study sought to assess the contribution of donor sex and age on the deformability characteristics of RBC subpopulations in stored RCCs.

Hemoglobin-oxygen affinity changes in neonatal blood transfusions: RBC selection insights.

Background: Despite preterm newborns often requiring blood transfusions, we have an incomplete understanding of the impact of adult packed red blood cell (pRBC) transfusions on fetal red blood cell (RBC) oxygen affinity. We investigated the influence of adult pRBC on oxygen binding in fetal RBCs obtained from the umbilical cord of preterm newborns. This included exploring the influence of the biological age of adult pRBCs on the oxygen affinity of fetal blood.

RBC subpopulations in RCCs affected by donor factors.

Understanding red blood cell (RBC) subpopulations is crucial for comprehending donor variability and enhancing transfusion outcomes. This review highlights the significance of RBC subpopulations, focusing on the properties of biologically young and old RBCs and underscores how donor variability impacts transfusion outcomes. The role of senescent RBCs in adverse transfusion reactions and the emerging significance of circulating erythroid cells (CECs) is discussed.

Advancing in vivo assessment of red blood cell transfusions: A call for radiation-free methods in transfusion medicine.

RBC transfusions are a vital clinical therapy to treat anemic patients. The in vivo assessment of red blood cell (RBC) quality post-transfusion is critical to ensuring that the introduction of new RBC products meet established regulatory and clinical quality requirements. Although in vitro quality control testing is routinely performed by blood manufacturers, it is crucial that in vivo tests are performed during the evaluation and regulatory process of new RBC products.

Red cell concentrates from teen male donors contain poor-quality biologically older cells.

Background And Objectives: Donor factors influence the quality characteristics of red cell concentrates (RCCs) and the lesions that develop in these heterogeneous blood products during hypothermic storage. Teen male donors' RCCs contain elevated levels of biologically old red blood cells (RBCs). The aim of this study was to interrogate the quality of units of different donor ages and sexes to unravel the complex interplay between donor characteristics, long-term cold storage and, for the first time, RBC biological age.