Targeting Hepatocellular Carcinoma: Schisandrin A Triggers Mitochondrial Disruption and Ferroptosis.

The main focus of this research was to examine SchA's role in the hepatocellular carcinoma (HCC) development. LO2 and Huh7 cell viability were assessed using the MTT assay. The experiments included flow cytometry, colony formation, transwell, wound healing, and immunofluorescence assays to evaluate apoptosis levels, cells colony-forming ability, ROS levels, invasion and migration ability, and mitochondrial membrane potential.

Enhanced Bioconversion of Cellobiose by Industrial Saccharomyces cerevisiae Used for Cellulose Utilization.

Cellobiose accumulation and the compromised temperature for yeast fermentation are the main limiting factors of enzymatic hydrolysis process during simultaneous saccharification and fermentation (SSF). In this study, genes encoding cellobiose transporter and β-glucosidase were introduced into an industrial Saccharomyces cerevisiae strain, and evolution engineering was carried out to improve the cellobiose utilization of the engineered yeast strain. The evolved strain exhibited significantly higher cellobiose consumption rate (2.

[Effect of fibrous root extract of Coptis chinensis on soil microbes and enzyme activities].

Coptis chinensis is widely used as Chinese medicine herbs and serious soil problems occur after continual cultivation of this medicinal plant. In the preset experiment, fibrous root extract of C. chinensis (REC) was added into soil to study the effect of REC on microbes and enzyme activity in soil.

[Response of ectomycorrhizal fungi to aluminum stress and low potassium soil].

Soil acidification, aluminum (Al3+) toxicity and nutrient deficiency could be some of the most important reasons for the decline and death of forests in tropical and subtropical areas. Ectomycorrhizal fungi for Al3+ resistance and nutrient mobilization are beneficial for preventing forests against Al3+ toxicity and increasing forest productivity. Therefore, Suillus luteus (SI 13), Pisolithus tinctorius (Pt 715) and Suillus subluteus (Ss 00) were grown in liquid culture medium with soil as the sole K source under Al3+ stress to study the fungal growth, organic acid and proton efflux, and potassium (K) unitization.