Structure-function modulation of sodium caseinate: Insights from non-covalent reuterin binding.

This study investigated the effects of varying concentrations of reuterin (3-hydroxypropionaldehyde, 3-HPA) on the structural and functional properties of sodium caseinate (SC). UV spectroscopy and SDS-PAGE analysis demonstrated the formation of SC/3-HPA complexes through non-covalent binding. Fourier transform infrared spectroscopy (FTIR) and fluorescence spectroscopy showed that 3-HPA induced significant conformational changes in SC (P < 0.

Effect of reuterin addition before and after fermentation on yogurt quality.

Reuterin, a nonprotein, broad-spectrum antibacterial substance produced by Limosilactobacillus reuteri through glycerol fermentation, exhibits potential as a biological preservative in dairy products. The work aimed to investigate the effect of adding various concentrations of reuterin (9.125, 18.

[Effects of electron acceptor and light on the abundance of microbial function gene related to soil CH emission].

To explore the effects of different electron acceptors on soil methane emission and responses of soil microorganisms to different light conditions, a strict anaerobic 20-day incubation experiment was conducted with eight treatments: darkness + Fe (DF); darkness + NO (DN); darkness +SO (DS); darkness + distilled water (DCK); light + Fe (LF); light + NO (LN); light +SO (LS); light + distilled water (LCK). The changes of methane concentration in the anaerobic incubation flask and the variation of the abundance of bacteria, archaea, fungi and six soil functional genes were analyzed. Results showed that soil methane emission under NO, SO addition and control (CK) was significantly lower under light conditions than dark, except the Fe treatment.

Functionalized reduced graphene oxide with aptamer macroarray for cancer cell capture and fluorescence detection.

An integrated aptamer macroarray functionalized with reduced graphene oxide (rGO) to specifically capture and sensitively detect cancer cells is reported. The capture for cancer cells is based on effective recognition of the modified rGO surface through the aptamer against epithelial cell adhesion molecule (EpCAM). The rough structure of rGO enhances morphologic interactions between rGO film interface and the cancer cells, while super-hydrophilicity of modified rGO hinders nonspecific cell capture.

Tumor-Penetrating Hierarchically Structured Nanomarker for Imaging-Guided Urinary Monitoring of Cancer.

The capacity to diagnose cancer with the existing endogenous biomarkers remains limited because biomarkers usually act at the tumor site and are thus challenging to be detected directly from body fluids with high sensitivity and specificity, especially in the early stage of tumorigenesis. Here, we demonstrate an exogenous tumor-penetrating nanomarker composed of fluorescent nanoparticles conjugated with specific fluorescein-labeled peptides. The injectable nanomarkers perform four functions: they penetrate the tumor, target sites of cancer, cleave specific peptides by on-target protease, and drop off the labeled peptide into host urine for fluorescent detection.

Fluorometric visualization of mucin 1 glycans on cell surfaces based on rolling-mediated cascade amplification and CdTe quantum dots.

A rolling-mediated cascade (RMC) amplification strategy is described for improved visualization of profiling glycans of mucin 1 (MUC 1) on cell surfaces. CdTe quantum dots (QDs) are used as fluorescent labels. The RMC based amplification allows even distinct glycoforms of MUC1 to be visualized on the surface of MCF-7 cell via an amplified Förster resonance energy transfer (FRET) imaging strategy that works at excitation/emission wavelengths of 345/610 nm.

Metal organic framework MIL-53(Fe) as an efficient artificial oxidase for colorimetric detection of cellular biothiols.

Biothiols play critical roles in many biological processes and their aberrant is related to a variety of syndromes. A simple and reliable colorimetric method is developed in this work for biothiols detection based on an oxidase mimic, a metal organic framework (MOF) MIL-53(Fe), and a peroxidase substrate 3,3',5,5'-tetramethylbenzidine (TMB). In this design, MIL-53(Fe) is utilized to catalyze the conversion of TMB to a blue colored 3,3',5,5'-tetramethylbenzidine diimine, which can be read on a spectrophotometer at 652 nm.

[Effects of calponin-1 gene silencing on the biological behavior of uterine smooth muscle cells].

Objective: To study the effects of calponin-1 expression inhibition on the proliferation , invasiveness, apoptosis and cytoskeleton of uterine smooth muscle cells, and explore the molecular mechanism of calponin-1 in the uterine smooth muscle cells for labor onset.

Methods: siRNA-calponin-1 adenovirus plasmid was constructed and transfected into primarily cultured uterine smooth muscle cells. The proliferation, invasiveness and apoptosis of the cells were determined by MTT assay, matrigel invasion assays and flow cytometry, respectively.