Exploring mechanisms underlying the beneficial effect of whey protein isolate on the biocompatibility of the thermally obtained curdlan-based hydrogel.

This work aimed to compare the biomedical potential of thermally obtained hydrogels consisting of curdlan and a curdlan-whey protein isolate mixture. The purpose of this paper was also to investigate and explain the mechanisms responsible for their different cyto- and biocompatibility. To do this, mechanical and structural properties, wettability, and protein adsorption of biomaterials were analysed and compared.

An advanced spectroscopic and crystallographic study on a synergistic composition of a 1,3,4-thiadiazole derivative and amphotericin B, in model biological liposomal system.

The paper presents the results of the first spectroscopic, microcalorimetric, and crystallographic study analyzing in great detail the strongly synergistic composition containing a selected 1,3,4-thiadiazole derivative: 4-(5-methyl-1,3,4-thiadiazol-2-ylo)benzene-1,3-diol (C1) and the antibiotic amphotericin B (AmB) in model biological DPPC films additionally modified with sterols: cholesterol (Chol) and ergosterol (Erg). The spectroscopic properties of the analyzed composition were studied with the use of spectroscopic methods, including: measurements of electronic fluorescence and absorption spectra with the technique of resonance light scattering (RLS), measurements of stationary fluorescence anisotropy and time-resolved fluorescence lifetimes with the method of single photon counting (TCSPC), circular dichroism spectra (CD), and infrared FTIR spectra. The mentioned methods were further complemented by including microcalorimetric DSC and crystallographic XRD analyses.

Advanced Spectroscopic and Theoretical Study and Assessment of Antimycotic Potential in a Synergistic Composition of a 1,3,4-Thiadiazole Derivative and Amphotericin B.

The paper presents the results of an in-depth spectroscopic, theoretical (quantum chemical), and microbiological study conducted on a promising, synergistic composition of a newly considered 1,3,4-thiadiazole derivative, 1,3,4-thiadiazole: 2,4-dihydroxy--(5-methyl-1,3,4-thiadiazol-2-yl)-benzothioamide (TBTA), and the "gold standard" polyene antibiotic, amphotericin B (AmB). The spectroscopic properties of the system were extensively analyzed with a range of spectroscopic measurement techniques, including electronic fluorescence and absorption spectra, resonance light scattering measurements, circular dichroism spectra, dynamic light scattering, and fluorescence anisotropy, which were further complemented with time-resolved measurements of fluorescence lifetimes performed with the single-photon counting method. The samples were prepared in DMSO solutions and/or PBS buffer to facilitate observation of the monomeric, dimeric, and aggregated forms of the antibiotic previously identified in the literature.

Assessment of the Microbiological Potential and Spectroscopic Properties of New Imino-1,3,4-Thiadiazoles Showing the ESIPT Effect Strongly Enhanced by Aggregation.

There is currently a growing interest in imino derivatives of compounds such as thiadiazoles and other groups of compounds whose extended π-electron systems enhance their photophysical properties. These compounds also show low toxicity and strong antifungal activity, making them effective against fungal pathogens in crops. For the above reasons, in the first part of the paper, the structure of the selected analogs was considered, and detailed spectroscopic analyses were conducted focusing on the excited state intramolecular proton transfer (ESIPT) process taking place in the same.

Understanding the synergistic interaction between a 1,3,4-thiadiazole derivative and amphotericin B using spectroscopic and theoretical studies.

We present a comprehensive spectroscopic study supported by theoretical quantum chemical calculations conducted on a molecular system (4-(5-methyl-1,3,4-thiadiazol-2-yl)benzene-1,3-diol (C1) and the antibiotic Amphotericin B (AmB)) that exhibits highly synergistic properties. We previously reported the strong synergism of this molecular system and now wish to present related stationary measurements of UV-Vis absorption, fluorescence, and fluorescence anisotropy in a polar, aprotic solvent (DMSO and a PBS buffer), followed by time-resolved fluorescence intensity and anisotropy decay studies using different ratios of the selected 1,3,4-thiadiazole derivative to Amphotericin B. Absorption spectra measured for the system revealed discrepancies in terms of the shapes of absorption bands, particularly in PBS.

Synergistic Antifungal Interactions between Antibiotic Amphotericin B and Selected ,,-thiadiazole Derivatives, Determined by Microbiological, Cytochemical, and Molecular Spectroscopic Studies.

In recent years, drug-resistant and multidrug-resistant fungal strains have been more frequently isolated in clinical practice. This phenomenon is responsible for difficulties in the treatment of infections. Therefore, the development of new antifungal drugs is an extremely important challenge.

Impact of Metal Nanoparticles on the Phytochemical and Antioxidative Properties of Rapeseed Oil.

The agricultural uses of nanoparticles continue to be considered as innovative methods that require more in-depth research into their impact on product quality. In our study, we investigated the effects of fertilizers containing metal nanoparticles (silver AgNPs and copper CuNPs) during the plant growth stage of winter rape cultivation, and in most experimental variants, with the exception of the (x2) application of AgNPs, we observed a decrease in the mass of one thousand seeds (MTS). The obtained result was 11.

Spectroscopic characterization and assessment of microbiological potential of 1,3,4-thiadiazole derivative showing ESIPT dual fluorescence enhanced by aggregation effects.

In the presented study, advanced experimental techniques, including electronic absorption and fluorescence spectroscopies [with Resonance Light Scattering (RLS)], measurements of fluorescence lifetimes in the frequency domain, calculations of dipole moment fluctuations, quantum yields, and radiative and non-radiative transfer constants, were used to characterize a selected analogue from the group of 1,3,4-thiadiazole, namely: 4-[5-(naphthalen-1-ylmethyl)-1,3,4-thiadiazol-2-yl]benzene-1,3-diol (NTBD), intrinsically capable to demonstrate enol → keto excited-states intramolecular proton transfer (ESIPT) effects. The results of spectroscopic analyses conducted in solvent media as well as selected mixtures were complemented by considering biological properties of the derivative in question, particularly in terms of its potential microbiological activity. The compound demonstrated a dual fluorescence effect in non-polar solvents, e.

Design, Spectroscopy, and Assessment of Cholinesterase Inhibition and Antimicrobial Activities of Novel Coumarin-Thiadiazole Hybrids.

A novel series of coumarin-thiadiazole hybrids, derived from substituted coumarin-3-carboxylic acids was isolated and fully characterized with the use of a number of spectroscopic techniques and XRD crystallography. Several of the novel compounds showed intensive fluorescence in the visible region, comparable to that of known coumarin-based fluorescence standards. Moreover, the new compounds were tested as potential antineurodegenerative agents via their ability to act as acetylcholinesterase (AChE) and butyrylcholinesterase (BuChE) inhibitors.

Chemometric approach to characterization of the selected grape seed oils based on their fatty acids composition and FTIR spectroscopy.

Addressing the issues arising from the production and trade of low-quality foods necessitates developing new quality control methods. Cooking oils, especially those produced from the grape seeds, are an example of food products that often suffer from questionable quality due to various adulterations and low-quality fruits used for their production. Among many methods allowing for fast and efficient food quality control, the combination of experimental and advanced mathematical approaches seems most reliable.

Novel Coumarin-Thiadiazole Hybrids and Their Cu(II) and Zn(II) Complexes as Potential Antimicrobial Agents and Acetylcholinesterase Inhibitors.

A series of coumarin-thiadiazole hybrids and their corresponding Cu(II) and Zn(II) complexes were synthesized and characterized with the use of spectroscopic techniques. The results obtained indicate that all the coumarin-thiadiazole hybrids act as bidentate chelators of Cu(II) and Zn(II) ions. The complexes isolated differ in their ligand:metal ratio depending on the central metal.

Physical Properties, Spectroscopic, Microscopic, X-ray, and Chemometric Analysis of Starch Films Enriched with Selected Functional Additives.

Biodegradable materials are used in the manufacture of packaging and compostable films and various types of medical products. They have demonstrated a large number of potential practical applications in medicine and particularly in the treatment of various cardiac, vascular, and orthopedic conditions in adults as well in children. In our research, the extrusion-cooking technique was applied to prepare thermoplastic starch (TPS), which was then utilized to obtain environmentally friendly starch-based films.