Fluorogenic Chemical Probe Strategy for Precise Tracking of Mitochondrial Polarity.

Mitochondrial polarity is a critical indicator of numerous pathological and biological processes; thus, the development of fluorescent probes capable of targeting mitochondria and visually monitoring its polarity is of great significance. In this study, fluorescent probes were designed with a N, N-dialkylamino rhodol scaffold as the fluorophore sensitive to polarity environments, in which the alkyl chain length was adjusted rationally to obtain distinct polarity recognition modes. By integrating mitochondria targeting groups, three fluorogenic chemical probes , , and have been obtained, featuring the capability to target mitochondria and monitor its polarity precisely, dynamically and visually.

A system based on deep convolutional neural network improves the detection of early gastric cancer.

Background: Early gastric cancer (EGC) has a high survival rate, but it is difficult to diagnosis. Recently, artificial intelligence (AI) based on deep convolutional neural network (DCNN) has made significant progress in the field of gastroenterology. The purpose of this study was to establish a DCNN assist system to improve the detection of EGC.

Hydrazone derivative bearing coumarin for the relay detection of Cu and HS in an almost neat aqueous solution and bioimaging in lysosomes.

A new morpholine functionalized coumarin-based fluorescent probe 1 was easily synthesized. The probe realized the sequentially detecting of Cu and HS in the HEPES buffer solution (20 mM, pH = 5.0).

Sensitive and selective fluorescent probe for hypochlorite in 100% aqueous solution and its application for lysosome-targetable cell imaging.

A morpholine-functionalized pyrrole-cyanine probe was synthesized via a simple condensation reaction in high yield. This probe exhibits high selectivity toward ClO on fluorescence and UV-vis spectra in neat aqueous solution. The strong green emission of the probe solution was quenched and the yellow color faded immediately upon the addition of ClO.

Quinoline-based hydrazone for colorimetric detection of Co and fluorescence turn-on response of Zn.

A quinoline-based hydrazone, namely, bis((quinolin-8-yl)methylene)carbonohydrazide (1), has been designed and synthesized, which could be used as a dual probe for selective recognition of Co and Zn by monitoring changes in absorption and fluorescence spectral pattern, respectively. The detection limits of 1 for these two ions are 0.21 and 0.

Two new sesquiterpenoid glycosides from the stems of DC.

Two new sesquiterpenoid glycosides as dihydrophaseic acid 4'--[6″--(4″'-hydroxy-3″', 5″'-dimethoxy) benzoyl)]--D-glucopyranoside () and dihydrophaseic acid 4'--[6″--(3″'-methoxy- 4″'-hydroxy) benzoyl)]-β-D-glucopyranoside (), were isolated from the stems of in the study. The compound and showed moderate scavenging activity in DPPH free radical assay with IC values of 241 and 264 μM, respectively.

Triterpenoids from and Their Anti-Inflammatory Effects.

is a medicinal plant of the Euphorbiaceae family, which can produce anti-inflammatory and cancer chemopreventive agents of triterpenoids. The present study reports on the bioactive triterpenoids of this plant. Two new lanostane-type triterpenoids, named (3,4,7,9)-4-methyl-3,7-dihydroxy-7(8→9) -lanost-24(28)-en-8-one () and 24-hydroperoxylanost-7,25-dien-3β-ol (), together with 15 known triterpene derivatives, were isolated from .

Rapid identification and simultaneous analysis of multiple constituents from Rheum tanguticum Maxim. ex Balf. by UPLC/Q-TOF-MS.

Rhubarb contains biologically active compounds such as anthraquinones, anthrones, stilbenes and tannins. A rapid and efficient UPLC/Q-TOF-MS/MS method was developed and applied towards identifying the constituents of Rheum tanguticum Maxim. ex Balf.

Fourier transform mid-infrared spectroscopy (FT-MIR) combined with chemometrics for quantitative analysis of dextrin in Danshen (Salvia miltiorrhiza) granule.

The granule of Chinese medicine (GCM) is prepared by water-soluble extract of single yinpian (WESY) of herbal medicine, and used as a drug ingredient for clinical formulation. The WESY content or corresponding yinpian amount is the most important parameter in evaluating the quality of GCM. Low WESY content reflects poor GCM.

A new cassane diterpenoid from the seeds of Caesalpinia decapetala.

Phytochemical investigation on the seeds of Caesalpinia decapetala led to the isolation of a new cassane diterpenoid with an unusual O bridge between C-19 and C-20, named phanginin Q (1), together with three known cassane diterpenoids, caesaljapin (2), caesaldekarin A (3), and caesaldekarin B (4). The structure of the new compound was elucidated by spectroscopic methods, including (1)H NMR, (13)C NMR, HSQC, (1)H - (1)H COSY, HMBC, NOESY, and HR-ESI-MS.

[Effects of anti-osteopontin antibody on expression of MMP-2 and TGF-beta1 in hepatic alveolar hydatid tissue of gerbil].

Objective: To observe the effect of anti-osteopontin antibody on the level of matrix metalloproteinase (MMP-2) and TGF-beta1 in gerbils infected with Echinococcus multilocularis.

Methods: One hundred and eighty gerbils were infected with Echinococcus protoscoleces (approximately 400 for each gerbil) by abdominal opening inoculation in liver. The gerbils were randomly divided into three groups: anti-osteopontin antibody experiment group (group A), rabbit serum injection group (group B), and model group (group C).

[Effect of anti-osteopontin antibody on expression of IL-2 and IL-5 in hepatic alveolar hydatid tissue of gerbil].

Objective: To observe the effect of anti-osteopontin antibody on the level of IL-2 and IL-5 in the liver of gerbil infected with Echinococcus multilocularis (Em).

Methods: 180 gerbils were infected with echinococcus protoscoleces (approximately 400 for each gerbil) by abdominal opening inoculation in liver. The gerbils were randomly divided into three groups with 60 each: experiment group (group A, with anti-osteopontin antibody interference), control group (group B, with normal rabbit serum injection) and model group (group C, with no interference).

Three novel sterols isolated from Selaginella tamariscina with antiproliferative activity in leukemia cells.

Three new sterols, 3beta,16alpha-dihydroxy-5alpha,17beta-cholestan-21-carboxylic acid (1), 3beta-acetoxy-16alpha-hydroxy-5alpha,17beta-cholestan-21-carboxylic acid (2) and 3beta-(3-hydroxybutyroxy)-16alpha-hydroxy-5alpha,17beta-cholestan-21-carboxylic acid (3) were isolated from Selaginella tamariscina (Beauv.) Spring (Selaginellaceae). Their structures were elucidated based on NMR analyses.