Myosin XI is required for boron transport under boron limitation via maintenance of endocytosis and polar localization of the boric acid channel AtNIP5;1.

Myosin XI plays a major role in cytoplasmic streaming and is essential for intracellular transport. Here, we investigated the physiological roles of myosin XI in nutrient transport using double (2ko) and triple (3ko) myosin XI knockout mutants of Arabidopsis thaliana. The results revealed that the mutants exhibited more severe boron deficiency phenotypes under boron-limiting conditions, and the boron concentration in the aerial parts of mutant plants was lower than that in the wild-type.

The polar-localized borate exporter BOR1 facilitates boron transport in tapetal cells to the developing pollen grains.

Boron is an essential micronutrient required for plant cell wall integrity, as it is necessary for crosslinking the pectic polysaccharide rhamnogalacturonan II. Reproductive organs require a greater amount of boron for development and growth compared with vegetative organs. However, the mechanism by which plants distribute boron to specific organs is not fully understood.

Localization of the MTP4 transporter to trans-Golgi network in pollen tubes of Arabidopsis thaliana.

Zinc (Zn) is an essential element for plants. Numerous proteins in different cellular compartments require Zn for their structure and function. Zn can be toxic when it accumulates in high levels in the cytoplasm.

Casparian strips prevent apoplastic diffusion of boric acid into root steles for excess B tolerance.

Casparian strips are ring-like structures consisting of lignin, sealing the apoplastic space between endodermal cells. They are thought to have important functions in controlling radial transport of nutrients and toxic elements in roots. However, mutants with a defective Casparian strip structure have been found to maintain nutrient homeostasis in ranges supportive of growth under standard laboratory conditions.

ANTH domain-containing proteins are required for the pollen tube plasma membrane integrity via recycling ANXUR kinases.

During plant reproduction, sperm cells are delivered to ovules through growing pollen tubes. This process involves tip-localized receptor kinases regulating integrity and/or guidance of pollen tubes, whose localizations must be strictly regulated. However, the molecular basis for tip-localization of these molecules remains largely elusive.