Removal of meropenem from hospital wastewater using biochar derived from grape stalks.

This study focuses on synthesizing biochar from grape stalks and its application for removing the antibiotic meropenem (MRP) from aqueous solutions. The raw biochar was chemically activated with NaOH under mild conditions and thoroughly characterized to elucidate its adsorption mechanisms. Preliminary tests confirmed the need for activation to enhance the adsorption efficiency.

Using Digital PCR to Unravel the Occurrence of Piroplasmids, spp., and spp. in Wild Animals from Brazil.

Piroplasmids ( spp., spp., spp.

Effects of Ladder-Climbing Exercise on Mammary Cancer: Data from a Chemically Induced Rat Model.

Breast cancer remains a significant global health issue, affecting both humans and companion animals, particularly female dogs and cats, where mammary tumors are among the most common cancers. Strategies to minimize the impact of this disease on patients, pet owners, and veterinary medicine are essential. This study analyses the effects of resistance training on the development of chemically induced mammary cancer in female Wistar rats, divided into four groups: sedentary control (CTR), sedentary induced (CTR+-methyl--nitrosourea (MNU)), exercised control (EX), and exercised induced (EX+MNU).

Effect of oxytocin and cloprostenol on the seminal parameters of red brocket deer () during the electroejaculation procedure.

Successful implementation of conservation programs for endangered species requires biological material for use in reproductive biotechnologies. This enhances reproductive efficiency and helps increase the populations of critically endangered species. One way to facilitate the exchange of genetics between captive and free-ranging animals is through the creation of cryogenic banks that store cryopreserved gametes.

Transport Media for Live Skin Tissue from Gray-Brocket Deer ().

Sampling skin fragments has been an important strategy for genetic studies and conservation, aiding in the preservation of genetic diversity in Neotropical deer and other wild species. From the moment of collection in the field, transport media must ensure tissue viability by providing the necessary nutrients until laboratory processing for culture or cryopreservation. This study aimed to evaluate the effects of temperature and storage duration on tissue viability and cell growth using two types of skin transport media: Dulbecco's modified Eagle medium (DMEM) supplemented with 15% fetal bovine serum and 0.

Immune modulation in solid tumors: a phase 1b study of RO6870810 (BET inhibitor) and atezolizumab (PD-L1 inhibitor).

Purpose: Bromodomain and extra-terminal domain (BET) inhibitors (BETi) have demonstrated epigenetic modulation capabilities, specifically in transcriptional repression of oncogenic pathways. Preclinical assays suggest that BETi potentially attenuates the PD1/PD-L1 immune checkpoint axis, supporting its combination with immunomodulatory agents.

Patients And Methods: A Phase 1b clinical trial was conducted to elucidate the pharmacokinetic and pharmacodynamic profiles of the BET inhibitor RO6870810 as monotherapy and in combination with the PD-L1 antagonist atezolizumab in patients with advanced ovarian carcinomas and triple-negative breast cancer (TNBC).

Visualizing and analyzing 3D biomolecular structures using Mol* at RCSB.org: Influenza A H5N1 virus proteome case study.

The easiest and often most useful way to work with experimentally determined or computationally predicted structures of biomolecules is by viewing their three-dimensional (3D) shapes using a molecular visualization tool. Mol* was collaboratively developed by RCSB Protein Data Bank (RCSB PDB, RCSB.org) and Protein Data Bank in Europe (PDBe, PDBe.

Multi-scale structural similarity embedding search across entire proteomes.

The rapid expansion of three-dimensional (3D) biomolecular structure information, driven by breakthroughs in artificial intelligence/deep learning (AI/DL)-based structure predictions, has created an urgent need for scalable and efficient structure similarity search methods. Traditional alignment-based approaches, such as structural superposition tools, are computationally expensive and challenging to scale with the vast number of available macromolecular structures. Herein, we present a scalable structure similarity search strategy designed to navigate extensive repositories of experimentally determined structures and computed structure models predicted using AI/DL methods.

The Impact of a Western Diet and Resistance Training in a Rat Model of Mammary Cancer.

This study aimed to investigate the impact of a Western diet and resistance training on cardiac remodeling in a rat model of chemically induced mammary cancer. Fifty-six female Wistar rats were randomly assigned to one of eight experimental groups, evaluating the impact of Western and standard diets, exercise and sedentarism, and the induction of mammary cancer. Mammary cancer was induced via the intraperitoneal administration of -methyl--nitrosourea (MNU) (50 mg/kg) at seven weeks of age.

PhageDPO: A machine-learning based computational framework for identifying phage depolymerases.

Bacteriophages (phages) are the most predominant and genetically diverse biological entities on Earth. Phages are viruses that infect bacteria and encode numerous proteins with potential biotechnological application. However, most phage-encoded proteins remain functionally uncharacterized.

rcsb-api: Python Toolkit for Streamlining Access to RCSB Protein Data Bank APIs.

The Protein Data Bank (PDB) was founded in 1971 as the first open-access digital data resource in biology to serve as the single global archive for three-dimensional (3D) macromolecular structure data. Current PDB holdings exceed 230,000 experimentally determined structures of proteins, nucleic acids, viruses, and macromolecular machines. The RCSB Protein Data Bank RCSB.

Age, cancer, and the dual burden of cancer and doxorubicin in skeletal muscle wasting in female rats: which one to blame?

Sarcopenia and cancer cachexia are two life-threatening conditions often misdiagnosed. The skeletal muscle is one of the organs most adversely affected by these conditions, culminating in poor quality of life and premature mortality. In addition, it has been suggested that chemotherapeutic agents exacerbate cancer cachexia, as is the case of doxorubicin.

Mammary Cancer Models: An Overview from the Past to the Future.

Breast cancer research heavily relies on diverse model systems to comprehend disease progression, develop novel diagnostics, and evaluate new therapeutic strategies. This review offers a comprehensive overview of mammary cancer models, covering both ex vivo and in vivo approaches. We delve into established techniques, such as cell culture and explore cutting-edge advancements, like tumor-on-a-chip and bioprinting.

Updated resources for exploring experimentally-determined PDB structures and Computed Structure Models at the RCSB Protein Data Bank.

The Research Collaboratory for Structural Bioinformatics Protein Data Bank (RCSB PDB, RCSB.org), the US Worldwide Protein Data Bank (wwPDB, wwPDB.org) data center for the global PDB archive, provides access to the PDB data via its RCSB.

Interplay Between Western Diet and Mammary Cancer: Data from a Chemically-induced Model in Wistar Rats.

Background/aim: This study aimed to investigate the influence of Western diet on mammary cancer in Wistar female rats, focusing on systemic responses and tumor development.

Materials And Methods: Twenty-eight Wistar female rats were acclimatized and divided into four experimental groups (n=7 each): Western diet (WD), Western diet with N-methyl-N-nitrosourea (MNU) administration (WD+MNU), standard diet (CTR), and standard diet with MNU administration (CTR+MNU). MNU was administered intraperitoneally at 50 mg/kg at seven weeks of age to induce mammary cancer.

Realistic Aspects of Cardiac Ultrasound in Rats: Practical Tips for Improved Examination.

Echocardiography is a reliable and non-invasive method for assessing cardiac structure and function in both clinical and experimental settings, offering valuable insights into disease progression and treatment efficacy. The successful application of echocardiography in murine models of disease has enabled the evaluation of disease severity, drug testing, and continuous monitoring of cardiac function in these animals. However, there is insufficient standardization of echocardiographic measurements for smaller animals.

Polymelia and concurrent intestinal duplication and cloacal atresia in a broiler chick: case report and literature review.

Congenital malformations are a highly diverse group of conditions reported in both humans and animals, characterized by defects in morphogenesis observed at birth. Although most cases are idiopathic, genetic and environmental factors may be involved. The frequency of such conditions varies with species, geographic regions, and the specific malformation involved.

Long-term effects of the chronic administration of doxorubicin on aged skeletal muscle: An exploratory study in mice.

The widely used chemotherapeutic drug doxorubicin (DOX) has been associated with adverse effects on the skeletal muscle, which can persist for years after the end of the treatment. These adverse effects may be exacerbated in older patients, whose skeletal muscle might already be impaired by aging. Nonetheless, the mediators responsible for DOX-induced myotoxicity are still largely unidentified, particularly the ones involved in the long-term effects that negatively affect the quality of life of the patients.

Exposing illegal hunting and wildlife depletion in the world's largest tropical country through social media data.

Globally, illegal sport hunting can threaten prey populations when unregulated. Due to its covert nature, illegal sport hunting poses challenges for data collection, hindering efforts to understand the full extent of its impacts. We gathered social media data to analyze patterns of illegal sport hunting and wildlife depletion across Brazil.