Study on the spectrum-effect correlation of phytoestrogenic activity of total flavonoids from Cynomorii Herba.

Ethnopharmacological Relevance: Cynomorii Herba (CH), a traditional Chinese medicine, contains total flavonoids with potential phytoestrogenic activity, but the specific active components and mechanisms of action remain unclear.

Aim Of The Study: To investigate the active components and mechanisms responsible for the phytoestrogenic activity of Cynomorii Herba Total Flavonoids (CHTF).

Materials And Methods: A comprehensive fingerprint analysis of CHTF from 12 different regions was conducted using ultra-performance liquid chromatography-mass spectrometry (UPLC-MS) technology.

TGFβ1 induces hypertrophic change and expression of angiogenic factors in human chondrocytes.

The transforming growth factor β1 (TGFβ1) plays an important role in cartilage development. However, whether TGFβ1 stimulates chondrocyte proliferation and cartilage regeneration in osteoarthritis (OA) remains elusive, especially in the context of different treatment and tissue environments. In the present study, we investigated the role of TGFβ1 in human chondrocyte culture , focusing on the morphological change of chondrocytes and the expression of angiogenic factors upon TGFβ1 stimulation.

Cellular compatibility of magnetic nanocomposites.

Objective: To study the cellular compatibility of the magnetic nanocomposite (n-HA/PLLA/Fe2O3) and to discuss the biological behaviors of cells including surface adhesion, proliferation, and expression. The present work provides an experimental basis for medical application.

Method: Rat osteoblasts (OB) were co-cultured with the magnetic nanocomposite.

Extracellular matrix production in vitro in cartilage tissue engineering.

Cartilage tissue engineering is arising as a technique for the repair of cartilage lesions in clinical applications. However, fibrocartilage formation weakened the mechanical functions of the articular, which compromises the clinical outcomes. Due to the low proliferation ability, dedifferentiation property and low production of cartilage-specific extracellular matrix (ECM) of the chondrocytes, the cartilage synthesis in vitro has been one of the major limitations for obtaining high-quality engineered cartilage constructs.

The study on biocompatibility of porous nHA/PLGA composite scaffolds for tissue engineering with rabbit chondrocytes in vitro.

Objective: To examine the biocompatibility of a novel nanohydroxyapatite/poly[lactic-co-glycolic acid] (nHA/PLGA) composite and evaluate its feasibility as a scaffold for cartilage tissue engineering.

Methods: Chondrocytes of fetal rabbit were cultured with nHA/PLGA scaffold in vitro and the cell viability was assessed by MTT assay first. Cells adhering to nHA/PLGA scaffold were then observed by inverted microscope and scanning electron microscope (SEM).

Molecular cloning and functional analysis of polymeric immunoglobulin receptor gene in orange-spotted grouper (Epinephelus coioides).

As one of the most important mucosal effectors, polymeric immunoglobulin receptor (pIgR) mediates the transcytosis of polymeric immunoglobulins (pIgs) to protect the organisms. In this study, a full-length cDNA of pIgR was isolated from orange-spotted grouper (Epinephelus coioides), and the sequence analysis of deduced protein revealed the presence of only two Ig-like domains (ILDs), and the absence of the conserved Ig-binding site and complementary determining region (CDR). The grouper pIgR mRNA was detected in almost all the peripheral tissues examined, especially the mucosal tissues by RT-PCR.

Interleukin-1beta gene in orange-spotted grouper, Epinephelus coioides: molecular cloning, expression, biological activities and signal transduction.

IL-1beta, a key mediator of inflammation, orchestrates a variety of immune responses by initiating gene expression. Herein, we have cloned and sequenced the IL-1beta in orange-spotted grouper (Epinephelus coioides), produced soluble mature recombinant IL-1beta in Escherichia coli, and characterized its biological properties and downstream signal transduction. The grouper IL-1beta cDNA was 1364bp in length, containing an open reading frame of 765bp.