Cancer incidence associations with drinking water arsenic levels and disinfection methods in Maine, USA.

Maine is a largely rural state where nearly half of the population uses drinking water from private wells. Arsenic (As) is present in some Maine groundwater, has been linked to cancer, and a lack of testing and treatment may expose people with private wells to elevated As levels. Disinfection byproducts (DBPs) include known and suspected carcinogens that form when chlorine or chloramines are added to water.

Safe Circular Food Systems: A Transdisciplinary Approach to Identify Emergent Risks in Food Waste Nutrient Cycling.

With growing awareness of the environmental, economic, and social costs associated with food waste, there is a concerted effort on multiple scales to recover the nutrient value of discarded food. These developments are positive, but the rapid movement toward alternatives and the complexity of solving problems located at the intersection of economic, social, and environmental systems also have the potential to produce unanticipated risks. This paper draws upon long-term stakeholder-engaged research throughout New England, with a focus on Maine, to develop a transdisciplinary, systems-based model of the potential social, economic, and environmental risks of food waste nutrient cycling.

Management of per- and polyfluoroalkyl substances (PFAS)-laden wastewater sludge in Maine: Perspectives on a wicked problem.

This article discusses the challenges and potential solutions for managing wastewater sludge that contains per- and polyfluoroalkyl substances (PFAS), using the experience in Maine as a guide toward addressing the issue nationally. Traditional wastewater treatment, designed to remove excess organic waste and nutrients, does not eliminate persistent toxic pollutants like PFAS, instead partitioning the chemicals between discharged effluent and the remaining solids in sludge. PFAS chemistry, the molecular size, the alkyl chain length, fluorine saturation, the charge of the head group, and the composition of the surrounding matrix influence PFAS partitioning between soil and water.

Linking drivers of plant per- and polyfluoroalkyl substance (PFAS) uptake to agricultural land management decisions.

Widespread contamination of the per- and polyfluoroalkyl substance (PFAS) in agricultural areas is largely attributed to the application of sewage sludge in which the PFAS can be concentrated. This creates a pathway for these contaminants to enter the food chain and, by extension, causes human health and economic concerns. One barrier to managing land with PFAS contamination is the variation in reported plant uptake levels across studies.

Evidence of compost contamination with per- and polyfluoroalkyl substances (PFAS) from "compostable" food serviceware.

Per- and polyfluoroalkyl substances (PFAS) have been used to waterproof and greaseproof food serviceware for decades. Health concerns about these compounds have drawn attention to the potential for contamination of the food system. Finished compost (n = 3) made from manure and food serviceware labeled "compostable" generated at a large fair was found to contain 12 or 13 of the 28 PFAS compounds sampled for, in concentrations ranging from 1.

Water temperature and disease alters bacterial diversity and cultivability from American lobster () shells.

The American lobster, , is an economically valuable and ecologically important crustacean along the North Atlantic coast of North America. Populations in southern locations have declined in recent decades due to increasing ocean temperatures and disease, and these circumstances are progressing northward. We monitored 57 adult female lobsters, healthy and shell diseased, under three seasonal temperature cycles for a year, to track shell bacterial communities using culturing and 16S rRNA gene sequencing, progression of epizootic shell disease using visual assessment, and antimicrobial activity of hemolymph.

Many Questions Remain Unanswered About the Role of Microbial Transmission in Epizootic Shell Disease in American Lobsters ().

Despite decades of research on lobster species' biology, ecology, and microbiology, there are still unresolved questions about the microbial communities which associate in or on lobsters under healthy or diseased states, microbial acquisition, as well as microbial transmission between lobsters and between lobsters and their environment. There is an untapped opportunity for metagenomics, metatranscriptomics, and metabolomics to be added to the existing wealth of knowledge to more precisely track disease transmission, etiology, and host-microbe dynamics. Moreover, we need to gain this knowledge of wild lobster microbiomes before climate change alters environmental and host-microbial communities more than it likely already has, throwing a socioeconomically critical industry into disarray.

Composition and contamination of source separated food waste from different sources and regulatory environments.

Food waste recycling is needed to create a more sustainable, circular food system; however, the process must be carefully managed to avoid the introduction and build-up of contaminants. We collected and screened source-separated food waste for five classes of contaminants (physical contaminants, heavy metals, halogenated organics, pathogens and antibiotic resistance genes) from two regulatory environments (voluntary vs mandated food separation) to quantify contamination. Physical contamination was frequently found; 57% of samples contained non-compostable waste.

Effect of air nanobubbles on oxygen transfer, oxygen uptake, and diversity of aerobic microbial consortium in activated sludge reactors.

Nanobubbles have the potential to curtail the loss of oxygen during activated sludge aeration due to their extensive surface areas and lack of buoyance in solution. In this study, nanobubble aeration was explored as a novel approach to enhance aerobic activated sludge treatment and benchmarked against coarse bubble aeration at the lab scale. Nanobubble aerated activated sludge reactors achieved greater dissolved oxygen levels at faster rates.

Bacterial transfer from nematodes to the invasive ant and the potential for colony mortality in coastal Maine.

The necromenic nematode has been frequently found in nests of the invasive European ant in coastal Maine, United States, and may contribute to ant mortality and collapse of colonies by transferring environmental bacteria. and several other bacterial species were found in the digestive tracts of nematodes harvested from collapsed ant colonies. , , and were collected from the hemolymph of nematode-infected wax moth () larvae.

A review of chemical and microbial contamination in food: What are the threats to a circular food system?

A circular food system is one in which food waste is processed to recover plant nutrients and returned to the soil to enable the production of more food, rather than being diverted to landfill or incineration. The approach may be used to reduce energy and water use in food production and contribute to the sustainability of the system. Anaerobic digestion and composting are common food waste treatment technologies used to stabilize waste and produce residual materials that can replenish the soil, thus contributing to a circular food system.

Snowmelt periods as hot moments for soil N dynamics: a case study in Maine, USA.

The vernal transition represents the seasonal transition to spring, occurring as temperatures rise at the end of winter. With rapid snowmelt, microbial community turnover, and accelerated nutrient cycling, this is a critical but relatively under-studied period of ecosystem function. We conducted a study over two consecutive winters (2015-2016) at the Bear Brook Watershed in Maine to examine how changing winter conditions (warming winters, reduced snow accumulation) altered soil nitrogen availability and stream N export during winter and the vernal transition, and how these patterns were influenced by ecosystem N status (N-enriched vs.

Microbial metabolisms in a 2.5-km-deep ecosystem created by hydraulic fracturing in shales.

Hydraulic fracturing is the industry standard for extracting hydrocarbons from shale formations. Attention has been paid to the economic benefits and environmental impacts of this process, yet the biogeochemical changes induced in the deep subsurface are poorly understood. Recent single-gene investigations revealed that halotolerant microbial communities were enriched after hydraulic fracturing.

Temporal changes in microbial ecology and geochemistry in produced water from hydraulically fractured Marcellus shale gas wells.

Microorganisms play several important roles in unconventional gas recovery, from biodegradation of hydrocarbons to souring of wells and corrosion of equipment. During and after the hydraulic fracturing process, microorganisms are subjected to harsh physicochemical conditions within the kilometer-deep hydrocarbon-bearing shale, including high pressures, elevated temperatures, exposure to chemical additives and biocides, and brine-level salinities. A portion of the injected fluid returns to the surface and may be reused in other fracturing operations, a process that can enrich for certain taxa.

Correlations between arsenic in Maine groundwater and microbial populations as determined by fluorescence in situ hybridization.

Arsenic is known to cause serious health effects when consumed in drinking water. In the state of Maine, approximately half of the population relies on private groundwater wells for their drinking water. Of those wells, as many as 13% may contain arsenic levels above the current EPA maximum contaminant level of 10 microgl(-1).

Polybrominated diphenyl ethers in fish and wastewater samples from an area of the Penobscot River in central Maine.

Polybrominated diphenyl ethers (PBDEs) are one class of flame retardants commonly used in textiles, foams and plastics. They are similar in behavior to the well-studied polychlorinated biphenyls and growing evidence suggests they are widespread global environmental pollutants that are capable of bioaccumulation. Fish tissue samples were collected from sites along the Penobscot River in central Maine.

Uptake of mercury by thiol-grafted chitosan gel beads.

This study describes the synthesis and characterization of thiol-grafted chitosan beads for use as mercury (Hg) adsorbents. Chitosan flakes were dissolved and formed into spherical beads using a phase inversion technique, then crosslinked to improve their porosity and chemical stability. Cysteine was grafted onto the beads in order to improve the adsorption affinity of Hg to the beads.

Kinetics of cadmium uptake by chitosan-based crab shells.

Crushed crab shells were chemically treated to transform the chitin present into chitosan. Three particle sizes with average diameters of 0.65, 1.