Publications by authors named "Jayasankar Subramanian"

Strawberries face marketing challenges due to their short post-harvest shelf-life, largely impacted by shrivelling, weight loss, fungal decay, and mechanical damage. Neem oil (NO) is known for its shelf-life extension benefits; however, encapsulation is needed to maintain its efficacy. This study aimed to stabilize and encapsulate NO in a polymeric and lipid material to preserve the quality of strawberries stored at 4 ± 1 °C, 80 ± 2% RH for seven days.

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Stone fruits, also known as drupes, include apricots, peaches, plums, cherries, and nectarines that have high global demand due to their nutritional benefits and palatable characteristics. Being soft fruits, they are susceptible to various postharvest issues, reducing their shelf life, with postharvest loss reaching 15%-50%. Among various postharvest management techniques, edible coating is emerging as a popular method due to its positive effects on the quality, physiochemical, phytochemical, and organoleptic characteristics of these fruits.

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Little is known regarding the genes, compounds and physiological alternations that take place upon infection of black knot disease. This research aimed to unravel the genetic mechanism responsible for the resistance of Japanese plum ( L.) trees against black knot ( Schwein.

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Article Synopsis
  • Starch, especially with high amylose content, is effective for encapsulating hydrophobic compounds like essential oils, making it valuable in food, pharmaceutical, and cosmetic industries.
  • Pea starch, which has a high amylose content, is not usable directly in food but can be converted into nanoparticles to encapsulate neem oil, a natural antimicrobial agent.
  • An optimized synthesis method resulted in oil-encapsulated starch nanoparticles (OESNP) that showed high encapsulation efficiency, small particle size, and sustained oil release, suggesting potential applications in agriculture for delivering natural pesticides using industrial pea starch waste.
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This study was conducted for the comparative analysis of antioxidant activity and untargeted metabolomics of dark- and light-colored sour cherry cultivars grown in Canada. Based on our previous study, we selected four cultivars-'Heimann R', 'Gorsemska', V70142, and 'Montmorency'-to determine the untargeted metabolites and their role in antioxidant activities. A total of 473 metabolites were identified from four sour cherry genotypes using UPLC-ToF-MS.

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Black knot (BK) is a deadly disease of European () and Japanese () plums caused by the hemibiotrophic fungus Generally, phytopathogens hamper the balance of primary defense phytohormones, such as salicylic acid (SA)-jasmonic acid (JA) balance, for disease progression. Thus, we quantified the important phytohormone titers in tissues of susceptible and resistant genotypes belonging to European and Japanese plums at five different time points. Our previous results suggested that auxin-cytokinins interplay driven by appeared to be vital in disease progression by hampering the plant defense system.

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Black Knot (BK) is a deadly disease of European () and Japanese () plums caused by the hemibiotrophic fungus . After infection, the appearance of warty black knots indicates a phytohormonal imbalance in infected tissues. Based on this hypothesis, we quantified phytohormones such as indole-3-acetic acid, tryptophan, indoleamines (N-acetylserotonin, serotonin, and melatonin), and cytokinins (zeatin, 6-benzyladenine, and 2-isopentenyladenine) in temporally collected tissues of susceptible and resistant genotypes belonging to European and Japanese plums during of BK progression.

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Article Synopsis
  • Camelina is a versatile oilseed crop that has been genetically engineered to enhance its yield, fatty acid composition, protein profiles, and resistance to drought.
  • Concerns arise regarding the risk of transgenic traits transferring to non-transgenic camelina and wild relatives, prompting the need for effective bioconfinement strategies to prevent pollen-mediated gene flow (PMGF).
  • The study successfully implemented a gene from peach to promote cleistogamy (closed flowers) in transgenic camelina, which significantly reduced PMGF in field trials, making it a promising bioconfinement approach for this and potentially other crop species.
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Fruits and vegetables are an integral part of our diet attributed to their appealing taste, flavor, and health-promoting characteristics. However, due to their high-water activity, they are susceptible to microbial spoilage and diseases at any step in the food supply chain, from pre-harvest treatment to post-harvest storage and transportation. As a result, food researchers and engineers are developing innovative technologies that can be used to reduce the loss of fruits and vegetables on-farm and during postharvest processing.

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Carrot processing industries produce 25-30% of waste in the form of carrot rejects, peels, and pomace which contain a large amount of high-value bioactive components. Green extraction of the bioactive components from carrot rejects with green solvents using closed-vessel energy-intensive microwave-assisted extraction was the objective of this work. In this work, three experimental studies were implemented.

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Anthocyanins, a major class of flavonoids, are important pigments of grape berries. Despite the recent discovery of the genetic cause underlying the loss of color, the metabolomic and molecular responses are unknown. Anthocyanin quantification among diverse berry color muscadines suggests that all genotypes could produce adequate anthocyanin quantities, irrespective of berry color.

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Acute myeloid leukemia (AML) is an aggressive blood cancer with limited chemotherapy options and negative patient outcomes. Investigations with bioactive compounds from dietary sources against cancer have increased in the recent years, which highlight the need for novel therapeutic approaches and new anti-leukemic agents possessing higher efficacy and selectivity for AML cells and fewer negative side effects. Bioactive compounds demonstrated the ability to induce cell cycle blockage and apoptosis or autophagy in cancer cells, as well as inhibition of proliferation/migration and tumor progression, etc.

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'Honeycrisp' ( Borkh.), a premium applecultivar, is highly susceptible to bitter pit and decline in quality during long-term storage. In order to enhance the quality, an aqueous composition containing hexanal was applied as a preharvest spray.

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Apples (  Borkh) are prone to preharvest fruit drop, which is more pronounced in 'Honeycrisp'. Hexanal is known to improve fruit retention in several economically important crops. The effects of hexanal on the fruit retention of 'Honeycrisp' apples were assessed using physiological, biochemical, and transcriptomic approaches.

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Plums are affected by a cancerous disease called "black knot disease" caused by the fungus . It affects both Japanese () and European () plums equally. To understand the spread of the disease, histological analysis was performed in two different European plum cultivars (susceptible and tolerant).

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Fruit development is orchestrated by a complex network of interactions between hormone signaling pathways. The phytohormone gibberellin (GA) is known to regulate a diverse range of developmental processes; however, the mechanisms of GA action in perennial fruit species are yet to be elucidated. In the current study, a GA signaling gene PslSLY1, encoding a putative F-box protein that belongs to the SLY1 (SLEEPY1)/GID2 (gibberellin-insensitive dwarf2) gene family, was isolated from Japanese plum (Prunus salicina).

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Hexanal, a C-6 aldehyde has been implicated to have antimicrobial properties. Hence, this study was conducted to determine the antifungal activities of hexanal vapor against major postharvest pathogens of banana ., and .

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Raspberry (Rubus spp.) is an economically important crop with a restricted growing season and very limited fruit shelf-life due to its extreme tenderness. In order to prolong its shelf life, an aqueous composition containing hexanal as the key active ingredient (HC) was applied as a preharvest spray during fruit development.

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Phosphatidylinositol 3-kinase (PI3K) is a key enzyme that phosphorylates phosphatidylinositol at 3'-hydroxyl position of the inositol head group initiating the generation of several phosphorylated phosphatidylinositols, collectively referred to as phosphoinositides. The function of PI3K in plant senescence and ethylene signal transduction process was studied by expression of PI3K in transgenic , and delineating its effect on flower senescence. Detached flowers of transgenic tobacco plants with overexpressed - (OX) displayed accelerated senescence and reduced longevity, when compared to the flowers of wild type plants.

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Fruit growth depends on highly coordinated hormonal activities. The phytohormone gibberellin (GA) promotes growth by triggering degradation of the growth-repressing DELLA proteins; however, the extent to which such proteins contribute to GA-mediated fruit development remains to be clarified. Three new plum genes encoding DELLA proteins, PslGAI, PslRGL and PslRGA were isolated and functionally characterized.

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Background: TIR1-like proteins are F-box auxin receptors. Auxin binding to the F-box receptor proteins promotes the formation of SCF(TIR1) ubiquitin ligase complex that targets the auxin repressors, Aux/IAAs, for degradation via the ubiquitin/26S proteasome pathway. The release of auxin response factors (ARFs) from their Aux/IAA partners allows ARFs to mediate auxin-responsive changes in downstream gene transcription.

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Background: Variations in floral display represent one of the core features associated with the transition from allogamy to autogamy in angiosperms. The promotion of autogamy under stress conditions suggests the potential involvement of a signaling pathway with a dual role in both flower development and stress response. The jasmonic acid (JA) pathway is a plausible candidate to play such a role because of its involvement in many plant responses to environmental and developmental cues.

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Biological activity of polyphenols is influenced by their uptake and is highly influenced by their interactions with the food matrix. This study evaluated the complex formation of blueberry polyphenols with fruit matrixes such as pectin and cellulose and their effect on the biological and antiproliferative properties of human colon cell lines HT-29 and CRL 1790. Free or complexed polyphenols were isolated by dialyzing aqueous or methanolic blueberry homogenates.

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Ethylene has long been considered the key regulator of ripening in climacteric fruit. Recent evidence showed that auxin also plays an important role during fruit ripening, but the nature of the interaction between the two hormones has remained unclear. To understand the differences in ethylene- and auxin-related behaviours that might reveal how the two hormones interact, we compared two plum (Prunus salicina L.

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Fruit growth is a coordinated, complex interaction of cell division, differentiation and expansion. Gibberellin (GA) involvement in the reproductive events is an important aspect of GA effects. Perennial fruit-trees such as plum (Prunus salicina L.

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