Management of aberrant crossing of superficial external pudendal artery during robotic video-endoscopic inguinofemoral lymphadenectomy: a video case study.

Inguinofemoral lymphadenectomy remains a critical component of staging and treatment for vulvar and penile squamous cell carcinoma. Traditionally performed via an open approach, this procedure is associated with significant morbidity, including lymphocyst formation, chronic lymphedema, and delayed recovery. A minimally invasive alternative, via laparoscopic or robotic platforms, is gaining traction as it is associated with a lower risk of surgical morbidity.

Femoral vein ligation for groin recurrence in a woman with vulval squamous cell carcinoma: A case report.

The management of groin recurrences in women with vulval cancer presents a significant therapeutic challenge. Groin recurrences, particularly those involving the femoral vessels, are associated with poor outcomes. Historically, surgery was often not offered in such cases, exposing patients to the risk of fatal complications from femoral vessel blow-out, leading to exsanguination.

Vulval Flap Reconstruction in Women With Benign, Preneoplastic and Malignant Vulval Conditions: A Prospective Study.

Objectives: To (i) evaluate the surgical morbidity, (ii) identify correlates of these and, (iii) explore whether flap reconstruction following vulvectomy improves patient symptoms and quality of life.

Design: Single arm prospective study.

Setting: Single tertiary vulval centre, UK.

Aberrant activation of the Hedgehog signalling pathway in squamous cell carcinoma of the vulva as a potential target for cancer therapy.

In a previous study, we showed that the Hedgehog (Hh) signalling pathway is aberrantly activated in vulval squamous cell carcinoma (VSCC). In this study, we further validated our findings on a prospective cohort of primary VSCC cases, where immunohistochemical staining confirmed that key Hh pathway components were overexpressed in VSCC compared to normal vulval epithelium. We also undertook a series of in vitro studies to determine the extent of Hh pathway activation in VSCC-derived cell lines, and examine the consequences of pathway inhibition on the growth of these cells.

The Major Constituent of Green Tea, Epigallocatechin-3-Gallate (EGCG), Inhibits the Growth of HPV18-Infected Keratinocytes by Stimulating Proteasomal Turnover of the E6 and E7 Oncoproteins.

Epigallocatechin-3-gallate (EGCG), the primary bioactive polyphenol in green tea, has been shown to inhibit the growth of human papilloma virus (HPV)-transformed keratinocytes. Here, we set out to examine the consequences of EGCG treatment on the growth of HPV18-immortalised foreskin keratinocytes (HFK-HPV18) and an authentic HPV18-positive vulvar intraepithelial neoplasia (VIN) clone, focusing on its ability to influence cell proliferation and differentiation and to impact on viral oncogene expression and virus replication. EGCG treatment was associated with degradation of the E6 and E7 oncoproteins and an upregulation of their associated tumour suppressor genes; consequently, keratinocyte proliferation was inhibited in both monolayer and organotypic raft culture.