Rapid and Esthetic Wound Healing in Surgery by Applying a Powdered Mixture of Mannose and Calcium Propionate.

Background: Wound healing is a current problem in surgery. Wounds most commonly occur from surgery, accidents, and burns. The purpose of this study was to reduce bleeding time and wound healing time as well as prevent or mitigate scarring by applying D-mannose with calcium propionate mixtures directly to the wound in powder form.

Accelerated wound healing and reduced scar formation induced by D-mannose: a possible role of mannose binding lectin.

Wounds are most commonly caused by accidents, surgery and burns, and can be internal or external. Naturally, the wound healing process can take a long time and lead to scarring. In this study we present a technique to shorten wound healing time and prevent or mitigate scarring using D-mannose that is applied directly on the wound.

Selective and fast methylation of free fatty acids directly in plasma for their individual analysis by gas chromatography- mass spectrometry.

An analytical procedure for the rapid and selective derivatization of free fatty acids into methyl esters directly in plasma without transmethylation of lipid-bound fatty acids was developed for their analysis by gas chromatography-mass spectrometry. The methyl esters of free fatty acids were obtained by reaction with methyl iodide in the solution of dipolar aprotic solvents and in the presence of solid bases. The mechanism of the methylation reaction with these reagents was investigated.

Simultaneous analysis of neutral monosaccharides, fatty acids and cholesterol as biomarkers from a drop of blood.

Aim: We have developed a method for simultaneous monitoring of more biomarkers from three different classes of compounds by simultaneous analysis of neutral monosaccharides, fatty acids (FAs) and cholesterol as their per-O-methylated derivatives from a drop of blood by GC-MS. This work is a development of our previous results about analysis of neutral monosaccharides from a drop of blood.

Methods & Results: The simultaneous per-O-methylation was obtained by methylation in one step with methyl iodide and NaOH in DMSO.

Predictive Models for Fast and Effective Profiling of Kinase Inhibitors.

In this study we developed two-dimensional pharmacophore-based random forest models for the effective profiling of kinase inhibitors. One hundred seven prediction models were developed to address distinct kinases spanning over all kinase groups. Rigorous external validation demonstrates excellent virtual screening and classification potential of the predictors and, more importantly, the capacity to prioritize novel chemical scaffolds in large chemical libraries.

Determination of Neutral Monosaccharides as Per-O-methylated Derivatives Directly from a Drop of Whole Blood by Gas Chromatography-Mass Spectrometry.

A new analytical procedure was developed for the simultaneous quantification of neutral monosaccharides from a drop of whole blood using gas chromatography-mass spectrometry analysis (GC-MS) of their per-O-methylated derivatives. The per-O-methylation reaction with methyl iodide and solid sodium hydroxide in methyl sulfoxide was used for the first time for analysis of blood monosaccharides. A blood drop volume of 0.

Solid phase microextraction assisted by droplets-based liquid-liquid microextraction for analysis of volatile aromatic hydrocarbons in water by gas chromatography.

A new technique for the analysis of volatile aromatic hydrocarbons by combining liquid-liquid microextraction with solid phase microextraction has been developed. The analytes were extracted from aqueous samples by an immobilized polydimethylsiloxane fiber assisted by the droplets of an appropriate organic solvent. Benzene, toluene, ethylbenzene, and o-xylene were used as target analytes.

Quantitative evaluation of perchlorethylene in groundwater before and after its oxidation by helical solid sorbent extraction and gas chromatography.

An integrated method of dynamic extraction of perchloroethylene (PCE) with polydimethylsiloxane (PDMS) helical solid sorbent followed by injection into a gas chromatograph was developed for the determination of the real concentration of PCE in groundwater before and after its degradation by oxidation with KMnO(4). The main parameters (agitation, temperature, salts, pH) that affect the extraction efficiency have been evaluated and optimized. The increase of the extracted amount of PCE due to the presence of the salts could be partially compensated by the opposite effect of the insoluble MnO(2), and of the presence of HCl and the global effect of the matrix would be less important for the reproducibility of the PCE extraction.

Per-O-methylation reaction for structural analysis of carbohydrates by mass spectrometry.

Per-O-methylation of carbohydrates is an important sample preparation step in structural analysis of complex carbohydrates, which has generated considerable interest as shown by thousands of citations in the last 10 years. This article provides a critical overview of the per-O-methylation methods applied for structural analysis of carbohydrates by mass spectrometry. The understanding of the O-methylation mechanism can help the researchers to apply the adequate O-methylation method and can generate new ideas in the effort of improving this reaction.

Per-O-methylation of neutral carbohydrates directly from aqueous samples for gas chromatography and mass spectrometry analysis.

Per-O-methylation of neutral carbohydrates in one step by adding dimethyl sulfoxide, powdered sodium hydroxide, and methyl iodide directly to aqueous sample is described. The influence of the water on the methylation reaction is investigated. Solid powdered sodium hydroxide is very hygroscopic and can scavenge the water from sample if an additional excess of sodium hydroxide is added.

Elimination of oxidative degradation during the per-O-methylation of carbohydrates.

The possible oxidative degradation mechanism occurring during the per-O-methylation of carbohydrates in dimethyl sulfoxide with methyl iodide in the presence of base is described. Evidence is presented that this process occurs only under anhydrous conditions when there is a long reaction time between the carbohydrate dissolved in dimethyl sulfoxide and methyl iodide, followed by reaction with the base. Under these specific conditions, the oxidative degradation of alditols, and cyclic carbohydrates, with and without a free hemiacetal group, is observed.

Helical sorbent microtrap for continuous sampling by a membrane and trap interface for on-line gas chromatographic monitoring of volatile organic compounds.

A helical sorbent microtrap consisting of a helical sorbent fixed inside a silicosteel capillary tube is presented. The main parameters that affect the safe sampling time of the helical sorbent microtrap in continuous sampling by a membrane and trap interface for on-line gas chromatographic monitoring of organic volatiles in gaseous samples are examined, taking into account the helical configuration of the sorbent, the presence of the membrane in system, and the properties of the analytes. Thermal desorption of analytes from the helical sorbent trap was also examined having regard to the influence of the turbulent flow generated by the helical sorbent in the heat transfer and the effect of thermal backward flow on the peak shape.

Helical sorbent for fast sorption and desorption in solid-phase microextraction-gas chromatographic analysis.

A new technique for solid-phase microextraction (SPME) of analytes using a helical solid sorbent followed by thermal desorption into a gas chromatographic injector is reported. The main factors that affect the mass transport of analytes in sorption and thermal desorption process using a poly(dimethylsiloxane) (PDMS) helical sorbent are described. The sorption and thermal desorption were achieved in a few seconds, being very close by the theoretical prediction.

Continuous permeation of analytes through a thin poly(dimethylsiloxane) membrane followed by sorbent trapping for their gas chromatographic monitoring.

Parameters that affect the permeation of analytes across a thin (0.025 mm) flat membrane of polysiloxane in a membrane and trap interface coupled to a gas chromatograph are examined taking into account a mechanism of mass transport for porous and nonporous materials and the permeation of carrier gas in the direction opposite to that of the analytes. The best permeation rate was reached with hydrogen as carrier gas at a flow-rate between 3 and 5 ml/min, an agitation of analytes outside the membrane above 0.

Continuous monitoring of thermooxidative degradation products of polystyrene by membrane extraction with sorbent interface and gas chromatography.

A novel method for the continuous monitoring of thermooxidative degradation products of polystyrene by membrane extraction with sorbent interface (MESI) and gas chromatography (GC) is developed. The results are compared with solid-phase microextraction-GC, which can extract gases, vapors, and aerosols. The volatile and semivolatile degradation products are identified by mass spectrometry.