Reproduction of on Onion and Potential Yield Suppression.

, is an emerging root-knot nematode species in the southern United States. To date, no studies have evaluated the host status of onions to . This study aimed to assess the reproduction and pathogenicity of on onion cultivars commonly grown in Georgia.

Copy number variations at the locus and their relationship with resistance to soybean cyst nematode ().

Soybean cyst nematode (SCN, ) is a devastating pest affecting soybean production worldwide. Host resistance is one of the primary practices used to manage SCN. The locus contributes to the strong and effective SCN resistance, with resistance levels predominantly governed by copy number variations (CNVs) and, to lesser extent, sequence variations.

Population Development of the Root-Lesion Nematode on Soybean Cultivars.

Two greenhouse experiments were conducted with the new species of root-lesion nematode, , discovered in North Dakota, USA in 2017 and named in 2021. Experiment 1 was conducted to ascertain resistance levels of soybean ( [L.] Merr.

Development of Real-Time and Conventional PCR Assays for Identifying a Newly Named Species of Root-Lesion Nematode () on Soybean.

A rapid and accurate PCR-based method was developed in this study for detecting and identifying a new species of root-lesion nematode ( ) recently discovered in a soybean field in North Dakota, USA. Species-specific primers, targeting the internal transcribed spacer region of ribosomal DNA, were designed to be used in both conventional and quantitative real-time PCR assays for identification of . The specificity of the primers was evaluated in silico analysis and laboratory PCR experiments.

Characterization of Virulence Phenotypes of Soybean Cyst Nematode () Populations in North Dakota.

Soybean cyst nematode (SCN; ) continues to be the greatest threat to soybean production in the United States. Because host resistance is the primary strategy used to control SCN, knowledge of SCN virulence phenotypes (HG types) is necessary for choosing sources of resistance for SCN management. To characterize SCN virulence phenotypes in North Dakota, a total of 419 soybean fields across 22 counties were sampled during 2015, 2016, and 2017.