Use of direct oral anticoagulants in patients with stroke transferred for thrombectomy.

Introduction And Objectives: Regional telestroke networks offer several advantages, including upstream selection of thrombectomy candidates. However, it is unclear whether the network performance and the functional outcome of patients with ischemic stroke treated with direct oral anticoagulants and transferred to thrombectomy differ from those of non-hypocoagulated patients. To compare the time metrics and the functional status of patients with ischemic stroke treated with direct oral anticoagulants and transferred to thrombectomy with non-hypocoagulated patients.

Hypertensive Diabetic Patients: Casual Pulse Pressure and Ambulatory Blood Pressure Monitoring (ABPM) as Superior Predictors of Future Cardiovascular Events.

Hypertensive (HTN) patients with type 2 diabetes mellitus (T2DM) are at an increased risk of adverse survival outcomes compared to non-diabetic individuals. This study aimed to retrospectively evaluate the prognostic significance of ambulatory blood pressure monitoring (ABPM) in a subgroup of diabetic patients. A total of 823 HTN patients, followed since 1994 at a hospital (follow-up: 11.

Peripheral Defocus in Orthokeratology Myopia Correction: Systematic Review and Meta-Analysis.

This study aimed to assess the effect of peripheral defocus with orthokeratology lenses (PDOK) on myopia control in children and adolescents through a systematic review and meta-analysis. A comprehensive search was conducted in the PubMed and Web of Science databases to identify randomized controlled trials (RCTs) and cohort studies on PDOK, using the keywords "peripheral refraction" and "orthokeratology". Studies were included if they reported spherical equivalent (M) peripheral refraction at 25° and/or 30° with accompanying statistical data along the horizontal meridian before and after orthokeratology treatment.

An Unexpected Case of Generalized Tetanus.

Tetanus is a disease of the nervous system caused by a toxin produced by , an anaerobe found in high concentrations in the soil. The occurrence of tetanus is related to contaminated traumatic wounds, and most patients have had some failure in their immunization. However, there are rare case reports of generalized tetanus in patients with proper vaccination schemes who failed to receive appropriate prophylaxis after high-risk exposure.

Trigeminal Nerve Schwannoma: A Rare Case of Multisymptomatic Cranial Nerve Involvement.

Schwannomas (SCs) are benign tumors composed of neoplastic Schwann cells and are relatively uncommon intracranially. Although these tumors are frequently associated with neurofibromatosis type 2 (NF2), they may also arise idiopathically, and their pathogenesis remains poorly understood. A 70-year-old Caucasian man presented with a two-month history of vertigo, gait imbalance, and decreased visual acuity in the left eye accompanied by photophobia, nausea, vomiting, and occasional headaches.

Arylsulfatases and neuraminidases modulate engagement of CCR5 by chemokines by removing key electrostatic interactions.

The chemokine receptor CCR5 is known to exist in cell surface subpopulations that differ in their capacity to engage ligands. One proposed explanation for this phenomenon is the presence of CCR5 species with different levels of post-translational modifications (PTMs). Tyrosine sulfation and O-glycan sialylation are PTMs that add negative charges to the extracellular domain of CCR5 and make strong contributions to chemokine binding but it is not known whether cellular mechanisms to control their levels exist.

Chitosan Nano/Microformulations for Antimicrobial Protection of Leather with a Potential Impact in Tanning Industry.

Tanned leather can be attacked by microorganisms. To ensure resistance to bacteria on leather surfaces, protection solutions need to be developed, addressing both environmental issues and economic viability. In this work, chitosan nano/microparticles (CNP) and chitosan/silver nano/microstructures (CSNP), containing silver nanoparticles around 17 nm size, were incorporated into leather, obtained from the industrial process.

Involving society in science: Reflections on meaningful and impactful stakeholder engagement in fundamental research.

Open Science calls for transparent science and involvement of various stakeholders. Here are examples of and advice for meaningful stakeholder engagement.

Corticosteroids in adult respiratory distress syndrome - an inconvenient truth?

Objectives: Recent studies have demonstrated mortality benefits from corticosteroid use in COVID-19 patients requiring respiratory support. However, clinical practice may warrant the use of corticosteroids outside the context of a clinical trial. Such data are rarely, if ever, reported.

Is it just an obstructive pyelonephritis?

Pyelocaliceal obstruction is a diagnostic challenge, and it is important to identify the obstruction cause. Some patients present extra-renal compressive masses that need further imagiologic investigation and a biopsy, to establish the diagnosis.

LifeTime and improving European healthcare through cell-based interceptive medicine.

Here we describe the LifeTime Initiative, which aims to track, understand and target human cells during the onset and progression of complex diseases, and to analyse their response to therapy at single-cell resolution. This mission will be implemented through the development, integration and application of single-cell multi-omics and imaging, artificial intelligence and patient-derived experimental disease models during the progression from health to disease. The analysis of large molecular and clinical datasets will identify molecular mechanisms, create predictive computational models of disease progression, and reveal new drug targets and therapies.

Thinking "ethical" when designing an international, cross-disciplinary biomedical research consortium.

This commentary outlines challenges with identifying and implementing ethical, legal and societal considerations when initiating large-scale scientific programs and suggests best practices to ensure responsible research.

Author Correction: In-cell identification and measurement of RNA-protein interactions.

An amendment to this paper has been published and can be accessed via a link at the top of the paper.

SPEN integrates transcriptional and epigenetic control of X-inactivation.

Xist represents a paradigm for the function of long non-coding RNA in epigenetic regulation, although how it mediates X-chromosome inactivation (XCI) remains largely unexplained. Several proteins that bind to Xist RNA have recently been identified, including the transcriptional repressor SPEN, the loss of which has been associated with deficient XCI at multiple loci. Here we show in mice that SPEN is a key orchestrator of XCI in vivo and we elucidate its mechanism of action.

In-cell identification and measurement of RNA-protein interactions.

Regulatory RNAs exert their cellular functions through RNA-binding proteins (RBPs). Identifying RNA-protein interactions is therefore key for a molecular understanding of regulatory RNAs. To date, RNA-bound proteins have been identified primarily through RNA purification followed by mass spectrometry.

X chromosome inactivation: new players in the initiation of gene silencing.

X chromosome inactivation (XCI) is a dosage compensation process that was adopted by female mammals to balance gene dosage between XX females and XY males. XCI starts with the upregulation of the non-coding RNA Xist, after which most X-linked genes are silenced and acquire a repressive chromatin state. Even though the chromatin marks of the inactive X have been fairly well described, the mechanisms responsible for the initiation of XCI remain largely unknown.

Alterations of Nuclear Architecture and Epigenetic Signatures during African Swine Fever Virus Infection.

Viral interactions with host nucleus have been thoroughly studied, clarifying molecular mechanisms and providing new antiviral targets. Considering that African swine fever virus (ASFV) intranuclear phase of infection is poorly understood, viral interplay with subnuclear domains and chromatin architecture were addressed. Nuclear speckles, Cajal bodies, and promyelocytic leukaemia nuclear bodies (PML-NBs) were evaluated by immunofluorescence microscopy and Western blot.

Prdm3 and Prdm16 are H3K9me1 methyltransferases required for mammalian heterochromatin integrity.

Heterochromatin serves important functions, protecting genome integrity and stabilizing gene expression programs. Although the Suv39h methyltransferases (KMTs) are known to ensure pericentric H3K9me3 methylation, the mechanisms that initiate and maintain mammalian heterochromatin organization remain elusive. We developed a biochemical assay and used in vivo analyses in mouse embryonic fibroblasts to identify Prdm3 and Prdm16 as redundant H3K9me1-specific KMTs that direct cytoplasmic H3K9me1 methylation.

Conjugation of human topoisomerase 2 alpha with small ubiquitin-like modifiers 2/3 in response to topoisomerase inhibitors: cell cycle stage and chromosome domain specificity.

Type 2 topoisomerases, in particular the alpha isoform in human cells, play a key role in cohesion and sister chromatid separation during mitosis. These enzymes are thus vital for cycling cells and are obvious targets in cancer chemotherapy. Evidence obtained in yeast and Xenopus model systems indicates that conjugation of topoisomerase 2 with small ubiquitin-like modifier (SUMO) proteins is required for its mitotic functions.

Mutagenic effects of poly (ADP-ribose) polymerase-1 deficiency in transgenic mice.

Poly (ADP-ribose) polymerase-1 (Parp1) plays a central role in the maintenance of genomic integrity and has been unequivocally associated to DNA base excision repair (BER) but its involvement in double-strand break (DSB) repair pathways remains unclear. In this work, using transgenic Parp1-deficient mice harbouring the lacZ reporter gene, we provide in vivo evidence that Parp1 contributes to the prevention of deletions/insertions in testis following an alkylation insult. In response to N-Methyl-N-Nitrosurea (MNU) treatment no significant difference in the mutant frequency (MF) in the liver and testis could be attributed to Parp1 status, given that both Parp1(+/+) and Parp1(-/-) mice showed a similar significant increase in the overall MF.