Dual-Toxin-Producing Strain Isolated from a Foodborne Botulism Case in Korea: Genomic and Functional Insights.

produces one of the most potent biological toxins and causes botulism, a rare but potentially fatal neuroparalytic disease. In 2014, a foodborne botulism case was reported in Korea, and a strain (CB-2014001) was isolated. Initial characterization identified it as a BoNT/B-producing strain based on mouse bioassay and conventional PCR.

Serological correlates of protection and long-term efficacy of a novel recombinant protective antigen-based anthrax vaccine in a rabbit model.

The novel anthrax vaccine candidate GC1109, composed of recombinant protective antigen, has shown robust antibody responses and safety in preclinical and clinical studies. However, the assessment of GC1109 vaccine efficacy was limited in a rodent model and could not be applied in clinical studies due to ethical issues. In this study, we aim to provide predictive insights for human applications of GC1109 by examining the correlation between anthrax toxin-neutralizing antibodies (TNAs) and protection against Bacillus anthracis infection in rabbit.

Antimicrobial Resistance Profiles of Isolated from Food Animal Carcasses During 2010-2023 in South Korea.

Antimicrobial-resistant bacterial contamination of meat poses a significant global public health risk. We aimed to determine antimicrobial resistance profiles and trends of recovered from carcasses of healthy food-producing animals in South Korea during 2010-2023. In total, 4748 isolates obtained from cattle ( = 1582), pigs ( = 1572), and chickens ( = 1594) were assessed for susceptibility to 12 antimicrobials.

Initial experience with the enhanced recovery after surgery (ERAS) protocols in gynecologic surgery at an urban academic tertiary medical center.

Background: The enhanced recovery after surgery (ERAS) protocols have been consistently associated with improved patient experience and surgical outcomes. Despite the release of ERAS Society guidelines specific to gynecologic oncology, the adoption of ERAS in gynecology on global level has been disappointingly low and some centers have shown minimal improvement in clinical outcomes after adopting ERAS. The aim of this study is to describe the development and early experience of ERAS protocols in gynecologic surgery at an urban academic tertiary medical center.

Antibiotic Resistance Profiles and Molecular Characteristics of -Carrying Serovar Albany Isolated from Chickens During 2013-2020 in South Korea.

The production of β-lactamase by nontyphoidal has become a public health issue throughout the world. In this study, we aimed to investigate the antimicrobial resistance profiles and molecular characteristics of β-lactamase-producing serovar Albany isolates. A total of 434 Albany were obtained from feces and carcasses of healthy and diseased food-producing animals [cattle ( = 2), pigs ( = 3), chickens ( = 391), and ducks ( = 38)] during 2013-2020.

Antimicrobial Resistance Profiles and Molecular Characteristics of Extended-Spectrum β-Lactamase-Producing Isolated from Healthy Cattle and Pigs in Korea.

Antimicrobial-resistant bacteria isolated from food animals pose a major health threat to the public on this planet. This study aimed to determine the susceptibility profiles of isolated from cattle and pig fecal samples and investigate the molecular characteristics of extended-spectrum β-lactamase (ESBL)-producing using gene identification, conjugation, and Southern blot approach. Overall 293 were recovered from cattle (120 isolates) and pigs (173 isolates) in 7 provinces of Korea during 2017-2018.

Antibiotic Resistance Profiles and Molecular Characteristics of -Carrying Serovar Enteritidis Isolates from Healthy and Diseased Chickens in Korea.

Extended-spectrum β-lactamase (ESBL)-producing serovar Enteritidis has emerged as a public health concern. The main objectives of this study were therefore to determine the antimicrobial susceptibility profiles of Enteritidis and to investigate the molecular characteristics of identified ESBL-producing isolates. In the study, 237 Enteritidis isolates (232 isolates from chickens, 4 from cattle, and 1 from a pig) were recovered from carcasses and fecal samples of healthy and diseased food animals between 2010 and 2017.

Bacterial Prevalence in Skin, Urine, Diarrheal Stool, and Respiratory Samples from Dogs.

The emergence of bacterial infections in companion animals is a growing concern as humans can also be infected through the transmission of pathogenic bacteria. Because there have been few studies conducted on companion animals, the extent and significance of prevalence in veterinary practices remain unknown. This is the first nationwide surveillance report aimed at elucidating the prevalence pattern and associated infections of isolated bacteria from dogs in Korea.

Antimicrobial Resistance in Isolates from Healthy Food Animals in South Korea, 2010-2020.

Antimicrobial-resistant bacteria in food animals pose a major public health threat worldwide. In this study, we aimed to assess the antimicrobial resistance profiles and resistance trends of commensal Escherichia coli isolated from the feces of healthy cattle, pigs, and chickens in South Korea during 2010 and 2020. A total of 7237 E.

Prevalence of Bacterial Species in Skin, Urine, Diarrheal Stool, and Respiratory Samples in Cats.

Bacterial infections are a significant cause of illness and death in different animals. However, these bacterial infections could be a source of human disease or illness if these pathogenic bacteria are present in companion animals. This study aimed to investigate the prevalence of pathogenic bacteria associated with different site infections in cats in the Republic of Korea.

Development and Evaluation of AccuPower COVID-19 Multiplex Real-Time RT-PCR Kit and AccuPower SARS-CoV-2 Multiplex Real-Time RT-PCR Kit for SARS-CoV-2 Detection in Sputum, NPS/OPS, Saliva and Pooled Samples.

Rapid and accurate detection of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is essential for the successful control of the current global COVID-19 pandemic. The real-time reverse transcription polymerase chain reaction (Real-time RT-PCR) is the most widely used detection technique. This research describes the development of two novel multiplex real-time RT-PCR kits, AccuPower® COVID-19 Multiplex Real-Time RT-PCR Kit (NCVM) specifically designed for use with the ExiStation™48 system (comprised of ExiPrep™48 Dx and Exicycler™96 by BIONEER, Korea) for sample RNA extraction and PCR detection, and AccuPower® SARS-CoV-2 Multiplex Real-Time RT-PCR Kit (SCVM) designed to be compatible with manufacturers' on-market PCR instruments.

Imipenem Resistance Mediated by Gene in .

Treatment of infectious diseases caused by carbapenem-resistant is becoming a greater challenge. This study aimed to identify the imipenem resistance mechanism in isolated from a dog. Minimum Inhibitory Concentration (MIC) was determined by the broth microdilution method according to the Clinical and Laboratory Standards Institute recommendations.

Antimicrobial Resistance Profiles and Macrolide Resistance Mechanisms of Isolated from Pigs and Chickens.

We identified 1218   isolates from fecal and carcass samples of pigs ( = 643) and chickens ( = 575) between 2010 and 2018. About 99% of the isolates were resistant to at least one antimicrobial agent. The isolates exhibited high resistance rates (>75%) to ciprofloxacin, nalidixic acid, and tetracycline.

Nationwide Surveillance on Antimicrobial Resistance Profiles of and Isolated from Healthy Food Animals in South Korea, 2010 to 2019.

Intestinal commensal bacteria are considered good indicators for monitoring antimicrobial resistance. We investigated the antimicrobial resistance profiles and resistance trends of and isolated from food animals in Korea between 2010 and 2019. and isolated from chickens and pigs, respectively, presented a relatively high resistance rate to most of the tested antimicrobials.

Nationwide Surveillance on Antimicrobial Resistance Profiles of Isolated from Major Food Animal Carcasses in South Korea During 2010-2018.

Contamination of meat with antimicrobial-resistant bacteria represents a major public health threat worldwide. In this study, we determined the antimicrobial resistance profiles and resistance trends of isolated from major food animal carcasses (408 cattle, 1196 pig, and 1312 chicken carcass isolates) in Korea from 2010 to 2018. Approximately 75%, 92%, and 77% of cattle, pig, and chicken carcass isolates, respectively, were resistant to at least one antimicrobial agent.

Mobile Colistin Resistance Gene - Detected on an IncI2 Plasmid in Typhimurium Sequence Type 19 from a Healthy Pig in South Korea.

Colistin is considered the last resort for the treatment of multi-drug resistant Gram-negative bacterial infections. We studied colistin resistance and the gene carriage in isolates recovered from food animals in South Korea between 2010 and 2018. Colistin resistance was found in 277 isolates, predominantly in Enteritidis (57.

Short communication: First detection of Panton-Valentine leukocidin-positive methicillin-resistant Staphylococcus aureus ST30 in raw milk taken from dairy cows with mastitis in South Korea.

We identified 199 Staphylococcus aureus isolates from quarter milk samples of 1,289 dairy cattle between 2014 and 2018. About 66% of the isolates were resistant to at least 1 antimicrobial agent; the highest rate of resistance was to penicillin, followed by resistance to ampicillin, erythromycin, and sulfadimethoxine. We obtained 30 methicillin-resistant S.

Emergence of -Mediated Linezolid Resistance in Isolated from Pig Carcasses.

Altogether, 2547 isolated from cattle ( = 382), pig ( = 1077), and chicken carcasses ( = 1088) during 2010-2017 were investigated for linezolid resistance and were further characterized using molecular methods. We identified linezolid resistance in only 2.3% of pig carcass isolates.

First Report of an Strain Carrying the Colistin Resistance Determinant from a Dog in South Korea.

We studied the presence of the mobile colistin resistance gene - in isolates recovered from fecal and urine samples of companion animals, that were collected from South Korea in 2018 and 2019. The - gene was detected in one colistin-resistant . isolated from a diarrheic dog.

Resistance profiling and molecular characterization of Staphylococcus aureus isolated from goats in Korea.

Staphylococcus aureus is among the most common zoonotic pathogens that cause foodborne illnesses worldwide. The main objectives of the current study were therefore to determine the antimicrobial susceptibility profiles of S. aureus isolated from goats in Korea and to investigate the molecular characteristics of identified methicillin-resistant S.

Emergence of in Diseased Pigs in South Korea.

We examined the prevalence and molecular characteristics of carrying colistin-resistant among cattle, pig, and chicken isolates in South Korea. Among a total of 185 colistin-resistant isolates determined in this study (47 from cattle, 90 from pigs, and 48 from chicken), PCR amplification detected - genes in 17 isolates predominantly from diseased pigs. The - genes were characterized as in 15 isolates and - in 2 isolates.

Nationwide surveillance on serotype distribution and antimicrobial resistance profiles of non-typhoidal Salmonella serovars isolated from food-producing animals in South Korea.

Food-producing animals are considered a leading source of human Salmonella infections in Korea. However, there is a lack of comprehensive and up-to-date data regarding the diversity and resistance profiles of Salmonella serotypes in these animals. Therefore, this study aimed to determine the distribution and antimicrobial resistance profiles of Salmonella serotypes isolated from cattle, pigs, and chickens in Korea between 2010 and 2018.

Resistance Profiling and Molecular Characterization of Extended-Spectrum/Plasmid-Mediated AmpC β-Lactamase-Producing Isolated from Healthy Broiler Chickens in South Korea.

We aimed to identify and characterize extended-spectrum β-lactamase (ESBL)-and/or plasmid-mediated AmpC β-lactamase (pAmpC)-producing isolated from healthy broiler chickens slaughtered for human consumption in Korea. A total of 332 isolates were identified from 339 cloacal swabs in 2019. More than 90% of the isolates were resistant to multiple antimicrobials.