Epstein-Barr virus-associated intrahepatic cholangiocarcinoma: a diverse morphological spectrum that correlates with patient prognosis.

Epstein-Barr virus (EBV)-associated intrahepatic cholangiocarcinoma (EBVaICC) is a rare condition with limited comprehensive descriptions of its clinicopathological features. This study investigated the wide morphological spectrum of EBVaICC to improve diagnostic accuracy and explored the associations between morphological characteristics and prognosis. Nineteen cases of EBVaICC were retrospectively analysed, and 102 cases reported in the literature were reviewed.

Targeting PBK with small-molecule 1--acetyl-4,6-britannilactone for the treatment of neuroinflammation.

Neuroinflammation is a complex immunological phenomenon characterized by a dysregulated inflammatory response in the central nervous system (CNS) that can be triggered by various pathological injuries, such as toxins, which are involved in Parkinson's and Alzheimer's diseases (PD and AD), therefore, suppressing neuroinflammation serves as an effective treatment for CNS diseases. Herein, we found that natural soluble epoxide hydrolase (sEH) inhibitor 1--acetyl-4,6-britannilactone (AB) regulated mitogen-activated protein kinase (MAPK) and AMP-activated protein kinase (AMPK) pathways to suppress the microglial activation by regulating inflammation and autophagy in vitro and in vivo, contributing to the improvement of lipopolysaccharide (LPS)-mediated neuroinflammation. Protein microarray analysis indicated that AB could selectively target PDZ binding kinase (PBK) through covalently binding to C70, exhibiting a dissociation constant (Kd) of 0.

Andrographolide and its sulfated metabolite alleviated DSS-induced inflammatory bowel disease through regulating inflammation and immune via MAPK/NLRP3 pathways and the balance of Th17/Treg cells.

Inflammatory Bowel Disease (IBD), is a chronic illness characterized by severe abdominal pain, diarrhea, and weight loss, seriously diminishing patients' quality of life. Andrographolide (AND), a natural diterpenoid from Andrographis paniculata, and its sulfated metabolite, andrographolide sodium bisulfite (ASB), have showed potential anti-inflammatory effects. However, their mechanism in IBD remains elusive.

Differences in Fatty Acid Metabolism between MCDD and HFD Induced Metabolic Dysfunction-associated Fatty Liver Disease Model Mice.

Background: The global incidence of metabolic dysfunction-associated fatty liver disease (MAFLD) is increasing annually, which has become a major public-health concern. MAFLD is typically associated with obesity, hyperlipemia, or metabolic syndrome. Dietary induction is one of the most common methods for preparing animal models of MAFLD.

Discovery of soluble epoxide hydrolase inhibitors from Inula britannica: Inhibition kinetics, molecular dynamics simulation, biochemical, and in vitro cell-based studies.

Soluble epoxide hydrolase (sEH) is an enzyme involved in transforming epoxyeicosatrienoic acids (EETs) to dihydroxyeicosatrienoic acids (DHETs). EETs play a pivotal role in maintaining anti-inflammatory response and pain regulation, making sEH as an important target for therapeutic interventions. Based on our present study of discovery of sEH inhibitors, herein, six new (1-6) and fifteen known compounds (7-21) with inhibitory effects toward sEH were isolated from Inula britannica.

In vivo Pharmacokinetic/pharmacodynamic relationship of florfenicol in combination with doxycycline against Riemerella anatipestifer in ducks and the effect upon resistance development.

Antimicrobial chemotherapy is necessary to control Riemerella anatipestifer (RA), among which florfenicol (FF) is regarded as one of the preferred options. Based on the consideration of drug combination to improve efficacy, the pharmacokinetics and pharmacodynamics of FF combined with doxycycline (DOX) against RA were studied. FF was administered at doses of 20 or 40 mg/kg in combination with DOX (1, 2.

Subcycle spectral structures of light-induced states in attosecond transient absorption of helium dressed by an orthogonally polarized laser field.

We present the subcycle spectral structures from attosecond transient absorption spectra of helium by accurately solving the full three-dimensional time-dependent Schrödinger equation in extreme ultraviolet attosecond pulse and an orthogonally polarized infrared (IR) laser field. We discover that the subcycle spectral features associated with the dressed 1 ( ≥ 4) states and light-induced states, referred to as , , and , can be strongly modified or even enhanced when the strength ratio of the orthogonal laser field in two polarized laser directions changes. To understand the spectral evolution of the subcycle structures, we perform calculations of the time-dependent population and time-frequency analysis.

In vivo pharmacokinetic and pharmacodynamic study and cutoff of florfenicol against Riemerella anatipestifer in ducks.

Riemerella anatipestifer causes serious infections, characterized by septicemia and serositis, in ducks and geese. R. anatipestifer is mainly controlled through antimicrobial chemotherapy.

Sesquiterpenoids from Inula britannica and their potential mechanism for immunomodulation.

The immune system serves as a role of diseases, such as Parkinson's disease, and acute lung injury. An immunoregulatory activity-directed separation depended on phorbol 12-myristate 13-acetate (PMA) plus ionomycin (Ion)-mediated Jurkat leukemic T cells was used for studying chemical constituents from Inula britannica L. in depth.

Aucklandiae radix targeted PKM2 to alleviate ulcerative colitis: Insights from the photocrosslinking target fishing technique.

Background: Inflammatory bowel disease (IBD) is a chronic and relapsing disease marked by chronic tissue inflammation that alters the integrity and function of the gut, seriously impacting patient health and quality of life. Aucklandiae Radix (AR), known as Mu Xiang in Chinese, is a traditional Chinese medicine documented in Chinese Pharmacopoeia with effects of strengthening the intestine and stopping diarrhea. However, the potential of AR in treating intestinal inflammation and its underlying mechanism have yet to be further elucidated.

Effects of Glycosylation Combined with Phosphate Treatment on the Allergenicity and Structure of Tropomyosin in .

Tropomyosin (TM) is the main allergen in shrimp (). In this study, the effects of allergenicity and structure of TM by glycosylation (GOS-TM), phosphate treatment (SP-TM), and glycosylation combined with phosphate treatment (GOS-SP-TM) were investigated. Compared to GOS-TM and SP-TM, the IgG/IgE binding capacity of GOS-SP-TM was significantly decreased with 63.

Effects of Reducing Sugars on the Structural and Flavor Properties of the Maillard Reaction Products of Seed Meal.

seed meal contains a variety of bioactive compounds, but the use of seed meal in the food industry is rare. This study aimed to evaluate the effect of reducing sugars on the structural and flavor properties of the Maillard reaction products (MRPs) of the seed meal hydrolysate (LSH). The results showed that the flavors and tastes of the MRPs were affected by reducing sugars.

Based on metabolomics, the optimum wind speed process parameters of flue-cured tobacco in heat pump bulk curing barn were explored.

To explore the influence of wind speed on the quality of tobacco in this study, we employed a heat pump-powered intensive curing barn and a three-stage curing process. By evaluating the influence of fan parameters on the quality of tobacco leaves at different curing stages, the optimal wind speed was determined. After adopting the optimized wind speed process, the degradation of macromolecular substances was faster, the accumulation of aroma substances was delayed to 55 °C, and the accumulation was more complete.

Membrane location of cardiolipin antigen in : further study on the origin of nontreponemal antibodies.

The present study examined the membrane location of cardiolipin antigen in treponemes. The authors used different methods to disrupt the outer membrane of treponemes, detected the location of the cardiolipin antigen and analyzed the immune response in rabbits immunized with various antigens. All organisms were labeled with nontreponemal antibodies on immunoelectron and fluorescence microscopy, except the citrate buffer-treated group, which is a method leading to relatively complete removal.

Therapeutic Drug Monitoring of Ceftazidime-Avibactam Concentrations in Carbapenem-Resistant Infected Patients With Different Kidney Statuses.

Carbapenem-resistant (CRKP) is the most common carbapenem-resistant with high mortality. Ceftazidime-avibactam (CAZ-AVI) has exhibited excellent activity against CRKP. However, the efficacy of CAZ-AVI in KPC-producing CRKP-infected patients with different kidney statuses varies, such as renal insufficiency, normal renal function, and augmented renal clearance (ARC).

Identification of specific protein biomarkers in syphilis patient serum using mass spectrometry.

To screen novel biomarkers in serum of syphilis patients using a mass spectrometry-based method. Sera were collected from 18 syphilis patients and divided into three groups. Every six serum samples (before and after treatment) in each group were pooled and detected by mass spectrometry.

Effects of Disease-Related Knowledge on Illness Perception and Psychological Status of Patients With COVID-19 in Hunan, China.

Objective: The aim of this study was to assess the current status of disease-related knowledge and to analyze the relationship among the general condition, illness perception, and psychological status of patients with coronavirus disease 2019 (COVID-19).

Methods: A hospital-based cross-sectional study was conducted on 118 patients using convenience sampling. The general questionnaire, disease-related knowledge questionnaire of COVID-19, Illness Perception Questionnaire (IPQ), and Profile of Mood States (POMS) were used to measure the current status of participants.

Characterisation of the novel clinical isolate X-4 containing a new sequence-type.

Objectives: A novel sequence-type was identified in one clinical isolate (X-4) from a patient diagnosed with primary syphilis in Xiamen, China. To precisely define and characterise a new clinical isolate, we performed further genome-scale molecular analysis.

Methods: The pooled segment genome sequencing method followed by Illumina sequencing was performed.

Immunization with nontreponemal antigen alters the course of experimental syphilis in the rabbit model.

The role of nontreponemal antibodies in the Treponema pallidum infection course is unclear. We investigated the effect of immunization with nontreponemal antigen on T. pallidum-challenged rabbits.

Tp0136 targets fibronectin (RGD)/Integrin β1 interactions promoting human microvascular endothelial cell migration.

Lesion healing without treatment is a unique clinical characteristic of the early stages of syphilis infection. Angiogenesis, which involves endothelial cell migration, is an important process in wound healing. Tp0136, an outer membrane protein of T.

A chemiluminescence immunoassay for the detection of NT-proBNP.

Background: The aim of the study was to assess the analytical performance of the HISCL NT-proBNP assay, a newly developed chemiluminescence immunoassay, for the detection of NT-proBNP.

Methods: The within-run and total imprecision of the NT-proBNP assay were determined with HISCL cardiac marker controls. The linear ranges of the NT-proBNP assays were evaluated based on the CLSI EP6-A document using selected serum samples.