Study of gastro-intestinal strongylosis in a sheep flock on permanent pasture 2. Sheep parasitism in 1978-1979.

The epidemiological study carried out in 1977 in the Limousin region of France was continued for the next two years. Eighty-five lambs were slaughtered at the rate of five animals every weeks: sixty were slaughtered from May 1978 to March 1979 and twenty-five from May to September 1979. Total parasite counts and coprological examinations were carried out.

New data on the in-situ position of the inactive X chromosome in the interphase nucleus of human fibroblasts.

The in situ spatial distribution of nucleolus-organizing-region (NOR) bearing chromosomes in relation to the inactive X chromosome was studied during interphase in human fibroblasts. The respective positions of these chromosomes were examined in 30 growing and 32 resting fibroblasts from reconstructed nuclei, using nucleoli and the Barr body as ultrastructural markers. Experimental values for the distance between the nucleoli and the Barr body were estimated by their coefficient of closeness and compared to the uniform distribution.

[LHRH stimulates the secretion of an 87 K protein by cultured gonadotrophic cells].

The biosynthesis of proteins secreted by cultured pituitary cells was studied using (35S)-methionine. The intracellular and released proteins were analyzed by polyacrylamide gel electrophoresis followed by autoradiography. The LHRH-stimulated release is accompanied by the synthesis and release of a 87,000 dalton molecule (87 K protein).

In situ ultrastructural localization of sugar-binding sites in lizard granulosa cell nuclei.

Mannose-binding sites were detected at the ultrastructural level in nuclei of lizard granulosa cells in situ by means of mannosylated ferritin. When ultrathin sections of material embedded in glycol methacrylate were incubated with mannosylated ferritin, a strong labelling was observed over nucleoli, chromatin and the external leaflet of the nuclear envelope, but no labelling was detected in the perinuclear space except for nuclear pores. This labelling was clearly inhibited when sections were incubated in a solution containing both mannosylated ferritin and a sugar-related neoglycoprotein (mannosylated serum albumin).

Use of a monoclonal antibody to quantify (Na+,K+)-ATPase activity and sites in normal and regenerating rat liver.

Quantitative measurements of (Na+,K+)-ATPase activity and numbers of (Na+,K+)-ATPase sites in membranes from quiescent and regenerating rat liver have been made using an anticatalytic monoclonal antibody (9-A5) that binds to alpha subunits of the sodium pump (Schenk, D. B., and Leffert, H.

Yeast regulatory gene PPR1. I. Nucleotide sequence, restriction map and codon usage.

The PPR1 gene of Saccharomyces cerevisiae controls the transcription of two unlinked structural genes URA1 and URA3. The primary structure of this eukaryotic regulatory gene and its flanking regions has been established by the dideoxynucleotide chain termination method. Our data show an open reading frame of 2712 nucleotides, corresponding to 904 amino acid residues.

Possible role for a human adenovirus in the pathogenesis of celiac disease.

Celiac disease in humans is activated by the dietary ingestion of wheat, rye, triticale, barley, and possibly oats. Gliadins in wheat and similar proteins in the other grains are known to activate disease in susceptible individuals. There is a striking association between celiac disease and HLA-B8, -DR3 and/or -DR7, and -DC3.

In situ distribution in different cell types of nuclear glycoconjugates detected by two lectins.

Nuclear glycoconjugates were detected in situ by two lectins--Concanavalin A and Wheat germ agglutinin--on tissue sections embedded in the hydrophilic resin glycol methacrylate. These lectins were conjugated with fluorescein isothiocyanate for fluorescence microscopy, and labelled with ferritin for electron microscopy. The ovarian follicle of the lizard Lacerta vivipara was chosen for this study, because it enables four types of nuclei, with different ultrastructures and physiology to be observed on the same section.

Three-dimensional approaches to the residual structure of histone-depleted HeLa cell nuclei.

Histone-depleted nuclei were prepared by high-salt extraction of interphase HeLa cell nuclei. A large amount of the nuclear DNA remained associated with a rapidly sedimenting residual nuclear structure including cytoplasmic (intermediate filament) and nuclear (matrix and lamina) proteins. Electron microscopy allowed detection in the insoluble structure of a residual nuclear envelope, nucleolar residues, and an intranuclear network whose correspondence with components of in situ fixed nuclei is discussed.

A new method for culture of larvae used in diagnosis of ruminant gastrointestinal strongylosis: comparison with fecal cultures.

A study was carried out in order to compare two techniques for culture of strongyle larvae: the usual fecal culture and a new one where eggs were extracted from feces and larvae were grown in a more defined medium (agar plate fortified with Earle's medium and yeast extract). The efficiency of "on-agar" cultures was better than that of the fecal cultures with a difference of 26% for Trichostrongylus colubriformis and 32% for Ostertagia circumcincta. The variability observed between the number of larvae collected from each culture was low (8% on average) in comparison with that of fecal cultures (15% on average) thus demonstrating a better reproducibility.

[Compression of the brachial plexus by a pseudarthrosis of the 1st rib].

The authors have described the case of a thoracic outlet syndrome with costoclavicular compression of the brachial plexus, secondary to non-union of a first rib fracture. Treatment consisted in transaxillary removal of the first rib, as described by Roos. Intrafascicular neurolysis of the posterior and medial trunks was performed at the same time, and was followed by quick clinical and electromyographical recovery.

Anchorage of the nucleolus in the pore complex-lamina by a DNA-bearing structure masked in situ in rat liver nuclei.

We have developed a method by which nuclear shells containing nucleoli can be isolated from membrane-depleted rat liver nuclei. This method involves the removal of the internal chromatin. This chromatin is expelled from the nuclear shell using combinations of low and high ionic strength buffers.

[Roos' axillary approach to the brachial plexus].

An anatomic study of five cadavers, that were dissected on a level with the two shoulders, was made to explore the possibilities of the Roos ' axillary surgical approach. This surgical approach permits almost complete resection of the first rib and dissection of plexus brachialis's inferior roots. (T1, C8 and C7).

Dog and human acid beta-D-galactosidases are structurally similar.

The purification of dog liver acid beta-galactosidase is described. The dog enzyme migrated as a single major band on polyacrylamide-gel electrophoresis in the presence of sodium dodecyl sulphate, with a molecular weight of 60000. Antiserum raised against purified human liver acid beta-galactosidase cross-reacted with beta-galactosidase from dog liver, but not with those from cat liver or Escherichia coli.

Comparison of liver alcohol dehydrogenases in Fischer-344 and Sprague-Dawley rats.

Livers of Sprague-Dawley rats contain 30-100% more alcohol dehydrogenase activity than livers of Fischer-344 rats. When weight-matched rats from both strains are injected with the same dose of ethanol (1.2 g/kg), Sprague-Dawley rats achieve lower blood alcohol levels than Fischer-344 rats at all the time-points tested.

Complete sequence of a eukaryotic regulatory gene.

Dihydroorotase, the third enzymatic activity of the pyrimidine pathway, is encoded in Saccharomyces cerevisiae by a single gene URA4, which is induced at the transcriptional level by accumulation of ureidosuccinic acid. A regulatory gene PPR2 (pyrimidine pathway regulatory 2) acting specifically on this step, has been characterized, cloned and sequenced. The main open reading frame is 384 nucleotides long and potentially codes for a basic protein, favoring a molecular mechanism involving direct binding of a regulatory protein to DNA.

Effect of infarct location on the in-hospital prognosis of patients with first transmural myocardial infarction.

We studied the in-hospital prognosis of 1105 patients who had their first transmural myocardial infarction; 611 patients (55.3%) had anterior myocardial infarction (AMI) and 494 (44.7%) had inferior myocardial infarction (IMI).

Cloning and restriction mapping of the yeast URA2 gene coding for the carbamyl phosphate synthetase aspartate-transcarbamylase complex.

Two yeast DNA pools inserted in a hybrid Escherichia coli-yeast vector pFL1 were used to transform E. coli and yeast aspartate-transcarbamylase-less strains to prototrophy. From the first pool--a BamHI yeast DNA digest--a 6.