Purification of active staphylococcal alpha-toxin produced in Bacillus subtilis.

The Gram-positive bacterium Staphylococcus aureus, a multidrug-resistant pathogen, poses a significant threat to human and animal health. Its virulence is largely attributed to the production of alpha-toxin (Hla), a potent cytotoxic protein that forms pores in cell membranes, leading to lysis. This is the first study to report the successful expression and purification of wild-type Hla (HlaWT) using Bacillus subtilis-a safe, genetically stable, and endotoxin-free host system.

Oral administration of Bacillus subtilis spores expressing Staphylococcus aureus IsdB induces mucosal immune responses in mice.

Purpose: Bacillus subtilis spores, known for their durability and well-developed genetic manipulation tools, show promise for oral vaccine delivery. Staphylococcus aureus is a major global health concern due to multidrug resistance and lack of a vaccine. This study explored the potential of B.

Sortase-mediated surface display of a chimeric immunogen on Bacillus subtilis: a chicken necrotic enteritis vaccine candidate.

The application of sortase-mediated surface display technology on Bacillus subtilis represents a novel approach in protein engineering. We developed and evaluated a recombinant chimeric protein (R-anz) integrating truncated forms of three Clostridium perfringens toxins (CPA, NetB, ZMP) displayed on B. subtilis.

Alteration of perineuronal nets and parvalbumin interneurons in prefrontal cortex and hippocampus, and correlation with blood corticosterone in activity-based anorexia model mice.

Anorexia nervosa (AN) is an eating disorder characterized by restricted energy intake, severely underweight status, and frequent hyperactivity. Previous research has shown structural and functional alterations in the medial prefrontal cortex (mPFC) and hippocampus of AN patients. To investigate the pathological mechanism of AN, we analyzed the expression and distribution of parvalbumin (PV) interneurons and perineuronal nets (PNNs), which are implicated in the pathology of neuropsychiatric disorders, in the mPFC and hippocampus dorsal (HPCd) and ventral (HPCv) using an activity-based anorexia (ABA) mouse model.

Comparative chloroplast genomic analysis of Pithecellobium dulce (Roxb.) Benth 1844 and related species within Caesalpinioideae.

The genus Pithecellobium Mart. (Caesalpinioideae, Fabaceae) plays vital ecological roles, including nitrogen fixation and habitat stabilization, and holds significant medicinal and economic values. However, its genetic diversity and evolutionary relationships remain poorly understood.

Imaging oligomers of alpha-toxin (Hla) variants using high-speed AFM and neutralizing Hla hemolytic activity with their antisera.

Alpha-toxin (Hla) variants, such as the toxoids HlaH35A, HlaH35L, and HlaH35LH48L, have been shown to lack hemolytic activity and present promising antigen sources for vaccine development against S. aureus. The His35 site is critical in the oligomerization process of Hla during transmembrane pore formation, leading to cell lysis.

Surface display of LukF-PV on spore for oral administration.

Aim: spores are promising candidates for oral vaccine, serving as carriers for foreign proteins from pathogens. Here we utilized CotB, a spore coat protein, to anchor the LukF-PV from onto the surface of spores. Our goal was to generate a new strain and assess the capacity of the recombinant spore strain to induce antibody production in mice.

Effects of Feeding Methionine Hydroxyl Analogue Chelated Zinc, Copper, and Manganese on Growth Performance, Nutrient Digestibility, Mineral Excretion and Welfare Conditions of Broiler Chickens: Part 1: Performance Aspects.

This floor pen study explored the effects of feeding mineral methionine hydroxyl analogue chelates (MMHACs) zinc (Zn), copper (Cu), and manganese (Mn) on growth performance, carcass processing weight and quality, nutrient digestibility, gizzard erosion score, and bone parameters of broilers. One-day-old Ross 308 male chicks ( = 384) were randomly allocated to four dietary treatments with eight replicate pens per treatment and 12 birds per pen. The treatments were as follows: (1) inorganic trace mineral ZnSO 110 ppm, CuSO 16 ppm, MnO 120 ppm (ITM); (2) MMHAC Zn 40 ppm, Cu 10 ppm, Mn 40 ppm (M10); (3) Inorganic trace mineral ZnSO 110 ppm, tribasic copper chloride 125 ppm, MnO 120 ppm (T125); (4) MMHAC Zn 40 ppm, Cu 30 ppm, Mn 40 ppm (M30).

Effects of Feeding Methionine Hydroxyl Analogue Chelated Zinc, Copper, and Manganese on Growth Performance, Nutrient Digestibility, Mineral Excretion, and Welfare Conditions of Broiler Chickens: Part 2: Sustainability and Welfare Aspects.

This study investigated the effects of the dietary supplementation of mineral methionine hydroxyl analogue chelates (MMHACs) zinc (Zn), copper (Cu), and manganese (Mn) on excreta nitrogen and mineral levels, housing conditions, and the welfare status of broilers. Three-hundred eighty-four day-old Ross 308 male chicks were randomly distributed to four dietary treatments, each consisting of eight replicate pens of twelve birds per pen. The treatments were (1) inorganic trace mineral ZnSO (110 ppm), CuSO (16 ppm), and MnO (120 ppm) (ITM); (2) MMHAC Zn (40 ppm), Cu (10 ppm), and Mn (40 ppm) (M10); (3) inorganic trace mineral ZnSO (110 ppm), tribasic copper chloride (125 ppm), and MnO (120 ppm) (T125); and (4) MMHAC Zn (40 ppm), Cu (30 ppm), and Mn (40 ppm) (M30).

In-Plane Anisotropy in van der Waals NiTeSe Ternary Alloy.

The anisotropic properties of materials profoundly influence their electronic, magnetic, optical, and mechanical behaviors and are critical for a wide range of applications. In this study, the anisotropic characteristics of Ni-based van der Waals materials, specifically NiTe and its alloy NiTeSe, utilizing a combination of comprehensive scanning tunneling microscopy (STM), angle-resolved photoemission spectroscopy (ARPES), and density functional theory (DFT) calculations, are explored. Unlike 1T-NiTe, which exhibits trigonal in-plane symmetry, the substitution of Te with Se in NiTe (resulting in the NiTeSe alloy) induces a pronounced in-plane anisotropy.

Complete chloroplast genome sequence of the medicinal plant (Rubiaceae) and phylogenetic analysis.

Lour. 1790, valued for ornamental and medicinal properties, has been extensively utilized in traditional medicinal in Vietnam. This study assembled and characterized the first chloroplast of consisted of 155,333 bp with a GC content of 37.

Fluorescence spectrometric analysis for diagnosing compositional variations in effluent organic matter by chlorination and ozonation.

Analyzing the reactivity of organic matter to oxidants such as chlorination and ozonation is crucial for evaluating the effectiveness of water treatment systems and their potential impacts on environmental safety and human health. This study explored the changes in organic substances, specifically bovine serum albumin (BSA), humic acid sodium salt (HA), and effluent organic matter (EfOM) from a wastewater treatment facility during chlorination and ozonation. Four spectrometric techniques were employed: ultraviolet absorbance at 254 nm (UVA), fluorescent excitation-emission matrix (EEM), synchronous fluorescence two-dimensional correlation spectroscopy (SF-2DCOS), and EEM-parallel factor integrated 2DCOS (EEM-PARAFAC-2DCOS).

Production of Tobacco Etch Virus Protease (TEV) Expressed in the Endotoxin-Free Bacillus subtilis and Its Application.

Tobacco Etch virus (TEV) protease is one of the most common tools for removing fusion tags, but no study has shown that TEV can be expressed at high levels in the GRAS host strain Bacillus subtilis and purified for further application. In this study, the fusion protein BsLysSN-TEV C/S-His-TEV consisting of a fusion tag, N-terminal domain of a lysyl-tRNA synthetase (BsLysSN) coded by B. subtilis lysS gene, placed at the N-terminus followed by an endoprotease TEV cleavage site and then the expression of this fusion protein in the cytoplasm of B.

Comparative genomics revealed new insights into the plastome evolution of (Onagraceae, Myrtales).

The primrose-willow ( L.), a well-defined genus of the Onagraceae family, comprises 87 species widely distributed worldwide. In this study, we sequenced and characterized the complete chloroplast (cp) genomes of three species in the genus, including , , and .

Rescue extracranial vertebral stenting in tandem occlusions: A preliminary Vietnamese study.

Background And Purpose: The acute vertebrobasilar occlusion associated with the poor prognosis, particularly tandem occlusion. However, few data on the efficacy of the endovascular therapy was indicated in this occlusion. We investigated whether the additional rescue extracranial vertebral stenting improved clinical outcome by modified Rankin scale (mRS) score within 3 months after the procedure.

Characterization of the complete chloroplast genome of Lour. 1790 (Helicteriodeae: Malvaceae).

Lour. 1790 is a precious medicinal plant species, especially for treating chronic liver diseases. Genomic data on are limited.

Anti- potential of compounds from sp.: A comprehensive molecular docking and simulation approaches.

In this study, a series of secondary metabolites from Ganoderma sp. were screened against protein targets, including as phosphotransacetylase, clumping factor A, and dihydrofolate reductase, using molecular docking simulations. The chemicals that showed the strongest binding energy with the targeted proteins were ganodermanontriol, lucidumol B, ganoderic acid J, ergosterol, ergosterol peroxide, 7-oxoganoderic acid Z, ganoderic acid AM1, ganosinoside A, ganoderic acid D, and 24-ergosta-7,2-diene-3,5,6-triol.

Rapid identification of SARS-CoV-2 strains via isothermal enzymatic recombinase amplification and nanopore sequencing.

Surveillance of the SARS-CoV-2 genome has become a crucial technique in the management of COVID-19, aiding the pandemic response and supporting effective public health interventions. Typically, whole-genomic sequencing is used along with PCR-based target enrichment techniques to identify SARS-CoV-2 variants, which is a complicated and time-consuming process that requires central laboratory facilities. Thus, there is an urgent need to develop rapid and cost-effective tools for precise on-site detection and identification of SARS-CoV-2 strains.

H3 Acetylation-Induced Basal Progenitor Generation and Neocortex Expansion Depends on the Transcription Factor Pax6.

Enrichment of basal progenitors (BPs) in the developing neocortex is a central driver of cortical enlargement. The transcription factor Pax6 is known as an essential regulator in generation of BPs. H3 lysine 9 acetylation (H3K9ac) has emerged as a crucial epigenetic mechanism that activates the gene expression program required for BP pool amplification.

Analyzing the correlation between protein expression and sequence-related features of mRNA and protein in Escherichia coli K-12 MG1655 model.

It was necessary to have a tool that could predict the amount of protein and optimize the gene sequences to produce recombinant proteins efficiently. The Transim model published by Tuller et al. in 2018 can calculate the translation rate in E.

Development of an Artificial Intelligence Teaching Assistant System for Undergraduate Nursing Students: A Field Testing Study.

Keeping students engaged and motivated during online or class discussion may be challenging. Artificial intelligence has potential to facilitate active learning by enhancing student engagement, motivation, and learning outcomes. The purpose of this study was to develop, test usability of, and explore undergraduate nursing students' perceptions toward the Artificial Intelligence-Teaching Assistant System.

The complete mitochondrial genome of Valenciennes (Siganidae: Acanthuriformes).

Valenciennes 1835 is an essential species for examining reef ecosystems; however, its mitochondrial genome has not been studied. In this research, the mitogenome of was sequenced and characterized. The results revealed a circular genome of 16,505 bp that was composed of A (28.