Surface display of LukF-PV on spore for oral administration.

Aim: spores are promising candidates for oral vaccine, serving as carriers for foreign proteins from pathogens. Here we utilized CotB, a spore coat protein, to anchor the LukF-PV from onto the surface of spores. Our goal was to generate a new strain and assess the capacity of the recombinant spore strain to induce antibody production in mice.

Methods: The fusion sequence of was cloned into and transformed into HT800F. Colony PCR confirmed the generation of the recombinant strain. SporeELISA verified the display of LukF-PV. Mice were orally gavaged with the spore, and the production of IgA antibodies in feces and IgG in blood was evaluated.

Results: We generated a new strain, BsHT2332, that integrated the P into its genome at locus. BsHT2332 successfully displayed LukF-PV on the spore surface. The recombinant spores induced significant IgA and IgG immune responses in mice.

Conclusion: This study demonstrated that spores expressing the antigen LukF-PV can induce an immune response. These findings underscore the potential of the spore platform as a promising approach for vaccine development against .