Antinociceptive and Anti-inflammatory Effect of Nicandrin B Isolated From Datura ferox in Zebrafish.

Nicandrin B (Nic B), a withanolide isolated from Datura ferox leaves, was investigated for its antinociceptive and anti-inflammatory effects in adult zebrafish (Danio rerio). Animals treated with Nic B (4, 20, and 40 mg/kg) showed no toxicity and maintained normal locomotor activity. The compound significantly reduced nociception induced by formalin and hypertonic saline; these effects were reversed by TRPA1 and transient receptor potential vanilloid 1 antagonists, indicating neuromodulation of these targets.

Anthelmintic Potential of Conjugated Long-Chain Fatty Acids Isolated from the Bioluminescent Mushroom .

With praziquantel being the sole available drug for schistosomiasis, identifying novel anthelmintic agents is imperative. A chemical investigation of the fruiting body of the bioluminescent mushroom Berk. resulted in the isolation of new conjugated long-chain fatty acids (8,10,12,13)-12,13-dihydroxy-7-oxo-octadeca-8,10-dienoic acid () and (7,8,9,11)-7,8-dihydroxy-13-oxo-octadeca-9,11-dienoic acid () and three previously described compounds, (7,8,9)-7,8-dihydroxyoctadec-9-enoic acid (), (2)-dec-2-ene-1,10-dioic acid (), and a ketolactone marasmeno-1,15-dione ().

Withanicandrin Isolated from Datura Ferox Promotes Antinociception by Modulating the Asics and TRPS Channels and Anti-Inflammation in Adult Zebrafish.

This is the first study to analyze the anti-inflammatory and antinociceptive effect of withanicandrin, isolated from Datura Ferox leaves, and the possible mechanism of action involved in adult zebrafish (ZFa). To this end, the animals were treated intraperitoneally (i. p.

Petrosamine isolated from marine sponge Petrosia sp. demonstrates protection against neurotoxicity in vitro and in vivo.

According to The World Alzheimer Report 2023 by Alzheimer's Disease International (ADI) estimates that 33 to 38.5 million people worldwide suffer from Alzheimer's Disease (AD). A crucial hallmark associated with this disease is associated with the deficiency of the brain neurotransmitter acetylcholine, due to an affected acetylcholinesterase (AChE) activity.

Tropane alkaloids from the stem bark of Erythroxylum bezerrae.

Six previously undescribed tropane alkaloids, designated as erythrobezerrines A-F, were isolated from the EtOH extract from the stem bark of Erythroxylum bezerrae Plowman. Their structures were elucidated based on the interpretation of the NMR and MS data and in some instances, confirmed by X-ray diffraction analysis. The cytotoxicity of the isolated compounds was evaluated against the cancer cell lines L929, PC-3, HCT-116, SNB-19 and NCI-H460, but only erythrobezerrine C showed moderate activity with IC values of 3.

Chemical constituents and acetylcholinesterase inhibitory activity from the stems of .

Two steroids ( and ), two oxepin derivatives ( and ) and seven flavonoids (-) were isolated from the stems of . Their structures were elucidated on the basis of NMR spectroscopic data, and by comparison with data previously reported in the literature. The ethanol extract from the stems of and the compounds, , , , , and have been evaluated as acetylcholinesterase inhibitors, and among these, the compound exhibited the strongest activity.

Endophytic fungus produces cyclopeptides and plant-related bioactive rotenoids.

In the present study, we integrated liquid chromatography high-resolution mass spectrometry (LC-HRMS) and high-throughput DNA sequencing for prospecting cytotoxic specialized metabolites from , an endophytic fungus associated to the medicinal plant . LC-HRMS profiling allowed identifying putatively eleven compounds in the ethyl acetate extract from broth. Additionally, a chemical fractionation guided by cytotoxicity combined with spectrometric analysis resulted in the isolation of three compounds: the cyclopeptide cyclo-l-Phe-d-Leu-l-Leu-l-Leu-l-lle along with the known rotenoids rotenolone and tephrosin.

Withanolides from leaves of cultivated Acnistus arborescens.

Seven withanolides, including four previously unknown, were isolated from the acetone and ethanol extracts of cultivated specimens of Acnistus arborescens. These four compounds were identified as rel-(18R,22R)-5β,6β:18β,20-diepoxy-3β,18α-dimethoxy-4β-hydroxy-1-oxowith-24-enolide, rel-(20R,22R)-5β,6β-epoxy-4β,16α,20-trihydroxy-1-oxowitha-2,24dienolide, rel-(20R,22R)-16α-acetoxy-6α-chloro-4β,5β,20-trihydroxy-1-oxowitha-2,24-dienolide and rel-(20R,22R)-16α-acetoxy-20-hydroxy-1-oxowitha-2,5,24-trienolide. Their structures were elucidated by interpretation of spectroscopic data (1D and 2D NMR), HRESIMS experiments and comparison with published data for similar compounds.

Cytotoxic Plakortides from the Brazilian Marine Sponge Plakortis angulospiculatus.

Three new plakortides, 7,8-dihydroplakortide E (1), 2, and 10, along with known natural products 3, 4, spongosoritin A (5), 6-8, and plakortide P (9), were isolated from Brazilian specimens of Plakortis angulospiculatus. Compounds 2, 3, 5, and 7-9 displayed cytotoxic activities with IC50 values ranging from 0.2 to 10 μM.

Antiophidic solanidane steroidal alkaloids from Solanum campaniforme.

Three new solanidane alkaloids bearing a 22,23-epoxy ring (1-3) and four known compounds were isolated from leaves of Solanum campaniforme. The structures were determined using spectroscopic techniques, including 1D and 2D NMR, and HRESIMS experiments. The antiophidic activity of the alkaloids was tested against Bothrops pauloensis venom.