Identification, Expression Profiling, Microbial Binding, and Agglutination Analyses of Two Cathepsin B Genes in Black Rockfish ().

As a lysosomal cysteine protease of the papain subfamily, cathepsin B (CTSB) is characterized by its innate immune functions and hydrolytic activity. However, the functions of CTSB in the immune responses of teleosts remain to be clarified. In this study, two CTSB genes in , CTSBa and CTSBb, were identified.

Characterization, expression profiling, and immunological role of Cathepsin D in Sebastes schlegelii during bacterial infection.

Cathepsin D (CTSD), a ubiquitous aspartate hydrolase in eukaryotes, is predominantly localized in lysosomes and involved in the process of substance hydrolysis. While extensive studies have highlighted the importance of CTSD in various physiological and pathological conditions in mammals, its functional roles and mechanisms in fish in responses to bacterial infections remain poorly understood. In this study, two CTSD genes, SsCTSDa and SsCTSDb, were identified in Sebastes schlegelii, and their characteristics were systematically investigated through phylogenetic analysis, syntenic analysis, and tissue-specific expression profiling under both healthy and bacterial infection conditions.

Effects of Environmentally Friendly Aquaculture Chamber Coatings on Enzyme Activities, Histology, and Transcriptome in the Liver of Larimichthys crocea.

Aquaculture vessels have emerged as a sustainable alternative to traditional offshore aquaculture. However, the biological impacts of protective coatings used for vessel interiors are still poorly understood. This study assessed acute stress responses of Larimichthys crocea to epoxy-based aquaculture coatings using actual culture (1-fold) and high-exposure (80-fold) concentrations.

Characterization, immune response and antibacterial mechanism of CXCL12 in black rockfish (Sebastes schlegelii).

Black rockfish (Sebastes schlegelii) is one of the most important mariculture fish in the Western North Pacific. Although the crucial roles of CXCL12 in immune responses against bacterial infection are well-known, the specific mechanisms underlying its involvement in fish immunity remain poorly understood. In this study, we systematically characterized CXCL12a and CXCL12b in Sebastes schlegelii (SsCXCL12a and SsCXCL12b), Firstly, SsCXCL12a/b were ubiquitously expressed across all seven tissues.