Hybrid lipidic and fluorinated polymer microbubbles for blood-brain barrier opening: a comparative study with SonoVue.

Microbubble-mediated focused ultrasound is a promising strategy for transient and localized blood-brain barrier (BBB) permeabilization, enabling drug delivery to the brain. Optimizing microbubble stability and acoustic response is essential to maximize treatment efficiency and minimize potential damage. This study introduces an innovative microbubble formulation with a phospholipid-fluoropolymer shell (LIP-POL), designed to enhance circulation persistence while maintaining a low cavitation threshold.

Using eDNA tools to examine the impact of kelp farming on underlying sediments.

Using environmental DNA (eDNA)-based tools, we examined sediments underlying a ~ 1.25 hectare commercial kelp farm in the Gulf of Maine growing sugar kelp (Saccharina latissima) for two farming seasons, post-harvest. Two eDNA methods were used: a newly designed S.

Posttraumatic Growth in Intensive Care Unit Health Care Professionals After COVID-19.

Importance: Posttraumatic growth (PTG) refers to positive psychological changes following adversity, including deeper relationships and a greater appreciation for life.

Objective: To assess PTG among intensive care unit (ICU) health care professionals 4 years after the COVID-19 pandemic and explore its association with resilience, anxiety, and depression.

Design, Setting, And Participants: This cross-sectional study invited ICU health care professionals (nursing staff, medical staff [residents, interns, clinical fellows, and senior intensivists], and other professionals providing patient care) in ICUs in general or university-affiliated hospitals in France and Belgium to complete online questionnaires between March 15 and May 15, 2024.

Deep-learning recognition and tracking of individual nanotubes in low-contrast microscopy videos.

This study addresses the challenge of analyzing the growth kinetics of carbon nanotubes using in situ homodyne polarization microscopy (HPM) by developing an automated deep learning (DL) approach. A Mask-RCNN architecture, enhanced with a ResNet-FPN backbone, was employed to recognize and track individual nanotubes in microscopy videos, significantly improving the efficiency and reproducibility of kinetic data extraction. The method involves a series of video processing steps to enhance contrast and used differential treatment techniques to manage low signal and fast kinetics.

Mitochondrial genome copy number variation across tissues in mice and humans.

The mammalian mitochondrial genome (mtDNA) is multicopy and its copy number (mtCN) varies widely across tissues, in development and in disease. Here, we systematically catalog this variation by assaying mtCN in 52 human tissues across 952 donors (10,499 samples from the Genotype-Tissue Expression project) and 20 murine tissues using qPCR, capturing 50- and 200-fold variation, respectively. We also estimate per cell mtCN across 173 human cell lines from the Cancer Cell Line Encyclopedia using whole-genome sequencing data and observe >50-fold variation.

Tracking wild-type measles virus in wastewater using multiplex RT-dPCR, A novel tool for measles surveillance.

A multiplex digital RT-PCR (RT-dPCR) assay targeting three distinct regions of the measles virus genome was developed for wastewater surveillance. This method was applied to 40 mL samples collected at the inlets of two French wastewater treatment plants located in urban areas where clinical measles cases involving genotypes B3 and D8 had been reported. Detection was performed retrospectively on weekly samples collected between January and July 2024 as part of the routine national SARS CoV-2 wastewater surveillance network.

Detection of abortifacient agents in domestic ruminants, with a specific focus on .

Q fever is a widespread zoonotic disease caused by the bacterium, . In ruminants, can cause abortions, stillbirths, premature births, and weak offspring. As part of the EU-funded Q-Net-Assess International Coordination of Research on Infectious Animal Diseases (ICRAD)-project, aimed at generating the most comprehensive understanding of genetic variation to date and determining the implications of this genetic variation for zoonotic risk, pathogenicity and control of infection, we have reviewed the protocols reported by the different project partners and/or countries to diagnose abortion in domestic ruminants.

Photodynamic drug delivery for cancer therapy: Designing liposomes for light-controlled release and enhanced drug efficacy.

Photodynamics involves the use of photocatalytic compounds that, upon excitation with light, produce reactive oxygen species and have seen widespread applications in the treatment of cancer in the form of photodynamic therapy. Within the field of drug delivery, photodynamics has emerged as a strikingly effective approach for spatiotemporal-controlled drug release by harnessing photochemical redox reactions to destabilize lipid nanoformulations that contain oxidation-susceptible excipients. Despite highly promising outcomes in preclinical models, such controlled release modalities have not yet been explored in clinical cancer trials.

An updated inventory of genes essential for oxidative phosphorylation identifies a mitochondrial origin in familial Ménière's disease.

Mitochondrial disorders (MDs) are among the most common inborn errors of metabolism, and dysfunction in oxidative phosphorylation (OXPHOS) is a hallmark. Their complex mode of inheritance and diverse clinical presentations render the diagnosis of MDs challenging, and, to date, most lack a cure. Here, we build on previous efforts to identify genes necessary for OXPHOS and report a highly complementary galactose-sensitized CRISPR-Cas9 "growth" screen, presenting an updated inventory of 481 OXPHOS genes, including 157 linked to MDs.

Inferring human behavior through online social networks may provide accurate behavioral estimates for outbreak forecasting of arboviruses.

Human behavior is known to be a fundamental, yet often neglected, component of infectious disease epidemiology, especially during outbreaks. To quantify its role and fluctuations, analyzing message contents on popular online social networks - part of so-called digital epidemiology - is a promising approach. However, such methods could be biased and generate estimation errors since social media users may not be representative of the general population.

A Water Stable U(IV) Complex Based on a Macrocyclic Ligand Displaying NIR Emission.

Complexation of U(IV) by encapsulation into the cavity of a 1,4,7-triazacyclonane (tacn) macrocycle functionalized by phosphonated pyridyl arms affords a water-stable and soluble [U(IV)L] complex. The coordination process was monitored by electronic UV-Vis-NIR absorption spectroscopy and the corresponding complex was characterized by H-, C- and P-NMR spectroscopy and by mass spectrometry. Density functional theory (DFT) modeling shows the cation to be embedded into the cavity of the nonadentate ligand, as confirmed by analysis of the paramagnetic contributions of U(IV) on the chemical shifts of the proton nuclei.

POLR1A inhibits ferroptosis by regulating TFAM-mediated mitophagy and iron homeostasis.

Evasion of programmed cell death (PCD) is a hallmark of cancer, yet the mechanisms underlying resistance to ferroptosis - an iron-dependent form of PCD triggered by excessive lipid peroxidation - remain incompletely understood. Here, we identify a previously unrecognized nucleolar-mitochondrial signaling axis that promotes ferroptosis resistance in pleural mesothelioma (PM) and potentially other cancers. This pathway involves RNA polymerase I (PolI) catalytic subunit A (POLR1A) and mitochondrial transcription factor A (TFAM), which together regulate mitophagy and intracellular iron metabolism to suppress ferroptosis.

Renal replacement therapy in an intensive care unit: guidelines from the SRLF-GFRUP consensus conference.

Background: Although largely used, the place of extracorporeal renal replacement therapy (RRT) in acute kidney injury (AKI) in intensive care unit (ICU) patients has yet to be clarified. The French Intensive Care Society (Société de Réanimation de Langue Française, SRLF) and the French Pediatric Group of Intensive Care and Emergency (Groupe Francophone de Réanimation et d'Urgence Pédiatrique, GFRUP) organized a consensus conference in November 2024.

Methods: A committee, without any conflict of interest (CoI) on the subject, defined seven generic questions and drew up a list of sub questions according to the population, intervention, comparison and outcomes (PICO) model.

Spatially coordinated predation with division of labor increases feeding success in killer whales.

Group hunting is widespread among large vertebrates and is known to confer considerable advantages compared with foraging alone. Yet, the mechanisms underlying group hunting, including how social predators are organized during a hunt, are largely unknown for marine predators. Using drone videos, we tracked the predatory behavior of killer whales along the Norwegian coast to test the hypothesis that group hunting is organized in space, both in terms of individual roles and interactions with conspecifics.

Vasectomy Procedures on the Rise in France From 2010 to 2022.

Context: While the annual rate of vasectomy has dropped worldwide, we have little knowledge of its prevalence in France.

Objective: To describe the use of vasectomy in France from 2010 to 2022.

Methods: This population-based study relied on the French national health data system (SNDS, 99.

Trame: Platform Ubiquitous, Scalable Integration Framework for Visual Analytics.

Trame is an open-source, Python-based, scalable integration framework for visual analytics. It is the culmination of decades of work-by a large and active community-beginning with the creation of VTK, the growth of ParaView as a premier high-performance, client-server computing system, and more recently the creation of web tools, such as VTK.js and VTK.

Designing synthetic microbial communities for enhanced anaerobic waste treatment.

Synthetic microbial communities (SynComs) are powerful tools for investigating microbial interactions and community assembly by focusing on minimal yet functionally representative members. Here, we will highlight key principles for designing SynComs, specifically emphasizing the anaerobic digestion (AD) microbiome for waste treatment and upcycling. The AD process has traditionally been used to reduce organic waste volume while producing biogas as a renewable energy source.

Machine learning to optimize use of natriuretic peptides in the diagnosis of acute heart failure.

Aims: B-type natriuretic peptide (BNP) and mid-regional pro-atrial natriuretic peptide (MR-proANP) testing are guideline-recommended to aid in the diagnosis of acute heart failure. Nevertheless, the diagnostic performance of these biomarkers is uncertain.

Methods And Results: We performed a systematic review and individual patient-level data meta-analysis to evaluate the diagnostic performance of BNP and MR-proANP.

Uridine-sensitized screening identifies genes and metabolic regulators of nucleotide synthesis.

Nucleotides are essential for nucleic acid synthesis, signaling, and metabolism, and can be synthesized or through salvage. Rapidly proliferating cells require large amounts of nucleotides, making nucleotide metabolism a widely exploited target for cancer therapy. However, resistance frequently emerges, highlighting the need for a deeper understanding of nucleotide regulation.

Harmonisation of the diagnostic performances of serological ELISA tests for C. burnetii in ruminants in the absence of a gold standard: Optimal cut-offs and performances reassessment.

None of the three ELISA tests currently available in Europe for the serological diagnosis of Coxiella burnetii (C. burnetii) infections in ruminants can be considered as a gold standard. The difference in their diagnostic performances (e.

Patient experience and primary care teams: a cross-sectional survey of French elderly patients.

Objective: To assess whether patient experience is better for patients followed in a primary care team (PCT) than for patients with traditional follow-up in usual care.

Design: A cross-sectional survey based on a self-administered questionnaire.

Setting: Pays de la Loire geographical area (located on the French west coast).

Quantitative vessel mapping on increment cores: a critical comparison of image acquisition methods.

Introduction: Quantitative wood anatomy is critical for establishing climate reconstruction proxies, understanding tree hydraulics, and quantifying carbon allocation. Its accuracy depends upon the image acquisition methods, which allows for the identification of the number and dimensions of vessels, fibres, and tracheids within a tree ring. Angiosperm wood is analysed with a variety of different image acquisition methods, including surface pictures, wood anatomical micro-sections, or X-ray computed micro-tomography.

Wastewater-Based Epidemiological Surveillance in France: The SUM'EAU Network.

Wastewater surveillance is a powerful public health tool which gained global prominence during the COVID-19 pandemic. This article describes the development and implementation of the national wastewater surveillance network in France: SUM'EAU. Preliminary work included defining a sampling strategy, evaluating/optimising analytical methods, launching a call for tenders to select network laboratories and producing wastewater monitoring indicators.