TYROBP overexpression alters macrophage phenotype and enhances pancreatic cancer stemness through STAT3 and PKM2 signaling.

Background: Pancreatic ductal adenocarcinoma (PDAC) is characterized by a complex tumor microenvironment (TME) that influences tumor progression and therapeutic responses. TYRO protein tyrosine kinase binding protein (TYROBP), a transmembrane polypeptide involved in immune cell signaling, has been implicated in PDAC and associated with M2 macrophage polarization.

Methods: We investigated the correlation between TYROBP expression and M2 macrophage infiltration in PDAC tissues using multiplex immunofluorescence staining.

NFATC2/SERPINE1/JAK3/STAT3 signaling feedback loop in gastric cancer: immune evasion and anti-PD-1 resistance.

Objectives: This research investigates how the SERPINE1-associated tumor microenvironment influences anti-PD-1 treatment response in gastric carcinoma (GC).

Methods: Bioinformatics analysis, cellular experiments, and animal models were employed to quantify the levels of NFATC2, SERPINE1, JAK3, STAT3, and to explore their associations with various biological behaviors of GC cells, encompassing proliferation, migration, invasiveness, EMT, and immune cell infiltration. Additionally, by constructing a GC tumor-bearing model, we assessed the efficacy of knocking down SERPINE1 in combination with anti-PD-1 therapy.

The miR-146a-associated HDAC2 regulation of PI3K is involved in pancreatitis in vitro.

Purpose: To investigate the association between miR-146a/HDAC2 and their regulatory roles on the PI3K expression during pancreatitis.

Methods: Rat pancreatic AR42J cells were treated with LPS for simulating pancreatitis. Expression levels of inflammatory factors (IL-6, TNF-α) and miR-146a were d determine the optimal LPS concentration for establishing an in vitro pancreatitis model Cell proliferation and apoptosis were analyzed using CCK-8 and flow cytometry Immunofluorescence was performed to assess co-localization of HDAC2 and PI3K.

The Mechanism of HDAC2 Inhibitors on Chronic Pancreatitis Pain.

Background: Chronic pancreatitis (CP) is marked by persistent inflammation and fibrosis of the pancreas, often causing severe abdominal pain. The pain mechanism involves complex interactions between pancreatic inflammation and spinal nerve activity. Histone deacetylase 2 (HDAC2) is implicated in neural processes and pain modulation, making it a potential target for CP pain management.

TYROBP promotes the spread of pancreatic cancer by causing M2 TAM polarization.

Background And Aim: M2-polarized tumor-associated macrophages (M2 TAMs) are known to promote cancer progression, and exosomes are crucial mediators of communication within the tumor microenvironment (TME). However, the specific role of exosomes derived from M2 TAMs in pancreatic cancer (PC) progression remains poorly understood. Tyrosine kinase binding protein (TYROBP, also known as DAP12 for DNAX activating protein-12) is a transmembrane signal transduction polypeptide that interacts with immune cell receptors, influencing cellular functions via signal transduction pathways.

Effect of digital three-dimensional reconstruction technique combined with indocyanine green (ICG) excretion test for precision hepatectomy in primary liver cancer.

Objective: The aim of this study was to evaluate the residual volume of liver reserve function in liver cancer patients using three-dimensional reconstruction technique (3D technology) and the indocyanine green (ICG) excretion test.

Methods: A retrospective analysis was conducted, and data were collected from 90 liver cancer patients in Ganzhou People's Hospital between January 2017 and December 2021. The control group underwent preoperative resectability evaluation based on traditional two-dimensional images, whereas the experimental group underwent digital three-dimensional reconstruction technique combined with indocyanine green (ICG) excretion test.

Determination of the Mono and Dibromo Derivatives Ratio Resulting from Semiconductor Bromination Using Ultraviolet-visible Absorption Spectroscopy and Gaussian Peak Fitting.

The chemical-industrial production of organic semiconductors urgently needs a cheap and fast approach to determine the components' proportion of the reaction system. In the present work, the Gaussian peak fitting method was applied to process monobromo and dibromo-substituted perylene diimide mixed solutions' ultraviolet-visible absorption curves. The functional relationship formula between the peak-intensity ratio and the component ratio is then concluded.

[Discussion of 2011 Tokyo Declaration on Japanese Acupuncture and Moxibustion].

The contents of 2011 Tokyo Declaration on Japanese Acupuncture and Moxibustion (Declaration for short) and its effect on development situation, current status, features, opportunities and challenges of Japanese acupuncture and moxibustion are introduced, some proposals brought up in the Declaration are analyzed as well. The Declaration summarizes six characteristics of Japanese acupuncture and moxibustion, including paying great attention to palpation techniques such as pulse and abdominal diagnosis, always selecting response point in the meridian during acupuncture treatment, etc. Also six proposals have been brought up to promote the development of Japanese acupuncture and moxibustion, such as being devoted to spread the latest knowledge of acupuncture and moxibustion to medical professionals and the public in order to get correct understanding and proper evaluation, etc.

[Affection of acupotomy lysis on leu-enkephalin (L-ENK) content in different parts of centrum of rats with knee osteoarthritis].

Objective: To study the analgesia mechanism of needle-knife lysis in spinal cord and other parts of central nervous system by comparing the changes of Leu-Enkephalin (L-ENK) content in different parts of centrium of rats undergone needle-knife lysis and electro-acupuncture respectively.

Methods: Sixty healthy SD rats were randomly devided into normal control group, model group, needle-knife lysis (NKL) group and electro-acupuncture (EA) group. 4% papain solution mixed with 0.