Recent advances of small-molecule fluorescent probes for sensing and imaging biomarkers of experimental intrinsic drug-induced liver injury.

Drug-induced liver injury (DILI) is a disorder identified by hepatic damage resulting from pharmaceutical drugs, affecting hepatocytes, bile duct cells, and vascular endothelial cells, which leads to variable degrees of inflammation and functional impairment. The diagnosis of DILI predominantly depends on a history of exposure to potential medicines, the elimination of alternative liver disorders, or conventional biomarkers such as alanine aminotransferase (ALT) and aspartate aminotransferase (AST). However, the lack of precise, objective diagnostic markers and effective therapeutic strategies continues to hinder progress in this field.

Single-cell sequencing and spatial transcriptomics reveal the evolution of glucose metabolism in hepatocellular carcinoma and identify G6PD as a potential therapeutic target.

Background: Glucose metabolism reprogramming provides significant insights into the development and progression of malignant tumors. This study aims to explore the temporal-spatial evolution of the glucose metabolism in HCC using single-cell sequencing and spatial transcriptomics (ST), and validates G6PD as a potential therapeutic target for HCC.

Methods: We collected single-cell sequencing data from 7 HCC and adjacent non-cancerous tissues from the GSE149614 database, and ST data from 4 HCC tissues from the HRA000437 database.

Precision Imaging of Biothiols in Live Cells and Treatment Evaluation during the Development of Liver Injury via a Near-Infrared Fluorescent Probe.

In this study, a biothiol-sensitive near-infrared (NIR) fluorescent sensor, BDP-CYS, based on a coumarin-hemicyanine skeleton, was designed and developed based on thiol-halogen SAr nucleophilic substitution. BDP-CYS was successfully implemented to ratiometrically monitor endogenous and exogenous Cys, Hcy, and GSH in living cells as well as to distinguish between normal and cancer cells. Furthermore, the probe was utilized to detect changes of biothiols in drug-induced hepatotoxicity and evaluate the treatment effectiveness of diabetes-associated liver injury in vivo.

A nomogram model for predicting intramyocardial hemorrhage post-PCI based on SYNTAX score and clinical features.

Objective: The aim of this study is to develop a nomogram model for predicting the occurrence of intramyocardial hemorrhage (IMH) in patients with Acute Myocardial Infarction (AMI) following Percutaneous Coronary Intervention (PCI). The model is constructed utilizing clinical data and the SYNTAX Score (SS), and its predictive value is thoroughly evaluated.

Methods: A retrospective study was conducted, including 216 patients with AMI who underwent Cardiac Magnetic Resonance (CMR) within a week post-PCI.

A novel natural killer-related signature to effectively predict prognosis in hepatocellular carcinoma.

Background: Hepatocellular carcinoma (HCC) is a prevalent tumor that poses a significant threat to human health, with 80% of cases being primary HCC. At present, Early diagnosis and predict prognosis of HCC is challenging and the it is characterized by a high degree of invasiveness, both of which negatively impact patient prognosis. Natural killer cells (NK) play an important role in the development, diagnosis and prognosis of malignant tumors.