Senolytic-Resistant Senescent Cells Have a Distinct SASP Profile and Functional Impact: The Path to Developing Senosensitizers.

The senescent cell (SC) fate is linked to aging, multiple disorders and diseases, and physical dysfunction. Senolytics, agents that selectively eliminate 30-70% of SCs, act by transiently disabling the senescent cell anti-apoptotic pathways (SCAPs), which defend those SCs that are pro-apoptotic and pro-inflammatory from their own senescence-associated secretory phenotype (SASP). Consistent with this, a JAK/STAT inhibitor, Ruxolitinib, which attenuates the pro-inflammatory SASP of senescent human preadipocytes, caused them to become "senolytic-resistant".

Patient Room Design: Engaging Clinical Teams Through Iterative Simulation-Based Evaluation.

This study evaluated a standardized patient room designed for multiple acuity levels in a quaternary care academic medical center by actively engaging clinical teams through an iterative simulation-based evaluation process using physical mock-ups. Patient rooms are replicated multiple times in hospitals, and their design impacts patient and staff outcomes. Balancing often-conflicting needs and designing for future flexibility can be challenging.

Associations among Human Milk Polyunsaturated Fatty Acids and Infant Sleep Patterns: A Cross-Sectional Study.

Background: Infant sleep is critical for cognitive, emotional, and long-term health outcomes. Although diet-sleep relationships are established, limited research has explored how polyunsaturated fatty acids (PUFAs) in human milk (HM) relate to infant sleep.

Objectives: This study aims to examine associations between PUFAs in HM and sleep patterns in 2-mo-old infants exclusively fed with HM.

"Shaking the ladder" reveals how analytic choices can influence associations in nutrition epidemiology: beef intake and coronary heart disease as a case study.

Nutrition epidemiological models involve many analytic decisions, such as defining exposures, selecting which covariates to include, or configuring variables in different ways. We explored the impact of analytical decisions on conclusions in nutrition epidemiology using self-reported beef intake and incident coronary heart disease as a case study. We used REasons for Geographic and Racial Differences in Stroke (REGARDS) data, and selected covariates and their configurations from published literature to recapitulate common models used to assess associations between meat intake and health outcomes.

Food noise: definition, measurement, and future research directions.

The term food noise has been recently popularized by individuals managing their weight or adhering to specific diets for health reasons. Anecdotal evidence from patients and clinical observations suggests that food noise involves constant preoccupation with food-related decisions-such as which foods to eat, caloric intake, macronutrient balance, and meal timing-which can become intrusive and unpleasant. Food noise also appears to affect cognitive burden and quality of life, and is being cited as one reason weight-loss attempts fail.

Effectiveness of a digital weight management program on diet quality: a randomized controlled trial.

Background: The effect of behavioral commercial weight programs (CP) on weight loss is clear, yet their effects on diet quality are less studied.

Objectives: This study aims to evaluate the relative effectiveness of a digital CP on diet quality compared with standard nutritional guidance (SNG) over 6 months.

Methods: This randomized controlled trial (clinicaltrials.

BAHCC1 binds H4K20me1 to facilitate the MCM complex loading and DNA replication.

Mono-methylation of histone H4 lysine 20 (H4K20me1) regulates DNA replication, cell cycle progression and DNA damage repair. How exactly H4K20me1 regulates these biological processes remains unclear. Here, we report that an evolutionarily conserved tandem Tudor domain (TTD) in BAHCC1 (BAHCC1) selectively reads H4K20me1 for facilitating replication origin activation and DNA replication.

Adjusting for covariates representing potential confounders, mediators, or competing predictors in the presence of measurement error: Dispelling a potential misapprehension and insights for optimal study design with nutritional epidemiology examples.

Background: Variables such as dietary intake are measured with error yet frequently used in observational epidemiology. Although this limitation is sometimes noted, these variables are still often modeled as covariates without formal correction or sincere dialogue about measurement unreliability potentially weakening the validity of statistical conclusions. Further, larger sample sizes increase power (bias) to detect spurious correlations.

"Non-Markovian" and "directional" errors inhibit scientific self-correction and can lead fields of study astray: an illustration using gardening and obesity-related outcomes.

Herein we coin the terms non-Markovian error and biased Markovian error to describe categories of scientific errors for which general progress within a field of study may be unlikely to result in scientific self-correction. We provide examples of such errors and show their impact on studies about gardening and obesity.

The harms of high protein intake: conjectured, postulated, claimed, and presumed, but shown?

High-protein (HP) diets and protein-supplemented foods and beverages have become increasingly popular in adults due to potential benefits relating to appetite, energy intake, body weight, and body composition, and questions have been posed regarding whether current dietary recommendations for protein are too low. At the same time, health concerns relating to high-protein diets have been widespread in the literature for >60 y. However, the conjectured harms of HP diets, which remain prevalent in the lay and sometimes academic literature, are often without strong scientific evidence or may actually be contradicted to a reasonable degree of certainty by scientific evidence.

Fecal glucocorticoid metabolite and T3 profiles of orphaned elephants differ from non-orphaned elephants in Zambia.

Background: Elephants provide valuable insight into how early-life adverse events (ELAEs) associate with animal health and welfare because they can live to advanced ages, display extensive cognitive and memory capabilities, and rely heavily on social bonds. Although it is known that African savanna elephants that experienced ELAEs, such as being orphaned due to human activities, have altered behavioral outcomes, little is known regarding the physiological consequences associated with those stressors.

Methods: We compared fecal glucocorticoid (fGCM) and thyroid (fT3) metabolites as well as body condition scores (BCS) in rescued and rehabilitated orphaned (early-dry season:  = 20; late-dry season:  = 21 elephants) African savanna elephants in Kafue National Park, Zambia to age- and sex-matched wild non-orphaned controls groups (early-dry season:  = 57; late-dry season:  = 22 elephants) during the early- (May/June) and late- (September/October) dry seasons, respectively.

Improving Statistical Rigor in Animal Aging Research by Addressing Clustering and Nesting Effects: Illustration with the National Institute on Aging's Intervention Testing Program Data.

Clustering effects, such as those introduced by housing animals in shared cages, are often overlooked in preclinical lifespan studies, despite their potential to distort variance estimates and inflate Type I error rates, leading to misleading conclusions. This methodological oversight reduces statistical rigor and may undermine the reliability of findings. To address this gap, the current study examines the impact of accounting for clustering and nesting effects on lifespan analyses by comparing the results of statistical models which both account for and ignore these effects.

Using patient journey mapping and provider workflows to understand process barriers to pediatric mental and behavioral health care in emergency departments.

With the rise in mental and behavioral health (MBH) conditions among school-age children in the United States (US), the number of Emergency Department (ED) visits have also increased. However, ED settings struggle to meet the needs of children with MBH conditions safely and efficiently. This study integrated SEIPS (Systems Engineering Initiative for Patient Safety) 3.

Leukocyte poor platelet rich plasma vs leukocyte rich platelet rich plasma as a treatment for cervical facetogenic pain: A pooled analysis.

Background: This study aimed to compare the effectiveness and safety of leukocyte-poor platelet-rich plasma (LP-PRP) and leukocyte-rich platelet-rich plasma (LR-PRP) for the treatment of cervical facetogenic pain through a pooled analysis of two independent studies. The goal was to provide preliminary evidence comparing the effect on pain relief and functional improvement over time to help inform future research.

Methods: The pooled analysis integrated data from two studies: a prospective case series of LR-PRP (n = 36) and a randomized controlled trial (RCT) comparing LP-PRP (n = 21) with corticosteroid injections.

Proposing Design Evaluation Metrics for Anesthesia Providers' Workspace in Ambulatory Surgical Settings.

This paper describes the development and utilization of two design evaluation metrics aimed to measure design performance for improving circulation and workflow, situational awareness, and visibility for medication-related activities for anesthesia providers' within their workspace in operating rooms. Proactive performance evaluation of the design of critical areas such as operating rooms can help improve the safety of patients and staff workflows. This paper builds on previous work on task-switching behaviors in anesthesia workspaces to develop performance-based design evaluation metrics for anesthesia providers' workspaces based on their tasks performed during the patient preparation, intra-operative, and post-operative phases of the surgery, considering the presence of multiple anesthesia providers.

The misalignment of incentives in academic publishing and implications for journal reform.

For most researchers, academic publishing serves two goals that are often misaligned-knowledge dissemination and establishing scientific credentials. While both goals can encourage research with significant depth and scope, the latter can also pressure scholars to maximize publication metrics. Commercial publishing companies have capitalized on the centrality of publishing to the scientific enterprises of knowledge dissemination and academic recognition to extract large profits from academia by leveraging unpaid services from reviewers, creating financial barriers to research dissemination, and imposing substantial fees for open access.

Evaluating the Reproducibility and Verifiability of Nutrition Research: A Case Study of Studies Assessing the Relationship Between Potatoes and Colorectal Cancer.

Background: The credibility of nutritional research is dependent on the rigor with which studies are conducted and the ability for independent assessment to be performed. Despite the importance of these, more work is needed in the field of nutrition to buttress the trustworthiness of nutrition research.

Objective: To develop and apply a process for evaluating the rigor, reproducibility, and verifiability of nutritional research, using the relationship between potato consumption and Colorectal cancer (CRC) as a case study.

Intra-articular corticosteroid injections versus platelet-rich plasma as a treatment for cervical facetogenic pain: a randomized clinical trial.

Objective: The study's primary objective was to compare the effectiveness of intra-articular platelet-rich plasma injections versus corticosteroid injections for the treatment of cervical facetogenic pain. Secondary aims were to compare self-rated disability, pain self-efficacy, and the safety of the procedure between groups.

Methods: A single-site randomized double-blind controlled trial with 40 participants assigned to receive either leucocyte-poor, low-concentrate platelet-rich plasma injections or corticosteroid injection without local anesthetic into the cervical facet joint under fluoroscopy.

A hematology-based clock derived from the Study of Longitudinal Aging in Mice to estimate biological age.

Biological clocks and other molecular biomarkers of aging are difficult to implement widely in a clinical setting. In this study, we used routinely collected hematological markers to develop an aging clock to predict blood age and determine whether the difference between predicted age and chronologic age (aging gap) is associated with advanced aging in mice. Data from 2,562 mice of both sexes and three strains were drawn from two longitudinal studies of aging.

Hidden: A Baker's Dozen Ways in Which Research Reporting is Less Transparent than it Could be and Suggestions for Implementing Einstein's Dictum.

The tutelage of our mentors as scientists included the analogy that writing a good scientific paper was an exercise in storytelling that omitted unessential details that did not move the story forward or that detracted from the overall message. However, the advice to not get lost in the details had an important flaw. In science, it is the many details of the data themselves and the methods used to generate and analyze them that give conclusions their probative meaning.

Reproduction has immediate effects on female mortality, but no discernible lasting physiological impacts: A test of the disposable soma theory.

The disposable soma theory (DST) posits that organisms age and die because of a direct trade-off in resource allocation between reproduction and somatic maintenance. DST predicts that investments in reproduction accentuate somatic damage which increase senescence and shortens lifespan. Here, we directly tested DST predictions in breeding and nonbreeding female C57BL/6J mice.

Mapping epidermal and dermal cellular senescence in human skin aging.

Single-cell RNA sequencing and spatial transcriptomics enable unprecedented insight into cellular and molecular pathways implicated in human skin aging and regeneration. Senescent cells are individual cells that are irreversibly cell cycle arrested and can accumulate across the human lifespan due to cell-intrinsic and -extrinsic stressors. With an atlas of single-cell RNA-sequencing and spatial transcriptomics, epidermal and dermal senescence and its effects were investigated, with a focus on melanocytes and fibroblasts.