Downregulated miR-199a-3p in Preeclamptic Placenta-derived Exosomes from Cord Blood Hinders VEGF-induced Fetal Glomerular Dysplasia Through Inhibiting Akt1(S473) Phosphorylation via Targeting PHLPP2.

Background: Preeclampsia is amultisystem disorder involving in inflammatory responses and metabolic dysfunction of maternal-fetal circulation. Recently, researchers found it threatens renal health of offspring in adulthood. Growing evidence indicated chronic kidney disease is associated with glomeruli deficiencies during intrauterine development.

Mechanism of lidocaine-induced ROS generation triggering DNA double-strand breaks and promoting intervertebral disc cell senescence via the MYC-DUSP1-P53 axis.

Discogenic lower back pain (DLBP) is a prevalent lumbar disorder. Functional Anesthetic Discography (FAD) is the primary diagnostic method for DLBP, with a high positivity rate. However, the mechanism by which lidocaine, a local anesthetic commonly used in FAD, induces damage to intervertebral disc cells remains unclear.

Unveiling the Failure Mechanism of Pt Coating on Ti Porous Transport Layer in Simulated PEMEC Environment.

Pt coating has been considered a promising strategy to protect the Ti porous transport layer substrate in a proton exchange membrane electrolyzer from corrosion. However, despite significant advancements in understanding the composition, process, and structure of Pt coatings, the underlying causes of coating failure remains unclear. Here, we systematically investigate the failure processes of magnetron-sputtered Pt coatings on Ti substrates through ex situ electrochemical tests that simulate anode operational conditions.

Immune-Modulating Dual-Targeted Nanomaterials for Low-Temperature Photothermal-Photodynamic-Chemodynamic Therapy of Osteosarcoma Targeting Tumor and Endothelial Cells.

Osteosarcoma is a highly aggressive bone tumor with limited treatment options because of its drug resistance and tumor heterogeneity. In this study, we developed a multifunctional nanomaterial, P-FeO@Pal@HM, combining porous FeO nanoparticles, the DNA intercalator palmatine, and a hybrid membrane coating derived from osteosarcoma cells and tumor-associated endothelial cells. The FeO core facilitates a Fenton-like reaction, generating reactive oxygen species (ROS) to enhance DNA damage, whereas palmatine (Pal) inhibits RRM2 (ribonucleotide reductase regulatory subunit M2) expression, blocking DNA repair and inducing apoptosis.

Immune Signatures Distinguish Pure and Mixed Sepsis in Critical COVID-19: A Retrospective Cohort Study.

Background: COVID-19-associated sepsis poses unique challenges in intensive care units (ICUs), where patients are often immunocompromised and prone to secondary bacterial infections. Differentiating immune phenotypes between pure viral and viral-bacterial sepsis is essential for timely diagnosis and personalized treatment.

Objective: To compare clinical characteristics, immune profiles, and outcomes between patients with pure COVID-19 sepsis and those complicated by confirmed secondary bacterial infection, and to identify immune markers capable of differentiating sepsis phenotypes.