Short-term moderate-intensity treadmill running may confer a greater benefit to bone strength than high-intensity treadmill running in 12-month-old C57BL/6 mice.

Ageing is linked to pathological changes in bone structure and the loss of bone mass and strength. Exercise is a non-pharmacological intervention that may improve bone mass; however, the effects on bone strength, structure, and material properties remain unclear. We tested the effects of work-matched moderate- and high-intensity treadmill exercise on bone structure and strength in the mature (middle-aged) murine skeleton.

Elemental mass spectrometry imaging of biomolecules using metal-conjugated probes.

In situ imaging of proteins, RNA, immune cells and other biomolecules is necessary to determine their function, interactions and roles in disease pathology. Increasingly, this is achieved via metal-conjugated probes in conjunction with elemental mass spectrometry imaging (MSI). This targeted technique is capable of simultaneously imaging up to 40 analytes, in comparison to the traditional bioimaging techniques that use fluorescent or chromogenic reagents that are typically restricted to less than four analytes without complex sample handling and analysis workflows.

The effect of rotenone contamination on high-resolution mitochondrial respiration experiments.

Background: High-resolution respirometry is commonly used in skeletal muscle research and exercise science to measure mitochondrial respiratory function in both permeabilized muscle fibers and isolated mitochondria. Due to the low throughput and high cost of the most used respirometer, the Oroboros 2k (O2k), multiple experiments are often conducted within the same chamber in short succession. Despite this, no methodological consideration has been given for the potential contamination of inhibitors, used to investigate the contribution of specific complexes within the electron transport chain, between experiments.

Bikeability cycle training: a route to increasing young people's subjective wellbeing? A retrospective cohort study.

Introduction: Increasing the population's subjective wellbeing is an explicit aim of current UK government policies. The wellbeing of children and young people in the UK is deteriorating, and less than half of them meet national physical activity guidelines, despite the demonstrable benefits of physical activity for wellbeing. Hence, it is important to identify economically viable and effective public health interventions to increase young people's physical activity, and consequently, their wellbeing.

Exploring the link between the risk of violent injury in adolescents and historic redlining practices.

Violent injuries tend to cluster together geospatially. The discriminatory housing practice of redlining undertaken by the United States federal government in the 1930s has been repeatedly linked with various contemporary community-level disparities. However, no known work has explored the association between historical redlining and the risk of violent injuries among adolescents.