Phytomodulatory latex proteins improve blood homeostasis in diabetic mice with disseminated bacterial infection.

Blood rheology and hematology are essential factors in vascular health in diabetic patients. Additionally, in patients with systemic infections, blood characteristics change rapidly, increasing cardiovascular risk. The present study examined the role of immunomodulatory proteins from Calotropis procera latex (CpLP) on blood homeostasis in diabetic mice with disseminated bacterial infection.

Identification and Characterization of Organic Calcium Crystals in the and Latexes.

Latex is usually a milky fluid but can also be clear, brown, or orange. It consists of organic compounds (rubber particles and primary and secondary metabolites) and proteins dispersed in an aqueous phase. Four latex fluids were examined through optical microscopy, and organic crystals were found.

Nicotianin-I: A Tobacco Floral Nectar Peptide with Anticandidal Activity.

Multidrug-resistant microorganisms are major threats to society, leading to the necessity of alternative molecules to fight them back. Antimicrobial peptides (AMPs), especially those derived from plants, have become relevant for multiple reasons. Therefore, this study evaluated six peptides identified in the floral nectar of ornamental tobacco for their effectiveness against four clinically relevant yeast species (Candida albicans, Candida krusei, Candida parapsilosis, and Candida tropicalis).

A novel galectin with triple carbohydrate recognition domain in the parotoid secretion of Rhinella diptycha.

Galectins are a family of animal lectins involved in cell adhesion, tumor differentiation, and apoptosis that can bind reversibly to carbohydrates with a high affinity for β-galactosides. Thus far, however, the primary structure and solved three-dimensional structure have been described for only a few amphibian galectins. Therefore, this work aimed to identify and structurally characterize the galectin (RdG) present in the secretion of the parotid gland of R.

Purification, Characterization, and Antimicrobial Activity Against and of a Highly Stable Type-1 Cystatin from Terminalia catappa L. Seeds.

Introduction: Clinic infections caused by various microorganisms are a public health concern. The rise of new strains resistant to traditional antibiotics has exacerbated the problem. Thus, the search for new antimicrobial molecules remains highly relevant.

Laticifers as in vivo plant cell factories for therapeutic proteins.

The demand for therapeutic proteins is growing annually. Novel approaches for the production of these molecules on a large scale are necessary, especially in plants. Plant laticifers could help provide an in vivo cell system for protein production expression that can reduce costs of production and downstream processing.

Review: Laticifer as a plant defense mechanism.

Laticifers have been utilized as paradigms to enhance comprehension of specific facets of plant ecology and evolution. From the beginning of seedling growth, autonomous laticifer networks are formed throughout the plant structure, extending across all tissues and organs. The vast majority of identified products resulting from laticifer chemistry and metabolism are linked to plant defense.

Class III plant peroxidases: From classification to physiological functions.

Peroxidases (EC 1.11.1.

United we stand, divided we fall: in-depth proteomic evaluation of the synergistic effect of -CBP-PepI and Ciprofloxacin against biofilms.

forms biofilms, a structure that protects bacterial cells, conferring more resistance to difficult treatment. Synthetic peptides surge as an alternative to overcome the biofilm of multidrug-resistant pathogens. -CBP-PepI, when combined with Ciprofloxacin, reduced preformed biofilm by 50% at low concentrations (0.

Proteomic analysis of Cryptostegia grandiflora latex, purification, characterization, and biological activity of two osmotin isoforms.

Although latex fluids are found in >20,000 plant species, the biochemical composition and biological function of their proteins are still poorly explored. Thus, this work aimed to conduct a proteomic analysis of Cryptostegia grandiflora latex (CgLP) for subsequent purification and characterization of an antifungal protein. After 2D-SDS-PAGE and mass spectrometry, 27 proteins were identified in CgLP, including a polygalacturonase inhibitor, cysteine peptidases, pathogenesis-related proteins (PR-4), and osmotins.

Antimicrobial Activity the Essential Oil from Carn. Leaves against Microorganisms of Clinical Interest.

Multiresistant pathogens pose a serious threat to human health. The genus is one class of human pathogenic yeasts responsible for infections affecting healthy and immunocompromised patients. In this context, plant essential oils emerged as a future natural alternative to control the diseases caused by these pathogens.

DVL, lectin from Dioclea violacea seeds, has multiples mechanisms of action against Candida spp via carbohydrate recognition domain.

Lectins are proteins of non-immunological origin with the ability to bind to carbohydrates reversibly. They emerge as an alternative to conventional antifungals, given the ability to interact with carbohydrates in the fungal cell wall inhibiting fungal growth. The lectin from D.

No Chance to Survive: -CBP-PepII Synthetic Peptide Acts on by Multiple Mechanisms of Action.

Multidrug-resistant is an encapsulated yeast causing a high mortality rate in immunocompromised patients. Recently, the synthetic peptide -CBP-PepII emerged as a potent anticryptococcal molecule with an MIC at low concentration. Here, the mechanisms of action of -CBP-PepII were deeply analyzed to provide new information about how it led cells to death.

Giving a Hand: Synthetic Peptides Boost the Antifungal Activity of Itraconazole against .

is a multidrug-resistant pathogen responsible for infections in immunocompromised patients. Here, itraconazole (ITR), a commercial antifungal drug with low effectiveness against , was combined with different synthetic antimicrobial peptides (SAMPs), Mo-CBP3-PepII, RcAlb-PepII, RcAlb-PepIII, PepGAT, and PepKAA. The Mo-CBP3-PepII was designed based on the sequence of MoCBP3, purified from seeds.

New Insights into the Mechanism of Antibacterial Action of Synthetic Peptide -CBP-PepI against .

is a multidrug-resistant opportunistic human pathogen related to various infections. As such, synthetic peptides have emerged as potential alternative molecules. -CBP-PepI has presented great activity against by presenting an MIC at a very low concentration (31.

Essential Oil from Leaves: Anticandidal Potential and Mechanisms of Action.

Antimicrobial drugs are becoming ineffective given the resistance acquired by microorganisms. As such, it is imperative to seek new antimicrobial molecules that could provide a basis for the development of new drugs. Therefore, this work aimed to evaluate the antimicrobial potential and the mechanisms of action of the essential oil extracted from leaves of (named EO) on different fungi and bacteria of clinical importance in both planktonic and biofilm lifestyles.

Use of Calotropis procera cysteine peptidases (CpCPs) immobilized on glyoxyl-agarose for cheesemaking.

Calotropis procera cysteine peptidases (CpCPs) have presented several potential biotechnological applications. Here, these enzymes were immobilized on glyoxyl-agarose (glyoxyl-CpCPs) with yields of 90-95 % and the recovered activities ranged from 10 % to 15 %, according to enzyme loadings (5, 10, 20, 40, and 50 mgBSAeq/g). Spectrophotometric assays and SDS-PAGE showed that the casein hydrolysis by glyoxyl-CpCPs was similar to soluble CpCPs.

Synergistic Antibiofilm Activity between Synthetic Peptides and Ciprofloxacin against .

is a human pathogen known to be resistant to antibiotics since the mid-20th century and is constantly associated with hospital-acquired infections. forms biofilms, which are complex surface-attached communities of bacteria held together by a self-produced polymer matrix consisting of proteins, extracellular DNA, and polysaccharides. Biofilms are resistance structures responsible for increasing bacterial resistance to drugs by 1000 times more than the planktonic lifestyle.

Systematic review of antiprotozoal potential of antimicrobial peptides.

Protozoa is a group of microorganisms that cause neglected tropical diseases, such as malaria, Chagas disease, and Leishmaniasis. Due to the growing demand for new therapeutic agents, antimicrobial peptides (AMPs) have gained attention for antiprotozoal action. A systematic literature review described the current scenario of plant and animal AMPs with action antiprotozoal.

Antifungal Potential of Synthetic Peptides against : Mechanism of Action Studies Reveal Synthetic Peptides Induce Membrane-Pore Formation, DNA Degradation, and Apoptosis.

is a human-pathogenic yeast responsible for pneumonia and meningitis, mainly in patients immunocompromised. Infections caused by are a global health concern. Synthetic antimicrobial peptides (SAMPs) have emerged as alternative molecules to cope with fungal infections, including .

Ornamental tobacco floral nectar is a rich source of antimicrobial peptides.

Although floral nectar is a rich source of nutrients, it is rarely infected by microorganisms. Defense molecules such as proteins have been identified in this fluid, but defense peptides have been largely overlooked. Thus, the aim of this study was to perform an extensive peptidomic analysis of the ornamental tobacco floral nectar to seek peptides involved in nectar defense.

Protective Effect of Latex Proteins on Intestinal Mucositis Induced by 5-Fluorouracil.

Intestinal mucositis is characterized by inflammation and ulceration of the mucosa that affects the gastrointestinal tract and is associated with administering some drugs, such as 5- Fluorouracil (5-FU), conventional chemotherapy used in clinics for cancer therapy. Inside intestinal mucosa, the 5-FU acts, leading to oxidative stress, stimulating the production/release of proinflammatory cytokines, local accumulation of neutrophils and consequent tissue damage. These alterations favor bacterial proliferation, triggering secondary infections, and are responsible for undesired effects such as myelosuppression and diarrhea.

Luffa operculata seed proteins: Identification by LC-ESI-MS/MS and biotechnological potential against Candida albicans and C. krusei.

L: operculata is a plant commonly found in the North and Northeast of Brazil. Although the regional population knows its medicinal potential, there are few scientific studies about its antimicrobial potential. Thus, this study aimed to characterize the proteins from L.

Plant antimicrobial peptides: An overview about classification, toxicity and clinical applications.

Antimicrobial peptides, also known as AMPs, are cationic and amphipathic molecules found in all living organisms, composing part of the defense mechanisms against various pathogens, including fungi, viruses, bacteria, and nematodes. AMPs derived from plants are the focus of this review because they have gained attention as alternative molecules to overcome pathogen resistance as well as new drugs to combat cancer. Plant AMPs are generally classified based on their sequences and structures, as thionins, defensins, hevein-like peptides, knottins, stable-like peptides, lipid transfer proteins, snakins, and cyclotides.