USP22 promotes neuroblastoma growth, metastasis, and glycolysis via mediating the deubiquitination of PDK1.

Deubiquitinating enzymes are important regulators of cancer progression. We explored the role and regulatory mechanisms of the deubiquitinating enzyme ubiquitin-specific protease 22 (USP22) in neuroblastoma (NB). USP22 expression was upregulated in NB patient tissue samples and its expression correlated with their overall survival.

Targeted elimination of Vancomycin resistance gene vanA by CRISPR-Cas9 system.

Objective: The purpose of this study is to reduce the spread of the vanA gene by curing the vanA-harboring plasmid of vancomycin-resistant using the CRISPR-Cas9 system.

Methods: Two specific spacer sequence (sgRNAs) specific was designed to target the vanA gene and cloned into plasmid CRISPR-Cas9. The role of the CRISPR-Cas system in the plasmid elimination of drug-resistance genes was verified by chemically transformation and conjugation delivery methods.

Effects of Tearing Conditions on the Crack Propagation in a Monolayer Graphene Sheet.

The path of crack propagation in a graphene sheet is significant for graphene patterning via the tearing approach. In this study, we evaluate the fracture properties of pre-cracked graphene during the tearing process, with consideration of the effects of the aspect ratio, loading speed, loading direction, and ambient temperatures on the crack propagation in the monolayer sheet. Some remarkable conclusions are drawn based on the molecular dynamic simulation results, i.

Bonding few-layered graphene via collision with high-speed fullerenes.

Graphene, as a typical two-dimensional material, is popular in the design of nanodevices. The interlayer relative sliding of graphene sheets can significantly affect the effective bending stiffness of the few-layered graphene. For restricting the relative sliding, we adopted the atomic shot peening method to bond the graphene sheets together by ballistic C60 fullerenes from its two surfaces.

Analogous Diamondene Nanotube Structure Prediction Based on Molecular Dynamics and First-Principle Calculations.

A concentric twin tube (CTT) can be built by placing a carbon nanotube (CNT) in another identical CNT. Different from diamondene nanotubes, a stable CTT has no inter-shell covalent bond. As a prestressed double-walled nanotube, CTT has a lower structural stability at a finite temperature.

Recoverability of a gigahertz rotation-translation nanoconvertor with hydrogenated deformable rotor at room temperature.

To design a rotor with recoverable deformation for conversion between rotation and translation in a nanodevice, an internally hydrogenated deformable part (HDP) was introduced in the carbon nanotube-based rotor. Initially, under van der Waals (vdW) force, the hydrogenated areas on the HDP curved toward the rotating axis. When a rotational frequency was exerted on the rotor, the hydrogenated parts on the HDP were separated under strong centrifugal force.

[Effect of acupoint massage dominant early comprehensive intervention on the prognosis of premature infants with brain injury].

Objective: To explore the effect of acupoint massage dominant early comprehensive intervention on the prognosis of premature infants with brain injury.

Methods: Totally 210 premature infants with brain injury were assigned to the intervention group (112 cases) and the control group (98 cases). All patients received routine therapy (medicinal + routine care instructions).