Bimetallic Plasmonic Nanozyme-Based Microneedle for Synergistic Ferroptosis Therapy of Melanoma.

Melanoma is the most common malignant skin tumor, characterized by complexity, invasiveness, and heterogeneity. Conventional therapies often yield poor outcomes, posing significant clinical challenges. Here, a microneedle (MN) patch that integrates nanozyme and traditional Chinese medicine (TCM) for ferroptosis pathway-dependent combined therapy of melanoma is designed.

A highly sensitive and fast-response fluorescence nanoprobe for in vivo imaging of hypochlorous acid.

Fluorescent probes for in vivo hypochlorous acid (HClO) imaging often face challenges of low selectivity and high cytotoxicity, largely due to poor analyte recognition and water-insoluble aromatic skeletons. To address this, we synthesized fluorescein hydrazide by introducing a spiro-lactam unit into fluorescein, which offers high emission intensity and molar absorption. The five-membered heterocycle in fluorescein hydrazide is selectively disrupted by HClO, enhancing the conjugated system and electron delocalization of the fluorophore, resulting in highly sensitive fluorescence detection of HClO.

SERS Platform for Integrated Enrichment, Isolation, and Identification of Multiple Respiratory Viruses in a Single Assay Using 3D Stereoscopic SERS Tags and Flocked Swabs.

Influenza (flu) and severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) exhibit similar clinical symptoms, complicating the diagnosis and clinical management of these critical respiratory infections. Thus, there is an urgent need for rapid on-site detection technologies that can simultaneously detect SARS-CoV-2 and influenza A viruses. Here, we have developed the first platform that combines in situ sampling with immune swabs and multichannel surface-enhanced Raman spectroscopy (SERS) for simultaneous screening of these two respiratory viruses in a single assay.

A highly sensitive point-of-care detection platform for Salmonella typhimurium by integrating magnetic enrichment and fluorescent CsPbBr@SiO.

A platform was designed based on FeO and CsPbBr@SiO for integrated magnetic enrichment-fluorescence detection of Salmonella typhimurium, which significantly simplifies the detection process and enhances the working efficiency. FeO served as a magnetic enrichment unit for the capture of S. typhimurium.

PEI-Mediated Assembly of FeO onto SiO-Encapsulated CsPbBr for Highly Sensitive Fluorescent Lateral Flow Immunoassay.

The conventional lateral flow immunoassay (LFIA) method using colloidal gold nanoparticles (Au NPs) as labeling agents faces two inherent limitations, including restricted sensitivity and poor quantitative capability, which impede early viral infection detection. Herein, we designed and synthesized CsPbBr perovskite quantum dot-based composite nanoparticles, CsPbBr@SiO@FeO (CSF), which integrated fluorescence detection and magnetic enrichment properties into LFIA technology and achieved rapid, sensitive, and convenient quantitative detection of the SARS-CoV-2 virus N protein. In this study, CsPbBr served as a high-quantum-yield fluorescent signaling probe, while SiO significantly enhanced the stability and biomodifiability of CsPbBr.

In Situ Growth of Conductive Metal-Organic Framework onto CuO for Highly Selective and Humidity-Independent Hydrogen Sulfide Detection in Food Quality Assessment.

The sensitivity of chemiresistive gas sensors based on metal oxide semiconductors (MOSs) has been inherently affected by ambient humidity because their reactive oxygen species are easily hydroxylated by water molecules, which significantly reduces the accuracy of the gas sensors in food quality assessment. Although conventional metal organic frameworks (MOFs) can serve as coatings for MOSs for humidity-independent gas detection, they have to operate at high working temperatures due to their low or nonconductivity, resulting in high power consumption, significant manufacturing inconvenience, and short-term stability due to the oxidation of MOFs. Here, the conductive and thickness-controlled CuHHTP (HHTP = 2,3,6,7,10,11-hexahydroxytriphenylene)-coated CuO are developed by combining in situ etching and layer-by-layer liquid-phase growth method, which achieves humidity-independent detection of HS at room temperature.

Multifunctional Nanoprobe-Amplified Enzyme-Linked Immunosorbent Assay on Capillary: A Universal Platform for Simple, Rapid, and Ultrasensitive Dual-Mode Pathogen Detection.

Although the traditional enzyme-linked immunosorbent assay (ELISA) has been widely applied in pathogen detection and clinical diagnostics, it always suffers from complex procedures, a long incubation time, unsatisfying sensitivity, and a single signal readout. Here, we developed a simple, rapid, and ultrasensitive platform for dual-mode pathogen detection based on a multifunctional nanoprobe integrated with a capillary ELISA (CLISA) platform. The novel capture antibodies-modified capillaries can act as a swab to combine in situ trace sampling and detection procedures, eliminating the dissociation between sampling and detection in traditional ELISA assays.

A simple sensor based on 1,8-naphthalimide with large Stokes shift for detection of hypochlorous acid in living cells.

Hypochlorous acid (HOCl), one of the most reactive and deleterious reactive oxygen species (ROS), plays a vital role in many pathological and physiological processes. However, as a result of the highly reactive and diffusible nature of HOCl, its uncontrolled production may lead to an adverse effect on host physiology. Because of its biological importance, many efforts have been focused on developing selective fluorescent probes to image HOCl.

A "weak acid and weak base" type fluorescent probe for sensing pH: mechanism and application in living cells.

A simple pH fluorescent probe, -(6-morpholino-1, 3-dioxo-1-benzo[]isoquinolin-2(3)-yl) isonicotinamide (NDI), based on naphthalimide as the fluorophore and isonicotinic acid hydrazide as the reaction site was synthesized and characterized. It is useful for monitoring acidic and alkaline pH. The results of pH titration indicated that NDI exhibits obvious emission enhancement with a p of 4.

Research progress in the development of organic small molecule fluorescent probes for detecting HO.

Hydrogen peroxide (HO), as an important signaling molecule during biological metabolism, is a key member of the reactive oxygen species (ROS) family. The excess of HO will lead to oxidative stress, which is a crucial factor in the production of various ROS-related diseases. In order to study the diverse biological roles of HO in cells and animal tissues, many methods have been developed to detect HO.