Rapid MS-Based Single-Cell Analysis Assisted by Photochemical Derivatization in Living Cell Membranes Reveals Lipidomic Patterns with Located C═C Bonds.

Traditional lipidomics methods are not well-suited for studying unsaturated lipids containing located C═C bonds in single cells. Here, we proposed a single-cell mass spectrometry (MS) platform assisted by a photochemical derivatization (PCD) method termed isible-light-activated u(bpy)-catalyzed inglet xygen-mediated ydroperoxidation (VRSOH), for the rapid and simultaneous detection of multiple lipid C═C location isomers in living cell membranes at the single-cell level. First, the VRSOH method combined with MS analysis achieved the localization of C═C bonds in mono- and polyunsaturated lipids and cellular lipid extracts.