Ketogenic Diet Modulates Gut Microbiota-Brain Metabolite Axis in a Sex- and Genotype-Specific Manner in APOE4 Mice.

The apolipoprotein E4 (APOE4) allele is the strongest genetic risk factor for late-onset Alzheimer's disease (AD), associated with early brain metabolic dysfunction and gut microbiome alterations. Targeting these early changes through dietary interventions may reduce AD risk in asymptomatic carriers. This study evaluated whether a ketogenic diet (KD) could reshape the gut microbiome and enhance key brain metabolite levels in young APOE4 mice, using APOE3 mice as a neutral-risk comparison.

Rapamycin enhances neurovascular, peripheral metabolic, and immune function in cognitively normal, middle-aged APOE4 Carriers: genotype-dependent effects compared to non-carriers.

Rapamycin, known for its anti-aging properties, shows promise as a preventive strategy for Alzheimer's disease (AD) in APOE4 carriers-the highest-risk group for late-onset AD. Here we show that a 4-week open-label trial of low-dose Rapamycin (Sirolimus; 1mg/day) significantly improved cerebral blood flow (CBF) relative to baseline in cognitively normal APOE4 carriers (E4(+)) aged 45-65. It also reduced inflammatory cytokines, enhanced lipid metabolism, increased short-chain fatty acids (SCFA) and enriched gut microbiome composition linked to SCFA production.

mTOR inhibition enhances synaptic and mitochondrial function in Alzheimer's disease in an APOE genotype-dependent manner.

Apolipoprotein ε4 (APOE4) carriers develop brain metabolic dysfunctions decades before the onset of Alzheimer's disease (AD). A goal of the study is to identify if rapamycin, an inhibitor for the mammalian target of rapamycin (mTOR) inhibitor, would enhance synaptic and mitochondrial function in asymptomatic mice with human APOE4 gene (E4FAD) before they showed metabolic deficits. A second goal is to determine whether there may be genetic-dependent responses to rapamycin when compared to mice with human APOE3 alleles (E3FAD), a neutral AD genetic risk factor.

: unprecedented L-dopa content and its role in neurodegenerative and inflammatory conditions.

Unlabelled: Genus encompasses several plant species renowned for their utilization in traditional Ayurvedic medicine for the treatment of Parkinson's disease, chiefly due to their exceptionally high L-dopa content relative to other plants. However, limited information exists regarding , a newly identified species within the genus. This study unveils a remarkable L-dopa content of 174.

Gut microbiome association with brain imaging markers, APOE genotype, calcium and vegetable intakes, and obesity in healthy aging adults.

Introduction: Advanced age is a significant factor in changes to brain physiology and cognitive functions. Recent research has highlighted the critical role of the gut microbiome in modulating brain functions during aging, which can be influenced by various factors such as apolipoprotein E (APOE) genetic variance, body mass index (BMI), diabetes, and dietary intake. However, the associations between the gut microbiome and these factors, as well as brain structural, vascular, and metabolic imaging markers, have not been well explored.

Prebiotic inulin enhances gut microbial metabolism and anti-inflammation in apolipoprotein E4 mice with sex-specific implications.

Gut dysbiosis has been identified as a crucial factor of Alzheimer's disease (AD) development for apolipoprotein E4 (APOE4) carriers. Inulin has shown the potential to mitigate dysbiosis. However, it remains unclear whether the dietary response varies depending on sex.

Microbial degradation of poultry feather biomass in a constructed bioreactor and application of hydrolysate as bioenhancer to vegetable crops.

Bioconversion of recalcitrant keratinous biomass is one of the greatest ways to utilize products of feather hydrolysis and recycle them into bionetwork. Present study revealed 87% degradation of poultry feathers within 48 h in a constructed bioreactor using Chryseobacterium sp. RBT.

RP-HPLC analysis of diterpene lactones in leaves and stem of different species of .

In the present study diterpene lactones were quantified in leaves and stem of different species of collected from Western Ghats of India using reverse phase high performance liquid chromatography (RP-HPLC) method. Different populations of AA (), AE (), ALn ( var. ), ALw ( var.

Processing Effect on L-DOPA, In Vitro Protein and Starch Digestibility, Proximate Composition, and Biological Activities of Promising Legume: .

: In the present investigation, the effect of different cooking processes on L-DOPA level, phenolics contents, in vitro protein (IVPD) and starch digestibility (IVSD), and proximate composition with in vitro anti-inflammatory and antioxidant potential of (MM) has been evaluated. : The L-DOPA and major phenolics acids quantification of processed samples were done by a reverse-phase high-performance liquid chromatography (RP-HPLC) technique. Proximate composition, elemental quantification, and in vitro protein and starch digestibility of the samples were carried out by using spectrophotometric analysis.

Ultrasound-Assisted Aqueous Extraction of Phenolic, Flavonoid Compounds and Antioxidant Activity of Mucuna macrocarpa Beans: Response Surface Methodology Optimization.

Objective: This investigation was undertaken to optimize the effective extraction of total phenolics content (TPC), total flavonoids content (TFC), and antioxidant activity from the Mucuna macrocarpa (MM) beans. An ultrasound-assisted extraction (UAE) technique with water as an effective solvent was proposed for the response surface methodology (RSM) optimization.

Methods: A three-level, two-factor central composite design (CCD) was employed to reveal the optimal points of variables.

Response surface methodology optimization for sorption of malachite green dye on sugarcane bagasse biochar and evaluating the residual dye for phyto and cytogenotoxicity.

In the present study, sorption and detoxification of malachite green (MG) dye was executed using biochar resulting after pyrolysis of agro-industrial waste at 400, 600 and 800 °C. Maximum sorption of MG dye (3000 mg/L) was observed on the sugarcane bagasse biochar (SCB) prepared at 800 °C. The interactive effects of different factors like dye concentration, time, pH and temperature on sorption of MG dye were investigated using response surface methodology (RSM).