Spatiotemporally Resolved Profiling of Protein Movement by TransitID.

Protein functions are tightly associated with their locations and can be dynamically regulated by movement between cellular regions. Traditional methods, such as fluorescent protein-based imaging and fractionation-coupled proteomics, have fundamental limitations in the unbiased discovery of protein trafficking events. Proximity labeling (PL) technology has emerged as a versatile tool in spatial proteomics based on genetically encoded promiscuous enzymes, which generate reactive species to tag endogenous proteins.

Multi-scale conv-attention U-Net for medical image segmentation.

U-Net-based network structures are widely used in medical image segmentation. However, effectively capturing multi-scale features and spatial context information of complex organizational structures remains a challenge. To address this, we propose a novel network structure based on the U-Net backbone.

Tibetan tea theabrownin Ameliorates Ethanol-Induced defects and modulates regulatory pathways in zebrafish.

This study investigates the therapeutic potential of theabrownin, a polymer from Tibetan tea, in reducing ethanol-induced developmental abnormalities using a zebrafish model. Zebrafish embryos were pretreated with varying concentrations of theabrownin before ethanol exposure, followed by an assessment of their developmental morphology, oxidative stress markers, and metabolic pathways. The 100 µg/mL dose yielded the most significant reduction in malformations, while the 50 µg/mL and 200 µg/mL doses also demonstrated moderate improvements.

Insights into the low-temperature rapid catalytic pyrolysis and remediation mechanism of weathered petroleum-contaminated saline-alkali soil using Beta zeolite.

Pyrolysis technique is considered to have great potential in the remediation of petroleum-contaminated soil, but it still has difficulties such as high energy consumption for the degradation of complex petroleum hydrocarbons and the deterioration of soil quality after treatment. In this study, the low-temperature rapid catalytic pyrolysis was realized using Beta zeolite to assist in remediating weathered petroleum-contaminated saline-alkali soil. Under the action of Beta zeolite, the removal efficiency of petroleum hydrocarbons reached 81% after pyrolysis treatment for 10 min at 250 °C, which was reduced to regulatory standard.

Using an integrated feature-based molecular network and lipidomics approach to reveal the differential lipids in yak shanks and flanks.

Yak shanks and flanks are often used as food ingredients, but the lipid composition of these two parts may differ significantly. These meat parts were subjected to a lipidomics analysis using UHPLC-Q-Obitrap. Several computational tools, including feature-based molecular networks, ms-dial, and lipidone, were used to perform deep mining on the entire dataset.