MicroRNA dynamics and networks to liver fibrosis-related genes during hepatic stellate cell activation by agalactosyl IgG and transforming growth factor-β1.

The pathogenic effects of agalactosyl IgG on activating hepatic stellate cells (HSCs) and promoting liver fibrogenesis have been previously reported. However, the dynamics of miRNAome and their association with fibrosis-related genes during HSC activation by agalactosyl IgG remain unclear. We performed small RNA sequencing in human LX-2 HSCs at different time points after agalactosyl IgG treatment.

SUMO-LMNet: Lossless mapping network for predicting SUMOylation sites in SUMO1 and SUMO2 using high-dimensional features.

Accurate SUMOylation site prediction is crucial for deciphering gene regulation and disease mechanisms. However, distinguishing SUMO1 and SUMO2 modifications remains a major challenge due to their structural similarities. Conventional prediction models often struggle to differentiate between these paralogues, limiting their applicability in biological research.

Arabidopsis research in 2030: Translating the computable plant.

Plants are essential for human survival. Over the past three decades, work with the reference plant Arabidopsis thaliana has significantly advanced plant biology research. One key event was the sequencing of its genome 25 years ago, which fostered many subsequent research technologies and datasets.

Nurse's perspective: Evaluating importance and satisfaction with hospital patient infotainment terminal.

Background: The Patient Infotainment Terminal (PIT) plays a pivotal role in Smart Health, enabling hospitals to actively pursue the objective of fostering Shared Decision-Making. By providing General information, Medical information, and Entertainment options, the system fosters effective patient-clinician communication and significantly elevates the standard of care.

Objective: This study aimed to investigate how registered nurses utilized the PIT and prioritized functions based on their perception of importance and satisfaction to find out high-importance but low-satisfaction PIT functions.

Rice Husk Silica Liquid Enhances Autophagy and Reduces Overactive Immune Responses via TLR-7 Signaling in Lupus-Prone Models.

Systemic lupus erythematosus (SLE) is a chronic autoimmune disorder characterized by widespread inflammation and multi-organ damage. Toll-like receptor 7 (TLR-7) and autophagy have been implicated in SLE pathogenesis. Rice husk silica liquid (RHSL) has shown potential for modulating inflammatory responses, but its effects on SLE have not been thoroughly investigated.

Substitutions in the nonactive site of the passenger domain on the activity of immunoglobulin A1 protease.

Immunoglobulin A1 (IgA1) protease is a critical virulence factor of that facilitates bacterial mucosal infection. This study investigates the effect of gene polymorphism on the enzymatic activity of IgA1 protease. The IgA1 protease activity was examined in the Rd KW20 strain and 51 isolates.

Agalactosyl IgG induces liver fibrogenesis via Fc gamma receptor 3a on human hepatic stellate cells.

The relevance of aberrant serum IgG N-glycosylation in liver fibrosis has been identified; however, its causal effect remains unclear. Because hepatic stellate cells (HSCs) contribute substantially to liver fibrosis, we investigated whether and through which mechanisms IgG N-glycosylation affects the fibrogenic properties of HSCs. Analysis of serum IgG N-glycome from 151 patients with chronic hepatitis B or liver cirrhosis revealed a positive correlation between Ishak fibrosis grading and IgG with agalactosyl N-glycoforms on the crystallizable fragment (Fc).

Amino acid substitutions in the region between RpoB clusters II and III on rifampin susceptibility in Haemophilus influenzae.

Background: Rifampin is a potent chemoprophylactic antibiotic for Haemophilus influenzae infection, and the resistance rate in H. influenzae is low. In this study, we assessed rifampin resistance-related genetic variations in H.

CIPK15-mediated inhibition of NH transport protects from submergence.

Ammonium (NH) serves as a vital nitrogen source for plants, but it can turn toxic when it accumulates in excessive amounts. Toxicity is aggravated under hypoxic/anaerobic conditions, e.g.

Fluorescent Biosensor Imaging of Nitrate in .

Nitrate (NO) is an essential element and nutrient for plants and animals. Despite extensive studies on the regulation of nitrate uptake and downstream responses in various cells, our knowledge of the distribution of nitrogen forms in different root cell types and their cellular compartments is still limited. Previous physiological models have relied on in vitro biochemistry and metabolite level analysis, which limits the ability to differentiate between cell types and compartments.

Molecular characterization of multidrug-resistant non-typeable Haemophilus influenzae with high-level resistance to cefuroxime, levofloxacin, and trimethoprim-sulfamethoxazole.

Background: Non-typeable Haemophilus influenzae (NTHi) has become the major cause of invasive H. influenzae diseases in the post-H. influenzae type b vaccine era.

Molecular characterization of cefepime and aztreonam nonsusceptibility in Haemophilus influenzae.

Background: Cefepime and aztreonam are highly efficacious against H. influenzae, and resistant strains are rare. In this study, we isolated cefepime- and aztreonam-nonsusceptible H.

A Novel Phytogenic Formulation, EUBIO-BPSG, as a Promising One Health Approach to Replace Antibiotics and Promote Reproduction Performance in Laying Hens.

Gut microbiota play a key role in health maintenance and disease pathogenesis in animals. Dietary phytochemicals are crucial factors shaping gut bacteria. Here, we investigated the function and mechanism of a phytogenic formulation, EUBIO-BPSG (BP), in laying hens.

In vivo visualization of nitrate dynamics using a genetically encoded fluorescent biosensor.

Nitrate (NO) uptake and distribution are critical to plant life. Although the upstream regulation of NO uptake and downstream responses to NO in a variety of cells have been well studied, it is still not possible to directly visualize the spatial and temporal distribution of NO with high resolution at the cellular level. Here, we report a nuclear-localized, genetically encoded fluorescent biosensor, which we named NitraMeter3.

Concept of Fluorescent Transport Activity Biosensor for the Characterization of the Arabidopsis NPF1.3 Activity of Nitrate.

The NRT1/PTR FAMILY (NPF) in Arabidopsis () plays a major role as a nitrate transporter. The first nitrate transporter activity biosensor NiTrac1 converted the dual-affinity nitrate transceptor NPF6.3 into fluorescence activity sensors.

Feedback inhibition of AMT1 NH-transporters mediated by CIPK15 kinase.

Background: Ammonium (NH), a key nitrogen form, becomes toxic when it accumulates to high levels. Ammonium transporters (AMTs) are the key transporters responsible for NH uptake. AMT activity is under allosteric feedback control, mediated by phosphorylation of a threonine in the cytosolic C-terminus (CCT).

Plasma proteome atlas for differentiating tumor stage and post-surgical prognosis of hepatocellular carcinoma and cholangiocarcinoma.

Although mass spectrometry-based plasma proteomics enables sensitive and large-scale discovery and validation of biomarkers for various diseases, its integrative application to hepatocellular carcinoma (HCC) and cholangiocarcinoma (CCA) is not well investigated. Therefore, we analyzed albumin- and immunoglobulin G-depleted plasma samples from 148 and 60 patients with HCC and CCA, respectively, using liquid chromatography-tandem mass spectrometry. The algorithm used to measure the content of each protein was the percentage of exponentially modified protein abundance index.

Extensively drug-resistant Haemophilus influenzae - emergence, epidemiology, risk factors, and regimen.

Background: Concern about Haemophilus influenzae infection has been increasing over recent decades. Given the emergence of H. influenzae with severe drug resistance, we assessed the prevalence of as well as risk factors and potential therapies for extensively drug-resistant (XDR) H.

Telbivudine on IgG-associated hypergammaglobulinemia and TGF-β1 hyperactivity in hepatitis B virus-related liver cirrhosis.

As debate rumbles on about whether anti-hepatitis B virus (HBV) nucleos(t)ide analogue treatments modulate host immune system during end-stage liver diseases, we studied effects of two potent anti-HBV agents, telbivudine or entecavir, on humoral immune activities including cytokine secretion, immunoglobulin production, and IgG-Fc agalactosylation, which is known to induce proinflammatory responses, in liver cirrhosis. Serum IgG-Fc N-glycan structures in patients with HBV-related liver cirrhosis, who had received either telbivudine treatment or entecavir treatment for at least 48 weeks were analyzed using liquid chromatography tandem-mass spectrometry. Levels of cytokines and each immunoglobulin isotype were measured using enzyme-linked immunosorbent assays.

TRANSPORTER OF IBA1 Links Auxin and Cytokinin to Influence Root Architecture.

Developmental processes that control root system architecture are critical for soil exploration by plants, allowing for uptake of water and nutrients. Conversion of the auxin precursor indole-3-butyric acid (IBA) to active auxin (indole-3-acetic acid; IAA) modulates lateral root formation. However, mechanisms governing IBA-to-IAA conversion have yet to be elucidated.

Theoretical integration of user satisfaction and technology acceptance of the nursing process information system.

Background: The nursing process system (NPS) is used to establish the nursing process involving assessment, diagnosis, planning, intervention, and evaluation in solving the health problems of patients.

Objectives: The factors influencing the use of the NPS by nurses were analyzed based on user satisfaction and technology acceptance within the 3Q (service quality, information quality, and system quality) model.

Methods: In this cross-sectional quantitative study, the valid responses of 222 nurses to a questionnaire were obtained; these nurses worked at eight hospitals affiliated with public organizations in Taiwan.