sp. nov. and sp. nov.; Two Novel Fungal Species Isolated from Soil in Korea.

During an investigation of microfungi from various ecosystems in South Korea, two novel fungal species (KNUF-22-094 and KNUF-23-321C) were discovered within the genera and . The strain KNUF-22-094 stands out among other species due to its distinctive conidiogenous cells and conidial size ranges. The conidiogenous cells of KNUF-22-094 measured 2.

Isolation and Characterization of Four Unreported Species Isolated from the Freshwater Environments in Korea.

The fungal species of the genus can be found across a diverse array of environments. The infrageneric classification of the genus has been studied with comparison of morphological and phylogenetical features, derived into two subgenus, 32 sections, and 89 series. In this study, 11 fungal strains were isolated from freshwater environments, plant litter, and nearby substrates in Korea and were identified as previously unreported species.

Identification of Fusarium Basal Rot Pathogens of Onion and Evaluation of Fungicides against the Pathogens.

Onion ( L.) is an economically important vegetable crop worldwide. However, various fungal diseases, including Fusarium basal rot (FBR), neck rot, and white rot, reduce onion production or bulb storage life.

Pectobacterium jejuense sp. nov. Isolated from Cucumber Stem Tissue.

A single Pectobacterium-like strain named 13-115 was isolated from a specimen of diseased cucumber stem tissue collected on Jeju Island, South Korea. The strain presented a rod-like shape and was negative for Gram staining. When grown on R2A medium at 25 °C, strain 13-115 formed round, convex and white colonies.

Using RNA-Sequencing Data to Examine Tissue-Specific Garlic Microbiomes.

Garlic () is a perennial bulbous plant. Due to its clonal propagation, various diseases threaten the yield and quality of garlic. In this study, we conducted in silico analysis to identify microorganisms, bacteria, fungi, and viruses in six different tissues using garlic RNA-sequencing data.

QTL Mapping for Gummy Stem Blight Resistance in Watermelon ( spp.).

Watermelon () is an economically important fruit crop worldwide. Gummy stem blight (GSB) is one of the most damaging diseases encountered during watermelon cultivation. In the present study, we identified quantitative trait loci (QTLs) associated with GSB resistance in an F population derived from a cross between maternal-susceptible line '920533' () and the paternal-resistant line 'PI 189225' ().

Occurrence of Cercospora Leaf Spot Caused by cf. on Melon in Korea.

In 2016, a cercosporoid fungus was found from leaf spot symptoms on melon in Korea. The fungus isolated from the plant was identified based on morphological characteristics and sequence analyses of five genes (ITS rDNA, translation elongation factor 1-α, actin, calmodulin, and histone H3). The fungal isolate was found to be pathogenic to melon.

Causing Anthracnose on Orchids in Korea.

A species was isolated from leaves of exhibiting symptoms of anthracnose. In this study, the isolates obtained were identified based on recent taxonomic approaches for the genus . The identity of the causal pathogen was confirmed using morphological data and phylogenetic analysis of combined multi-gene dataset (internal transcribed spacer, glyceraldehyde 3-phosphate dehydrogenase, chitin synthase-1, actin, histone3, beta-tubulin, and calmodulin).

Comparative Microbiome Study of Mummified Peach Fruits by Metagenomics and Metatranscriptomics.

The dried peach fruits clinging to peach trees or lying on the ground nearby are known as mummified peach fruits. Here, we examined the microbiome communities of three different mummified peach fruits from the nectarine cultivar "Hahong" by DNA- and RNA-sequencing. We found the dominance of followed by , , and in the mummified peach fruits.

High-Quality Genome Assembly of , the Causal Agent of Onion Downy Mildew.

is an obligate biotrophic oomycete that causes downy mildew on onion (). Onion is an important crop worldwide, but its production is affected by this pathogen. We sequenced the genome of using the PacBio sequencing platform, and de novo assembly resulted in 74 contigs with a total contig size of 29.

Loop-Mediated Isothermal Amplification for the Detection of pv. in Peaches.

To detect pv, a loop-mediated isothermal amplification (LAMP) detection method were developed. The LAMP assay was designed to test crude plant tissue without pre-extraction, or heating incubation, and without advanced analysis equipment. The LAMP primers were designed by targeting an ABC transporter ATP-binding protein, this primer set was tested using the genomic DNA of and non- strains, and a ladder product was generated from the genomic DNA of pv strain but not from 12 other species strains and 6 strains of other genera.

Whole-Genome Sequence of Erwinia persicina B64, Which Causes Pink Soft Rot in Onions.

Erwinia persicina B64 was isolated from rotten onions in cold-storage facilities. Here, we report the complete genome sequence of E. persicina B64, which contains 5,070,450 bp with 55.

Cutaneous Microflora from Geographically Isolated Groups of , an Insect Vector of Diverse Plant Pathogens.

Larvae of , an insect vector that transports plant pathogens, were sampled from geographically isolated regions in Korea to identify their cutaneous fungal and bacterial flora. Sampled areas were chosen within the distribution range of ; each site was more than 91 km apart to ensure geographical segregation. We isolated 76 microbial (fungi and bacteria) strains (site 1, 29; site 2, 29; site 3, 18 strains) that were identified on the basis of morphological differences.

New Recorded Species in Three Genera of the Sordariomycetes in Korea.

In an ongoing survey of Korean indigenous fungi, three fungal strains belonging to the Sordariomycetes were isolated from soil samples. These strains were designated KNU16-001, KNU16-002, and KNU16-009, and identified as , , and , respectively, based on morphological characterization and phylogenetic analysis using internal transcribed spacer region sequences of ribosomal DNA. This is the first report of these species in Korea.

First Report of Leaf Spot Caused by on Black Chokeberry () in Korea.

In July 2015, diseased leaves of black chokeberry () were observed in Danyang and Gochang, Korea. The symptoms appeared as circular or irregular brown leaf spots, from which was isolated. The isolates were cultured on potato dextrose agar, and their morphological characteristics were observed under a light microscope.

First Report of Fusarium subglutinans Causing Leaf Spot Disease on Cymbidium Orchids in Korea.

In 2006~2010, leaf spot symptoms, that is, small, yellow spots that turned into dark brown-to-black lesions surrounded by a yellow halo, were observed on Cymbidium spp. in Gongju, Taean, and Gapyeong in Korea. A Fusarium species was continuously isolated from symptomatic leaves; in pathogenicity testing, isolates caused leaf spot symptoms consisting of sunken, dark brown lesions similar to the original ones.

Development of a Species-specific PCR Assay for Three Xanthomonas Species, Causing Bulb and Flower Diseases, Based on Their Genome Sequences.

In this study, we developed a species-specific PCR assay for rapid and accurate detection of three Xanthomonas species, X. axonopodis pv. poinsettiicola (XAP), X.

Complete genome sequence of Spirosoma radiotolerans, a gamma-radiation-resistant bacterium isolated from rice field in South Korea.

Spirosoma radiotolerans is a Gram-negative, short rod-shaped and gamma-radiation-resistant bacterium isolated from rice field in South Korea (GPS; 37°34'30″N, 127°00'30″E). The complete genome of S. radiotolerans consists of a chromosome (7,029,352bp).

Survey of Cherry necrotic rusty mottle virus and Cherry green ring mottle virus incidence in Korea by Duplex RT-PCR.

The incidence of Cherry necrotic rusty mottle virus (CNRMV) and Cherry green ring mottle virus (CGRMV) have recently been occurred in Korea, posing a problem for sweet cherry cultivation. Since infected trees have symptomless leaves or ring-like spots on the pericarp, it is difficult to identify a viral infection. In this study, the incidence of CNRMV and CGRMV in sweet cherry in Gyeongbuk province was surveyed using a newly developed duplex reverse transcriptase polymerase chain reaction (RT-PCR) method that can detect both viruses in a single reaction.

Draft Genome Sequence of Chryseobacterium sp. Strain P1-3, a Keratinolytic Bacterium Isolated from Poultry Waste.

Chryseobacterium sp. strain P1-3, harboring keratin degrading activity, has recently been isolated from poultry waste. Here, we report the 4.

Outbreak of Rust Caused by Coleosporium asterum on Solidago virgaurea var. gigantea in Ulleung-do.

From June to October 2012, severe outbreaks of rust disease on Solidago virgaurea var. gigantea were observed in 5 different regions of Ulleung-do, Korea. Typical rust symptoms appeared as yellow spots, uredinia on leaves, and massive urediniospores covering the entire plant.

Characterization of Species of Cladobotryum which Cause Cobweb Disease in Edible Mushrooms Grown in Korea.

Four Cladobotryum isolates were collected from four different commercially grown mushroom types infected with cobweb disease in Cheongdo-gun and Chilgok-gun of Gyeongbuk Province, Korea in 2010. The isolates were identified as C. mycophilum from Agaricus bisporus and Pleurotus eryngii, C.

Biological Characterization of Marssonina coronaria Associated with Apple Blotch Disease.

Marssonina coronaria associated with apple blotch disease causes severe premature defoliation, and is widely distributed in Korea. Thirteen isolates were collected from orchards located in Gyeongbuk Province from 2005~2007. All isolates displayed over 99.