Longitudinally extensive transverse myelitis as a manifestation of neuro-chikungunya in a lupus patient: a case report.

Background: Myeloneuropathy is rare complication of Chikungunya virus (CHIKV) infection which may have an underlying immune-mediated pathogenesis.

Case Presentation: We describe a 31-year old female patient with systemic lupus erythematosus (SLE) presenting with acute/hyperacute bulbar and medullary fever syndrome with ascending tetraparesis associated with arthritis, requiring differential diagnosis. The analysis of the cerebrospinal fluid (CSF) revealed inflammatory markers, high protein levels, high lymphocyte and neutrophil counts, and reduced glucose.

Analytical Geometry Description, Clinical Node Distribution, and Geometric Performance of ZAP-X and Its Comparison With Gamma Knife.

In radiotherapy, it is increasingly common to find specialized devices in different treatment techniques. The ZAP-X is a radiosurgery system which features a sophisticated and complex geometric design that allows to deliver a high dose of precisely targeted radiation while minimizing the radiation effect on the surrounding normal brain tissue. Its combination of yoked gimbals allows a maximum theoretical geometric efficiency of [Formula: see text] steradian solid angle coverage, which means that it is possible to cover 71% of the surface around the patient's head, making the ZAP-X the commercially available radiosurgery system with the largest beam coverage.

Relationship between psychosocial risk factors at work and musculoskeletal symptoms in university professors.

Introduction: Musculoskeletal disorders are prevalent among university professors. With the expansion of private higher education and the increasing demands on academic staff, psychosocial risk factors may exacerbate these conditions beyond ergonomic challenges.

Objectives: To investigate the relationship between psychosocial risk factors and musculoskeletal symptoms among university professors in the private sector.

Unveiling Novel Arginase Inhibitors for Cutaneous Leishmaniasis Using Drug Repurposing and Virtual Screening Approaches.

Leishmaniasis is a neglected tropical disease with a significant global health burden, particularly in developing countries, where it accounts for approximately 1.6 million new infections annually. Current therapeutic options are limited by severe adverse effects, toxicity, and drug resistance, highlighting the urgent need for novel treatment strategies.

State of the Art in Novel Treatment Strategies in Rheumatoid Arthritis: A Brief Review.

Rheumatoid arthritis (RA) is a chronic autoimmune inflammatory disease with substantial morbidity and socioeconomic burden. Early diagnosis and treatment are crucial to mitigate disease progression and preserve joint function. Current treatment strategies encompass non-pharmacological and pharmacological approaches, with disease-modifying antirheumatic drugs (DMARDs) being the cornerstone of pharmacotherapy.

Systematic review of the use and challenges of electronic health records in physiotherapy practice.

Objective: To synthesise current evidence on physiotherapists' use of electronic health records (EHRs), with a focus on the determinants of adoption, implementation processes and associated implementation outcomes.

Design: A systematic review employing a narrative synthesis approach.

Data Sources: PubMed, Cochrane, Scopus and Web of Science, covering all records from the inception of each database to 10 May 2024.

Statine-based peptidomimetics as SARS-CoV-2 Papain-like protease inhibitors: in Silico and in vitro studies.

We investigated statin-based peptidomimetic compounds as inhibitors of SARS-CoV-2 papain-like protease (PLpro) by in silico methods, including molecular docking/dynamic simulations and ADMET prediction, as well as enzymatic in vitro assays. Five compounds (LQMed 426, 428, 430, 431, and 432) were identified as having promising interactions with the active site and an allosteric site of PLpro. The docking poses in the active site revealed that the compounds interacted with the catalytic triad (Cys111, His272, and Asp286).

Evaluation of rheumatologists' knowledge of biosimilars.

Introduction: Biosimilars reduce the cost of biologic therapy without compromising safety and effectiveness. In this study we evaluated Brazilian rheumatologists' knowledge and perceptions of biosimilars.

Methods: Cross-sectional and descriptive study based on a questionnaire containing 17 items on familiarity, knowledge and perceptions of biosimilars.

Prevalence of metabolic syndrome in low-income childhood-onset systemic lupus erythematosus patients.

Objective: To determine the prevalence of metabolic syndrome (MetS) in patients with childhood onset Systemic Lupus Erithematosus (cSLE) and controls from Northeastern Brazil and to verify its association with specific SLE parameters and cardiovascular risk factors.

Methods: The prevalence of MetS was assessed cross-sectionally in 58 patients with cSLE and 33 age -matched controls. Information was collected by clinical examination and standardized questionnaires, investigating personal and family history of cardiovascular disease and obesity and socioeconomic and demographic characteristics.

In Vitro and In Silico Assessments of Curcuminoids and Turmerones from as Novel Inhibitors of Arginase.

: The anti- potential of and its derivatives, such as curcuminoids, is well-established, yet their mechanisms of action remain underexplored. This study investigates the inhibitory effects of extracts and curcumin on arginase, a key enzyme in polyamine and trypanothione biosynthesis, and evaluates their antiparasitic activity. : Extracts were prepared via rhizome successive maceration with hexane (HEXCURC), dichloromethane (DCCURC), and ethanol (ETOHCURC) and chemically characterized by a combination of chromatographic and spectrometric methods.

ProBNP, cytokines, and the Nrf2/HO-1 signaling pathway: A cross-sectional study on cardiovascular risk in rheumatoid arthritis.

We evaluated associations between clinical/laboratory findings and serum cytokines, Nrf2/HO-1 pathway expression and cardiovascular risk in both RA patients and controls. Sixty RA patients and 60 controls were included in the study. Serum cytokine and proBNP levels were assessed by ELISA, while serum Nrf2 and HO-1 mRNA levels were quantified by qRT-PCR.

Molecular Modeling and Evaluation of Thioureas and Arylthioureas as Urease Inhibitors.

Ureases are metalloenzymes found in plants, algae, fungi, and bacteria that are responsible for hydrolyzing urea into carbamate and ammonia. The bacterium Helicobacter pylori, which is associated with gastrointestinal disorders, produces large amounts of urease to neutralize stomach acidity. The rising antibiotic resistance of H.

Continuous collection of human mesenchymal-stromal-cell-derived extracellular vesicles from a stirred tank reactor operated under xenogeneic-free conditions for therapeutic applications.

Background: Mesenchymal-stromal-cell-derived extracellular vesicles (MSC-EVs) play a key role in the paracrine effects of MSC and have demonstrated therapeutic potential in various preclinical models. However, clinical translation is hindered by manufacturing practices relying on planar culture systems, fetal bovine serum (FBS)-supplemented media, and non-scalable, low-purity EV isolation methods that fail to meet dose and safety requirements, underscoring the need for innovative approaches. In this study, we developed a scalable platform to manufacture human MSC-EVs at clinically relevant numbers, integrating continuous collection of EV-enriched conditioned media (CM) using a stirred-tank reactor (STR) under xenogeneic-free conditions and a scalable downstream process.

Circulating microRNA profiles are associated with acute pain and stress in castrated and tail docked lambs.

Maintaining animal welfare is an essential component of animal production systems. However, multiple measurements are required to inform an animal's welfare state as there are currently no universal measurement tools. Novel biomarkers are increasingly being explored as measures of stress, pain and disease status in livestock.

Association Between Lupus Nephritis and Renal Clear-Cell Carcinoma: A Case Report and Review of the Literature.

Systemic lupus erythematosus (SLE) is an autoimmune disease affecting several organs, including the kidneys, potentially leading to lupus nephritis (LN). SLE has also been associated with several neoplasias, but its relation to renal cell carcinoma (RCC) has been little explored. We report a young women diagnosed concomitantly with LN and RCC.

Molluscicidal and Schistosomicidal Activities of 2-(1-Pyrazol-1-)-1,3,4-thiadiazole Derivatives.

Schistosomiasis is caused by flatworms of the genus , for which mollusks of the genus are intermediate hosts. Niclosamide (NCL) is a molluscicide recommended by the World Health Organization (WHO) for control of . Although effective, it is expensive and environmentally toxic, which raises concerns regarding its widespread use.

Beam data commissioning, validation and clinical implementation of SRS gyroscopic system.

Purpose: The very unconventional design and features of the new gyroscopic radiosurgery ZAP-X® system (ZAP Surgical Systems) generates a challenge for beam data acquisition, characterization and validation. Treatment Planning System commissioning was performed with different detectors, filling the absence of methodologies and correction factors published in international guidelines. The congruence of detectors response was compared with corresponding SciMoCa Monte Carlo (MC) synthetic data (Scientific RT GmbH).

XGBMUT: Predicting the Functional Impact of Missense Mutations Using an Extreme Gradient Boost Classifier.

Millions of new mutations have been discovered largely due to advancements in genome projects, but characterizing their effects through traditional wet-lab experiments remains labor-intensive and time-consuming. Functional prediction algorithms offer a solution by enabling the efficient screening of mutations, thereby saving time and resources. The objective of this study was to develop a competitive algorithm for predicting the functional impact of missense mutations.

Proximity proteomics reveals a mechanism of fatty acid transfer at lipid droplet-mitochondria- endoplasmic reticulum contact sites.

Membrane contact sites between organelles are critical for the transfer of biomolecules. Lipid droplets store fatty acids and form contacts with mitochondria, which regulate fatty acid oxidation and adenosine triphosphate production. Protein compartmentalization at lipid droplet-mitochondria contact sites and their effects on biological processes are poorly described.

Assessing the predicted impact of single amino acid substitutions in MAPK proteins for CAGI6 challenges.

New thermodynamic and functional studies have been recently conducted to evaluate the impact of amino acid substitutions on the Mitogen Activated Protein Kinases 1 and 3 (MAPK1/3). The Critical Assessment of Genome Interpretation (CAGI) data provider, at Sapienza University of Rome, measured the unfolding free energy and the enzymatic activity of a set of variants (MAPK challenge dataset). Thermodynamic measurements for the denaturant-induced equilibrium unfolding of the phosphorylated and unphosphorylated forms of the MAPKs were obtained by monitoring the far-UV circular dichroism and intrinsic fluorescence changes as a function of denaturant concentration.

In silico evaluation of N-aryl-1,10-phenanthroline-2-amines as potential inhibitors of T. cruzi GP63 zinc-metalloprotease by docking and molecular dynamics simulations.

Based on the in vitro trypanocidal efficacy of previously synthesized N-aryl-1,10-phenanthroline-2-amines (Phen1-20) (aryl = R-phenyl, 1- or 2-naphthyl), we explored the potential interactions of these derivatives as ligands of our comparative model of T. cruzi GP63 (TcGP63). This surface metalloprotease plays a crucial role in parasite adhesion to host cells and aids in cell invasion during T.

Synthesis and Structure-Activity Relationship of Thiourea Derivatives Against .

Leishmaniasis, caused by protozoa and transmitted by vectors, presents varied clinical manifestations based on parasite species and host immunity. The lack of effective vaccines or treatments has prompted research into new therapies, including thiourea derivatives, which have demonstrated antiprotozoal activities. We synthesized two series of ,'-disubstituted thiourea derivatives through the reaction of isothiocyanates with amines.

In vitro assays identified thiohydantoins with anti-trypanosomatid activity and molecular modelling studies indicated possible selective CYP51 inhibition.

This work investigates the anti-trypanosomal activities of ten thiohydantoin derivatives against the parasite Trypanosoma cruzi. Compounds with aliphatic chains (THD1, THD3, and THD5) exhibited the most promising IC against the epimastigote form of T. cruzi.

Assessing the predicted impact of single amino acid substitutions in calmodulin for CAGI6 challenges.

Recent thermodynamic and functional studies have been conducted to evaluate the impact of amino acid substitutions on Calmodulin (CaM). The Critical Assessment of Genome Interpretation (CAGI) data provider at University of Verona (Italy) measured the melting temperature (T) and the percentage of unfolding (%unfold) of a set of CaM variants (CaM challenge dataset). Thermodynamic measurements for the equilibrium unfolding of CaM were obtained by monitoring far-UV Circular Dichroism as a function of temperature.