The main goal of this study was to evaluate the potential of four yeasts-Rhodotorula mucilaginosa CCT 7688, Sporidiobolus pararoseus CCT 7689, Pichia fermentans CCT 7677, and Phaffia rhodozyma NRRL Y-17268-to produce carotenoids using soybean molasses as the sole nutrient source. Initially, they were cultivated in the medium-containing soybean molasses (C1, 34.32 g L) and compared to the standard medium Yeast Malt (YM).
View Article and Find Full Text PDFBioprocess Biosyst Eng
April 2022
This study focuses on the potential of Rhodotorula mucilaginosa CCT 7688 in simultaneous production of lipids and carotenoids in agroindustrial byproduct-based media and specially aims at establishing a process condition that guarantees high concentrations of both bioproducts, i.e., a carotenoid-rich microbial oil with potential economic value and health benefits attributed to carotenoids and fatty acids.
View Article and Find Full Text PDFBiotechnol Lett
July 2021
In the present study, a new extracellular polysaccharide (EPS-M816) was obtained during the growth of Mesorhizobium loti Semia 816 on a crude glycerol-based medium. EPS-M816 precipitate mainly consisted of carbohydrates (82.54%) and proteins (11.
View Article and Find Full Text PDFSince carotenoids are synthesized inside the cell, it is desirable to find an efficient method to extract carotegenic pigments. This study aimed at comparing the effectiveness of different chemical and mechanical techniques to disrupt the cell wall of and yeasts isolated from environmental samples. Among the techniques under study, the ultrasonic bath and the abrasion with glass beads yielded the most promising results for (84.
View Article and Find Full Text PDFBraz J Microbiol
April 2012
The work herewith investigated the production of yeast biomass as a source of protein, using Yarrowia lipolytica NRRL YB-423 and raw glycerol from biodiesel synthesis as the main carbon source. A significant influence of glycerol concentration, initial pH and yeast extract concentration on biomass and protein content was observed according to the 2v (5-1) fractional design. These factors were further evaluated using a central composite design and response surface methodology, and an empirical model for protein content was established and validated.
View Article and Find Full Text PDFThis paper presents the evaluation of some important parameters for the purification of phycocyanin using ion exchange chromatography. The influences of pH and temperature on the equilibrium partition coefficient were investigated to establish the best conditions for phycocyanin adsorption. The equilibrium isotherm for the phycocyanin-resin system was also determined.
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