Risk Factors and Prevalence of spp. in Poultry Carcasses in Slaughterhouses Under Official Veterinary Inspection Service in Brazil.

Because of the relevance of spp. in poultry, in 2016, the Brazilian Ministry of Agriculture, Livestock, and Supply updated its regulation for the control and monitoring of in the poultry meat production chain. We herein present the results of the official monitoring of sampling cycles of carcasses performed by the Brazilian official veterinary inspection service (SIF) and the Center for Epidemiology, Statistics, and Public Health of the Veterinary School of the Federal University of Minas Gerais.

KpSC-ID: a multiplex real-time PCR assay for the simultaneous detection of the species complex and specific identification of , and .

The species complex (KpSC) comprises five closely related bacterial species, namely , , , and . The KpSC is ubiquitous in the environment and is also an important human pathogen, particularly associated with healthcare-associated infections. The accurate detection and differentiation of the KpSC is challenging owing to the close phenotypic and genotypic identity (93-95% average nucleotide identity) shared between these members.

Prevalence of Eimeria spp. in goats from northern Paraná, Brazil.

This study aimed to evaluate the presence, identify Eimeria species, and epidemiological aspects associated with the infection in goat herds in northern Paraná, Brazil. A total of 384 fecal samples were collected from goats of different breeds, ages, and sexes from eight farms. An epidemiological questionnaire about rearing system (semi-intensive or intensive), age of the animals (up to 6 months or over 6 months), and type of floor in the pen (dirt or slatted) was used to evaluate the epidemiological aspects associated with Eimeria spp.

Accurate genotyping of three major respiratory bacterial pathogens with ONT R10.4.1 long-read sequencing.

High-throughput massive parallel sequencing has significantly improved bacterial pathogen genomics, diagnostics, and epidemiology. Despite its high accuracy, short-read sequencing struggles with the complete genome reconstruction and assembly of extrachromosomal elements such as plasmids. Long-read sequencing with Oxford Nanopore Technologies (ONT) presents an alternative that offers benefits including real-time sequencing and cost efficiency, particularly useful in resource-limited settings.

Clinical characteristics of cases during the 2024 pertussis epidemic in France, January 2024 to December 2024.

The French national surveillance of pertussis consists of several units, including general practitioners (GPs). Here, we report on the clinical characteristics of cases from January to December 2024 from primary care surveillance in France. During this period, a total of 689 pertussis cases were reported by GPs participating in the surveillance.

Combination of gold nanoparticles with near-infrared light as an alternative approach for melanoma management.

Melanoma is the most aggressive type of skin cancer and recently approved drugs are often associated with resistance and significant adverse effects. Therefore, the design of more effective and safe options remains imperative. Photothermal therapy (PTT) using gold nanoparticles (AuNPs) presents a promising and innovative approach.

Triplex real-time PCR ZKIR-T assay for simultaneous detection of the species complex and identification of sensu stricto.

Unlabelled: species complex (KpSC) members, including the most important species (phylogroup Kp1 of the KpSC), are important opportunistic pathogens that display increasing rates of antimicrobial resistance worldwide. As they are widespread in food and the environment, there is a need for fast, sensitive, and reliable methods to detect KpSC members in complex matrices. Previously, the ZKIR real-time PCR assay was developed to detect all KpSC members without distinction.

Diversity, functional classification and genotyping of SHV β-lactamases in .

Interpreting the phenotypes of alleles in genomes is complex. Whilst all strains are expected to carry a chromosomal copy conferring resistance to ampicillin, they may also carry mutations in chromosomal alleles or additional plasmid-borne alleles that have extended-spectrum β-lactamase (ESBL) activity and/or β-lactamase inhibitor (BLI) resistance activity. In addition, the role of individual mutations/a changes is not completely documented or understood.

Women's mental health and COVID-19: increased vulnerability and inequalities.

Introduction: The impact of COVID-19 on mental health has become a relevant object of research. Studies have demonstrated that women have experienced greater mental health challenges, highlighting the importance of public health systems to address women's specific needs.

Methods: This literature review explores the effects of the coronavirus pandemic on psychological distress among women, aiming to provide a comprehensive understanding of the subject and to explore how these research findings can guide public mental health care responses in crisis settings.

Resurgence of , including one macrolide-resistant isolate, France, 2024.

As other European countries, France is experiencing a resurgence of pertussis in 2024. Between 1 January and 31 May 2024, 5,616 (24.9%) positive qPCR tests were identified, following a 3-year period of almost null incidence.

Transient reemergence of in France in 2022.

Between March and October 2022, a peak of detection of by qPCR, real-time PCR was observed in France. Whether this peak was due to resurgence from previous circulating lineages or reintroduction into the country was unknown. The objective of this study is to understand transient increase observed in France in 2022 whereas it had virtually stopped being reported since the start of the COVID-19 pandemic in 2020.

Medication use for the management of professional performance: between invisibility and social normalisation.

This article aims to explore pharmaceuticalisation processes in professional work contexts. The approach focuses on identifying patterns of medicine and dietary supplement use for managing work performance, and on discussing the relationship between these consumption practices and work-related pressure factors. This analysis adapts the notions of 'normalisation' to understand the extent of cultural acceptability of these practices, and the notion of 'differentiated normalisation' to capture the tension between the trend towards normalisation of such consumption and its partial social (in)visibility within work settings.

Implementing a contextually appropriate foot-ankle exercise programme in primary care for the prevention of modifiable risk factors for ulcers in people with diabetes: protocol for a hybrid type 2 study.

Introduction: Diabetes is a highly prevalent disease that negatively impacts people's health and quality of life. It can result in diabetic peripheral neuropathy (DPN) and foot complications, which in turn lead to ulcers and amputations. The international guidelines on diabetic foot included specific foot-ankle exercises as preventive strategy capable of modifying the risk factors for ulcers.

Insights on trends in the management of metrological analysis of devices in blood and transfusion medicine services: Verification based on their fitness for purpose and suitability for their respective applications.

In the field of blood and transfusion medicine, European Directorate for the Quality of Medicines & HealthCare recommends practices for metrological devices. Monitoring and measurement resources, as well as their permitted metrological limits, must be considered in this regard. We must understand variables such as metrological domains, the International System of Units, metrological traceability, and metrological requirements and controls in the field of transfusions in order to understand these metrological devices.

Life cycle assessment of pharmaceutical packaging addressing end-of-life alternatives.

Pharmaceutical packaging waste has increased due to a higher global demand for pharmaceutical products, leading to more waste generation and associated environmental impacts. The main goal of this article is to present a cradle-to-grave life cycle assessment of pharmaceutical packaging, evaluating end-of-life (EoL) alternatives, aiming to identify hotspots and opportunities for improvement. A life cycle model was implemented for three types of pharmaceutical packaging (blisters, sachets, bottles; 23 packaging).

Medication literacy and its social contextuality.

This article aims to contribute to the discussion about medication literacy, by focussing on the social contextuality of the information mobilised in the use of medicines. We aim to explore the social construction processes of medication literacy, as an essential dimension for a more layperson-centred approach in the promotion of literacy in this field. This approach is justified by the growing social and cultural dissemination of medication use, the diversification of its uses beyond health and illness, and the increasing degree of lay autonomy in managing its use.

Antibiotics in Catalan Primary Care: Prescription, Use and Remedies for a Crisis of Care.

Antimicrobial resistance is one of the twenty-first century's major health challenges. Linked to the extensive use of antibiotics and other antimicrobials, resistance occurs when microbes stop responding to medications. Rates of antibiotic consumption in Spain are among the highest in Europe.

Genomic features of Klebsiella isolates from artisanal ready-to-eat food production facilities.

Increasing reports on K. pneumoniae strains with antimicrobial resistance and virulence traits from food and farm animals are raising concerns about the potential role of Klebsiella spp. as a foodborne pathogen.

Pituitary Apoplexy in Pregnancy: What do We Know?

Pituitary apoplexy refers to a rare clinical syndrome consisting of signs and symptoms that occur due to rapid expansion of the contents of the . It can occur spontaneously or associated with pituitary tumors. It can have a broad clinical spectrum, but usually presents with severe headache, visual impairment and hypopituitarism.