Lymphedema Is Severely Underdiagnosed in the Arthroplasty Clinic Population: A Bioimpedance Investigation.

Background: Lymphedema is a recognized risk factor for postoperative complications after total joint arthroplasty (TJA). Edema from sources other than lymphedema can also contribute to postoperative complications, as preoperative leg edema is known to worsen postoperatively, prolonging recovery and increasing complication risks. Current evidence surrounding lymphedema in the arthroplasty context is scarce, and historical barriers have hindered its diagnosis.

R-loop-induced irreparable DNA damage evades checkpoint detection in the C. elegans germline.

Accumulation of DNA-RNA hybrids in the form of R-loops can result in replication-transcription conflict that leads to the formation of DNA double strand breaks (DSBs). Using null mutants for the two Caenorhabditis elegans genes encoding for RNaseH1 and RNaseH2, we identify novel effects of R-loop accumulation in the germline. R-loop accumulation leads, as expected, to replication stress, followed by the formation of DSBs.