The combination of FLI, ITS, pFF, and citrate improves the developmental potential of gilt oocytes during in vitro maturation.

The intrinsic quality of porcine oocytes is a critical determinant of early embryonic development during in vitro production (IVP). This study investigated the effects of chemically defined in vitro maturation (IVM) systems supplemented with glutamine, GlutaMAX, insulin-transferrin-selenium (ITS), porcine follicular fluid (pFF), citrate, and growth factors (FGF2, LIF, IGF1, termed FLI) on the cumulus diffusion and maturation of oocytes derived from different diameters follicles. Moreover, four IVM media were systematically assessed for their effects on oocyte nuclear and cytoplasmic maturation and subsequent embryonic development.

Deletion of the mitochondrial calcium uniporter in adipose tissue promotes energy expenditure and alleviates diet-induced obesity.

Objective: Studies have shown a correlation between obesity and mitochondrial calcium homeostasis, yet it is unclear whether and how Mcu regulates adipocyte lipid deposition. This study aims to provide new potential target for the treatment of obesity and related metabolic diseases, and to explore the function of Mcu in adipose tissue.

Methods: We firstly investigated the role of mitoxantrone, an Mcu inhibitor, in the regulation of glucose and lipid metabolism in mouse adipocytes (3T3-L1 cells).

Mitoxantrone alleviates hepatic steatosis induced by high-fat diet in broilers.

Metabolic-dysfunction-associated fatty liver disease (MAFLD) is a common nutritional metabolic disease in poultry that seriously compromises the health of chickens and reduces the economic benefits of the industry. In this study, we investigated the therapeutic effect of mitoxantrone (MTX) on hepatic steatosis in broilers. We constructed a steatosis cell model in vitro by adding oleic acid and palmitic acid to chicken hepatocytes (LMH cells), to examine influence of MTX on fat deposition on LMH cells.

Dietary supplementation with perillartine ameliorates lipid metabolism disorder induced by a high-fat diet in broiler chickens.

Lipid metabolism disorders affect the growth and jeopardize the health of poultry, thus, decreasing economic benefits. Perillartine, a sweetener derived from Perilla frutescens, has excellent potential in regulating lipid metabolism. In this study, we explored the effects of perillartine on lipid metabolism in broiler chickens by establishing a nonalcoholic fatty liver model induced by a high-fat diet.