Highly efficient gene editing of using CRISPR/Cas9 combined with FACS.

() is a major causative agent of feline viral rhinotracheitis and ocular lesions. Due to its large DNA genome, the construction of recombinant viruses presents considerable challenges for conventional methodologies. In this study, we implemented an integrated strategy combining CRISPR/Cas9-mediated gene editing with fluorescence-activated cell sorting (FACS) to enable the rapid and efficient generation of recombinant viruses.

Impact of emergence delirium on long-term survival in older patients after major noncardiac surgery: A longitudinal prospective observational study.

Study Objective: To test the hypothesis that emergence delirium might be associated with worse long-term survival.

Design: A longitudinal prospective observational study.

Setting: A tertiary hospital in Beijing, China.

The glycosylation at positions 652 and 661 of viral spike protein negatively modulates porcine deltacoronavirus entry.

-glycosylation is a highly conserved glycan modification that plays crucial roles in various physiological processes, including protein folding, trafficking, and signal transduction. Porcine deltacoronavirus (PDCoV) poses a newly emerging threat to the global porcine industry. The spike protein of PDCoV exhibits a high level of -glycosylation; however, its role in viral infection remains poorly understood.