Publications by authors named "Bijay Shakya"
Objective: The mandibular condylar cartilage (MCC) of rats was examined with polarised light microscopy (PLM) to study the effects of ageing, oestrogen level, and altered dietary loading on the structure of the MCC.
Materials And Methods: 96 Sprague-Dawley rats were separated into 12 groups based on their age (5 months [young] and 14 months [old]), oestrogen status (ovariectomised [OVX], non-ovariectomised [non-OVX]), and diet (hard, normal, or soft). The MCC specimens were examined using PLM to evaluate the orientation and retardation of collagen fibrils.
Prevalence and classification of meniscal calcifications in the human knee.
Osteoarthritis Cartilage
November 2024
Article Synopsis
- The study aimed to explore meniscal calcifications in individuals with and without knee osteoarthritis (OA), focusing on two types: basic calcium phosphate (BCP) and calcium pyrophosphate dihydrate (CPP).
- The researchers analyzed 82 meniscal samples from 41 subjects, comparing those with OA (who had knee replacements) to deceased donors without OA, using histological methods and Raman spectroscopy for detailed analysis.
- Results showed that all OA participants had some calcifications, primarily BCP, while CPP was more common in donor samples, indicating that BCP may play a key role in the OA process deserving further study.
Article Synopsis
- The study examines the presence and reduction of SARS-CoV-2 in various water samples from two wastewater treatment plants in Nepal between July 2020 and February 2021, finding significant detection rates.
- SARS-CoV-2 RNA was found in a high percentage of samples from wastewater treatment plants, river water, hospital wastewater, and sewer lines, indicating the biological treatments used are insufficient for complete RNA elimination.
- The research suggests wastewater-based epidemiology (WBE) can be an effective tool for monitoring COVID-19 trends, especially in developing countries, despite some limitations in correlating RNA concentrations with new COVID-19 cases.
The use of Fourier Transform Infrared (FTIR) microspectroscopy to study cancerous cells and tissues has gained popularity due to its ability to provide spatially resolved information at the molecular level. Transmission and transflection are the commonly used measurement modes for FTIR microspectroscopy, and the tissue samples measured in these modes are often paraffinized or deparaffinized. Previous studies have shown that variability in the spectra acquired using different measurement modes and sample processing methods affect the result of the analysis.
View Article and Find Full Text PDFDiscrimination of melanoma cell lines with Fourier Transform Infrared (FTIR) spectroscopy.
Spectrochim Acta A Mol Biomol Spectrosc
June 2021
Among skin cancers, melanoma is the lethal form and the leading cause of death in humans. Melanoma begins in melanocytes and is curable at early stages. Thus, early detection and evaluation of its metastatic potential are crucial for effective clinical intervention.
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