Publications by authors named "Bernardo Predicala"

Air pollutants, such as particulate matter (PM) and ammonia (NH), generated by intensive animal farming pose considerable threats to human health, animal welfare, and ecological balance. Conventional materials are often ineffective at simultaneously removing multiple pollutants, maintaining a low pressure drop, and ensuring durability in heavily polluted environments. Inspired by the dust-retention properties of (PS) needles, this study developed a biomimetic grooved ribbon fiber using electrospinning technology.

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The growing concern over the emergence of antimicrobial resistance (AMR) in animal production as a result of extensive and inappropriate antibiotic use has prompted many swine farmers to raise their animals without antibiotics (RWA). In this study, the impact of implementing an RWA production approach in sow barns on actual on-farm antibiotic use, the emergence of AMR, and the abundance of pathogens was investigated. Over a 13-month period, fecal and nasopharynx samples were collected at 3-month intervals from sows raised in RWA barns and sows in conventional barns using antibiotics in accordance with the new regulations (non-RWA).

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Methods to control microbial contamination in confined livestock facilities are important to the health of both animals and workers. In addition, bacterial contamination is also a food safety issue. The most common disinfection technique employed in livestock facilities is the application of oxidizing agents (e.

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In response to new stringent regulations in Canada regarding the use of antibiotics in animal production, many farms have implemented practices to produce animals that are raised without antibiotics (RWA) from birth to slaughter. This study aims to assess the impact of RWA production practices on reducing the actual total on-farm use of antibiotics, the occurrence of pathogens, and the prevalence of antimicrobial resistance (AMR). A 28-month longitudinal surveillance of farms that adopted the RWA program and conventional farms using antibiotics in accordance with the new regulations (non-RWA) was conducted by collecting fecal samples from 6-week-old pigs and composite manure from the barn over six time points and applying whole-genome sequencing (WGS) to assess the prevalence of AMR genes as well as the abundance of pathogens.

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Article Synopsis
  • The project assessed the effects of high wheat millrun diets, with added multi-carbohydrase enzymes, on growing pigs' nutrient digestibility, growth performance, and greenhouse gas emissions.
  • Feeding pigs diets with increasing amounts of wheat millrun (up to 30%) led to lower energy, nitrogen, and phosphorus digestibility, as well as reduced average daily gain and gain-to-feed ratio.
  • The addition of enzymes had little impact on digestibility, performance, or greenhouse gas emissions, indicating that while wheat millrun affected certain nutritional parameters, it did not contribute to increased emissions.
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Extended exposure to airborne contaminants such as ammonia (NH) and respirable dust in swine facilities has been associated with various health problems among swine barn workers. The overall goal of this study was to assess the impact of selected control measures, namely, canola oil sprinkling, low crude protein diet, high level of cleaning, and manure pH manipulation, on NH and respirable dust concentrations in swine production rooms through area sampling and on worker exposure to these contaminants in accordance with National Institute of Occupational Safety and Health (NIOSH) methods for occupational exposure monitoring. Results from five trials showed that low crude protein diet can be used for reducing worker exposure to NH while oil sprinkling can be used for controlling respirable dust levels in swine rooms.

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New Canadian regulations have required that all use of antibiotics in livestock animal production should be under veterinary prescription and oversight, while the prophylactic use and inclusion of these agents in animal feed as growth promoters are also banned. In response to this new rule, many Canadian animal producers have voluntarily implemented production practices aimed at producing animals effectively while avoiding the use of antibiotics. In the swine industry, one such program is the 'raised without antibiotics' (RWA) program.

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The passive flux sampling is an economic and easy way to estimate gas emissions from agriculture sources. In the last decade, specific passive flux samplers (PFSs) have been developed to estimate nitrous oxide (NO) emissions from agriculture sources. Packed with silica gel and zeolite 5A, the PFSs were placed facing the emission source direction close to the ventilation shafts.

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Article Synopsis
  • Extended exposure to noise and airborne contaminants in swine barn workers is linked to health issues, and this study aimed to monitor actual exposure levels during their daily tasks.
  • Selected workers were equipped with personal monitoring systems to track respirable dust, ammonia, hydrogen sulfide, and noise exposure over 50 monitoring days across different seasons.
  • While average exposure levels for these hazards were generally below safety limits, specific tasks like feeding pigs and draining manure posed significant risks, exceeding safe thresholds for respirable dust and gases; implementing control measures and protective equipment can help reduce these occupational exposure risks.
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Intensification of modern swine production has led to many new technologies, including needleless injectors. Although needleless injectors may increase productivity (by reducing injection time) and reduce needlestick injuries, the effect on risk for musculoskeletal disorders is not clear. This project will compare conventional needles with needleless injectors in terms of cost, productivity, injury rates, biomechanical exposures, and worker preference.

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Phenol biodegradation was evaluated in batch and continuous flow microbial fuel cells (MFCs). In batch-operated MFCs, biodegradation of 100-1000 mg L phenol was four to six times faster when graphite granules were used instead of rods (3.5-4.

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In this study, we tested the antimicrobial activity of three metal nanoparticles (NPs), ZnO, MgO, and CaO NPs, against Salmonella enterica serovar Enteritidis in liquid medium and on solid surfaces. Out of the three tested metal NPs, ZnO NPs exhibited the most significant antimicrobial effect both in liquid medium and when embedded on solid surfaces. Therefore, we focused on revealing the mechanisms of surface-associated ZnO biocidal activity.

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Biodegradations of Na-lactate and Na-acetate were evaluated in microbial fuel cell (MFC) type bioreactors. Increase in lactate concentration from 1,000 to 5,000 mg L(-1) enhanced the biodegradation rate from 4.6 to 23.

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The effects of manure age on emission of H(2)S and required level of nitrite or molybdate to control these emissions were investigated in the present work. Molybdate mediated control of H(2)S emission was also studied in semi-pilot scale open systems, and in specifically designed chambers which simulated swine production rooms. With fresh 1-, 3- and 6-month old manures average H(2)S concentration in the headspace gas of the closed systems were 4856+/-460, 3431+/-208, 1037+/-98 ppm and non-detectable, respectively.

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The aim of this work is to better understand the responses of people that are exposed to agricultural organic dust and other factors in modern swine production. We investigated the effects of toll-like receptor 4 (TLR4) genotype and gender on respiratory responses of naïve volunteers (18-28 years) to swine barn exposure. Non-smoking healthy subjects (16 men and 13 women) with TLR4 299 (Asp299Gly) and/or 399 (Thr399Ile) polymorphisms (TLR4 299/399) and age-sex matched subjects with TLR4 wild-type alleles spent 5 h in a nonexposed environment (baseline day) and 5 h in a swine facility (exposure day).

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Article Synopsis
  • TLR4 variants may lead to decreased respiratory responses to inhaled endotoxins, particularly in naive subjects.
  • Research involved comparing 29 individuals with TLR4 polymorphisms to 29 wild-type controls, observing their respiratory function after exposure to high endotoxin levels.
  • Results showed that those with TLR4 variants experienced less reduction in lung function indicators (like FEV1) compared to wild-type participants, suggesting a protective effect of these genetic variants at higher concentrations of endotoxins.
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The objective of this study was to obtain diurnal variation profiles of odor and gas (ammonia [NH3], hydrogen sulfide [H2S], carbon dioxide [CO2]) concentrations and emission rate (OGCER) from confined swine grower/ finisher rooms under three typical weather conditions (warm, mild, and cold weather) in a year. Two grower/ finisher rooms, one with a fully slatted floor and the other with partially slatted floors, were measured for 2 consecutive days under each weather condition. The results revealed that the diurnal OGCER in the room with a fully slatted floor was 9.

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Biogenic production of hydrogen sulphide (H2S) in oil reservoirs (souring) has been shown to be controlled effectively using nitrite and molybdate salts. In the present work the effects of addition of nitrite and molybdate on reducing the emission of H2S from swine manure slurry was investigated in the laboratory and semi-pilot scale systems. Addition of 80 mM nitrite or 2 mM molybdate (final concentration in the manure slurry) to fresh manure in the laboratory scale closed systems (125 mL and 4 L) reduced the concentration of H2S in the headspace gas from 1500 microL L(-1) to 10 microL L(-1) which maintained during the remaining period of trials (40-60 days).

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Article Synopsis
  • Researchers compared inhalable and total dust sampling devices for measuring airborne dust in swine confinement buildings, analyzing data from three facilities (n = 77 pairs).
  • The study found that the overall geometric mean concentration for the IOM sampler (1.18 mg/m³) was significantly higher than that of the TCF sampler (1.08 mg/m³), and a regression analysis provided a conversion factor for estimating TCF values based on IOM measurements.
  • Additional comparisons of different dust samplers indicated significantly higher concentrations from the IOM compared to the CIS, while no significant differences were observed among the other sampler pairs, suggesting a need for work-specific conversion coefficients for total dust exposure assessments.
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Bioaerosol concentrations inside one naturally ventilated and one mechanically ventilated swine finishing barn were assessed by sampling air using membrane filtration and impaction (six-stage Andersen sampler), and assayed by culture method. The barns, located on the same commercial farm in northeast Kansas, did not show any significant difference (p > 0.05) in concentrations of total and respirable airborne microorganisms.

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