Anti-Inflammatory Activity of Sarcoaldosterol A Isolated From Heteroxenia fuscescens: Insights From In Vitro, In Silico, and Spectroscopic Studies.

Chronic inflammation is a key factor in the progression of many diseases, driving ongoing efforts to identify new and effective anti-inflammatory agents. Sarcoaldosterol A, a polyhydroxylated gorgostane steroid isolated from Heteroxenia fuscescens, was evaluated for its anti-inflammatory activity using in vitro studies, molecular docking, and quantum chemical calculations. Its structure was elucidated on the basis of 1D NMR, HR-ESI-MS, and comparison with literature.

Ajwa date extract (): Phytochemical analysis, antiviral activity against herpes simplex virus-I and coxsackie B4 virus, and in silico study.

Objectives: To investigate the phytochemical composition of Ajwa date extract and evaluate its antiviral activity and mechanism of action.

Methods: High perfomance liquid chromatography, gas chromatography-mass spectrometry, and liquid chromatography-mass spectrometry were used to analyze the phytochemical profile of Ajwa date extract. The antiviral activity was assessed using the MTT colorimetric assay against herpes simplex virus type I (HSV-I) and coxsackievirus B4 (CVB-4).

Assessing the prevalence and knowledge of anabolic steroid use in male athletes in Al Madina Al Munawara, Saudi Arabia.

Objectives: To assess the prevalence of anabolic steroid use and the level of knowledge on anabolic steroids among the male athletes in Al Madina Al Munawara, Saudi Arabia.

Methods: A cross-sectional study was conducted on male athletes randomly selected from the private athletic centers in Al Madina Al Munawara over 5 months. Data were collected from all participants using a self-administered anonymous questionnaire with 33 questions.

Unravelling the therapeutic landscape of bile acid-based therapies in gastrointestinal disorders.

Bile acids serve as endogenous ligands for nuclear and cell membrane receptors and play a crucial role in bile acid and lipid metabolism. These detergent-like compounds promote bile flow and aid in the absorption of dietary fats and fat-soluble vitamins in the intestine. Synthesized in the liver as end products of cholesterol catabolism, bile acids exhibit a chemical structure comprising a nucleus and a side chain featuring a carboxyl group, with diverse steric arrangements and potential polar substituents.

Identification of bile acid-CoA:amino acid N-acyltransferase as the hepatic N-acyl taurine synthase for polyunsaturated fatty acids.

N-acyl taurines (NATs) are bioactive lipids with emerging roles in glucose homeostasis and lipid metabolism. The acyl chains of hepatic and biliary NATs are enriched in polyunsaturated fatty acids (PUFAs). Dietary supplementation with a class of PUFAs, the omega-3 fatty acids, increases their cognate NATs in mice and humans.

Bile acid conjugation deficiency causes hypercholanemia, hyperphagia, islet dysfunction, and gut dysbiosis in mice.

Bile acid-CoA: amino acid N-acyltransferase (BAAT) catalyzes bile acid conjugation, the last step in bile acid synthesis. BAAT gene mutation in humans results in hypercholanemia, growth retardation, and fat-soluble vitamin insufficiency. The current study investigated the physiological function of BAAT in bile acid and lipid metabolism using Baat mice.