Spectroscopic Insights into the Localization and Photodynamic Efficacy of Aluminum Tetrasulfonated Phthalocyanine for Colorectal Cancer Therapy.

This article explores the potential of aluminum tetrasulfonated phthalocyanine (AlPcS) as a photosensitizer for photodynamic therapy (PDT) in colorectal cancer (CRC), utilizing Raman imaging, steady-state absorption and fluorescence spectroscopy in UV-Vis spectral region, and transient absorption spectroscopy. Our study demonstrates that in human colon cancer cells, the administered photosensitizer preferentially localizes to the endoplasmic reticulum and lipid droplets, mirroring its distribution in normal cells. Furthermore, the addition of DTAC significantly enhances the permeability of cell membranes to AlPcS, leading to an increased intracellular concentration of the photosensitizer, as evidenced by the elevated fluorescence intensity around 679 nm, even after just 30 min of incubation.

Exploring the Impact of Citalopram on Human Colon Cells: Insights into Antidepressant Action Beyond the Brain.

Depression is a common comorbidity among cancer patients, including those with colorectal cancer (CRC), often requiring the use of antidepressants such as citalopram, a selective serotonin reuptake inhibitor (SSRI). While citalopram is primarily prescribed for its central nervous system effects, its absorption through the gastrointestinal system raises questions about its direct impact on intestinal cells. This study investigates the dual effects of citalopram on healthy and cancerous colorectal cells to elucidate its potential role beyond mood regulation.

The effect of enantiomers of thalidomide on colon cells-Raman spectroscopy studies.

Enantiomers are molecules that have the same molecular formula but the atoms are linked differently. Differentiation between enantiomers is highly significant in scientific research, particularly in pharmaceuticals. This research demonstrates how conventional Raman spectroscopy and statistical methods can be applied to the analysis of enantiomers.

Hypoxic conditions by Raman microspectroscopy - Reprogramming of fatty acids and glucose metabolism during colon cancer progression.

Cellular respiration is the primary metabolic process for producing the energy (ATP) needed for survival. Disruptions in this process can lead to various diseases, including colon cancer. This paper reviews the current understanding of how excess fatty acids (FAs) and glucose (Glc) alter metabolic pathways.

Raman Imaging-A Valuable Tool for Tracking Fatty Acid Metabolism-Normal and Cancer Human Colon Single-Cell Study.

Altered metabolism of lipids is a key factor in many diseases including cancer. Therefore, investigations into the impact of unsaturated and saturated fatty acids (FAs) on human body homeostasis are crucial for understanding the development of lifestyle diseases. In this paper, we focus on the impact of palmitic (PA), linoleic (LA), and eicosapentaenoic (EPA) acids on human colon normal (CCD-18 Co) and cancer (Caco-2) single cells using Raman imaging and spectroscopy.