High GVHD Prevalence in Travel-Based SCT despite Young Patients and HLA Matching.

: Patients from regions without stem cell transplantation (SCT) facilities often seek treatment abroad and return home for post-transplant care. Although extensive data exist on graft-versus-host disease (GVHD) and its risk factors, information on international SCT patients returning to countries that lack transplant facilities and expertise is scarce and not well documented. : We screened 149 transplant recipients and analyzed the data of 91 patients who received transplants abroad and were followed up at our center from January 2019 to December 2022.

Antioxidant, Protein-Binding, and Copper(II) Ion Sensing Activities of Phytochemicals: An Integrated Experimental and Computational Study.

The genus Rhus, recognized for its wide range of biological activities, is a rich reservoir of bioactive phytochemicals. Chemical investigation of () leaves led to the isolation and characterization of two new compounds, 4-octadecylphenol (Ra-01) and 2-(7-hydroxy-2-oxo-2-chromen-4-yl)-2-methylpropanoic acid (Ra-02), alongside five known compounds: lupeol, dimethyl ester of terephthalic acid, ethyl gallate, friedelin, and oleanolic acid. The structures of all compounds were elucidated by using spectroscopic techniques (IR, UV, NMR, and mass spectrometry).

Polymorphism Analysis as Biomarker in Genes AIRE, CD40, HLA-DRB1 and TRAF1/C5 SNPs in Rheumatoid Arthritis Patients.

The main objective of the study was to determine the association of genes AIRE (rs2075876), CD40 (rs4810485), HLA-DRB1 (rs6457617) and TRAF1/C5 (rs10818488) polymorphisms with rheumatoid arthritis (RA) from the population of Pakistan. Blood samples of 300 RA patients and 300 healthy controls were taken from different hospitals in Pakistan. Extraction of DNA was carried out; a specific region of DNA was amplified using PCR.

Multifunctional ZnO Nanoparticles Synthesized Using Extract: Characterization, Photocatalytic Activity, Antimicrobial Assessment, and In Silico Modeling.

This study investigates the green synthesis of zinc oxide nanoparticles (ZnO NPs) using the aqueous extract of the aquatic plant (greater duckweed) and evaluates their multifunctional properties. The ZnO NPs were synthesized via a sustainable method and characterized using UV-visible spectroscopy, TEM, FESEM, EDX, FTIR, and XRD analyses. UV-visible spectroscopy confirmed the formation of ZnO NPs with a characteristic absorption peak at ∼349 nm.

Design and Development of Self-Emulsifying Drug Delivery System to Improve the Solubility and Bioavailability of Ciprofloxacin.

Ciprofloxacin, a potent fluoroquinolone antibiotic, is used to treat various bacterial infections. This drug has low aqueous solubility and limited oral bioavailability. To overcome these limitations, this study focused on developing a Self-Emulsifying Drug Delivery System (SEDDS) for Ciprofloxacin, aiming to enhance its solubility and bioavailability.

A comparative investigation of ultrasonication and magnetic stirring methods for green synthesis of zinc oxide nanoparticles using Punica granatum peels.

Green synthesis of nanoparticles is a safe and eco-friendly approach using plant materials as reducing and stabilizing agents for the synthesis of important nanoparticles. This study investigates the effect of ultrasonication and magnetic stirring methods on synthesis of zinc oxide nanoparticles. In this study the zinc oxide nanoparticles were synthesized by two methods Ultrasonication and magnetic stirring using Punica granatum (Pomegranate) fruit peels.

Investigation of molecular patterns present in RIPK1 and systemic approach to determine its differential expression in various brain parts.

Receptor-interacting serine/threonine-protein kinase 1 (RIPK1) regulates the pro-survival NF-κB signaling and death via necroptosis in response to various inflammatory and pro-death stimuli, leading to various human diseases. It is linked with the pathology of neuroinflammation and implicated in neurodegenerative disorders. Considering the imperative role of RIPK1 kinase in neuronal health, molecular patterns, effects of evolutionary forces on composition and codon bias, and tissue-wise expression pattern analysis in 12 different parts of the brain was done using a systematic approach in the RIPK1 kinase transcripts.

Evaluating the Dentin-Adhesive Interface of a Nanoparticle-Enhanced Universal Adhesive Using Scanning Electron Microscopy and Its Impact on μTBS and Degree of Conversion.

Evaluation of the effects of various nanoparticles (NPs), titanium dioxide (TiO), cerium dioxide (CeO), and copper oxide (CuO), when incorporated into universal adhesive (UA), on the micro tensile bond strength (μTBS), degree of conversion (DC), adhesive interface, and resin tag assessment. Before incorporating nanoparticles (NPs) into UA, elemental analysis was conducted on all NP types using energy-dispersive spectroscopy (EDS). The study involved 48 intact human molars, divided into four groups based on UA modification with different NPs: Group 1 (Control), Group 2 (TiONPs), Group 3 (CeONPs), and Group 4 (CuONPs).

Immunoinformatics-Guided Whole Proteome-Based Multi-Epitope mRNA Vaccine Design Against Nocardia asteroides Using Surface Antigens-A Subtractive Proteomics and Reverse Vaccinology Approach.

Nocardiosis is caused by the aerobic gram-positive bacterium Nocardia asteroides which is multidrug-resistant and primarily affects immunocompromised individuals. This study aims to design a broad-spectrum vaccine against Nocardiosis. We used subtractive proteomics and vaccinomics approaches to identify vaccine candidates to design an epitope-based vaccine against Nocardiosis.

In silico identification of PPARγ agonists from diffractaic acid analogs in prostate cancer: a comprehensive computational approach.

Unlabelled: Prostate cancer (PCa) is the second most frequent and the fifth greatest cause of death in men. Although there are already therapies for early-stage PCa, their effectiveness in advanced PCa is limited, primarily because of medication resistance or poor efficacy. To find new therapeutic indications or repurpose current medications, this project intends to use computational approaches to investigate possible anti-PCa compounds based on simulated screening of FDA-approved drug bank databases.

In Silico ADMET Studies, Molecular Docking and Molecular Dynamics Simulation of Thiadiazole Derivatives for the Identification of Putative HsaA Monooxygenase Inhibitors.

Introduction: The rise of drug-resistant strains of Mycobacterium tuberculosis (Mtb) represents a substantial public health challenge. Current TB treatments involve the combination of several antibiotics and other agents. However, the development of drug resistance, reduced bioavailability, and elevated toxicity have rendered most of the drugs less effective.

Whole proteome-integrated and vaccinomics-based next generation mRNA vaccine design against Pseudomonas aeruginosa-A hierarchical subtractive proteomics approach.

Pseudomonas aeruginosa (P. aeruginosa) is a multidrug-resistant opportunistic pathogen responsible for chronic obstructive pulmonary disease (COPD), cystic fibrosis, and ventilator-associated pneumonia (VAP), leading to cancer. Developing an efficacious vaccine remains the most promising strategy for combating P.

Whole genome analysis and biocontrol potential of endophytic Bacillus cereus EMS1 against Fusarium wilt in banana.

Endophytic bacteria are essential for promoting plant growth and increasing plant resilience to various environmental stresses. Although it is well-documented that several endophytic Bacillus species exhibit plant growth-promoting properties, this is the first report on the genome study of Bacillus cereus EMS1, isolated from Musa acuminata G9 in India. This study analyzed the genomics, plant growth traits, and fusarium wilt mitigation potential of Bacillus cereus EMS1.

Anxiety status in outpatients of Type-2 diabetes mellitus.

Objectives: This study intended to determine the frequency of anxiety in outpatients of Type-2 diabetes mellitus (T2DM) and to evaluate its associated characteristics.

Methods: This cross-sectional observational study was accomplished from July 1, 2023, to December 31, 2023, at the Department of Endocrinology at Hayatabad Medical Complex, Peshawar. The study comprised of individuals with T2DM who were at least 18 years of age and of either gender and who presented to the outpatient department for various reasons.

Insights into genetic architecture and disease associations of genes associated with different human blood group systems using codon usage bias.

The differential use of synonymous codons of an amino acid is an imperative evolutionary phenomenon, termed codon usage bias, that functions across various levels of organisms. It is accustomed to providing an understanding of a gene's differential architecture driven by functional regulation of gene expression. Numerous synonymous mutations are linked to various diseases, demonstrating that silent mutations can be deleterious.

Repurposing of dipeptidyl peptidase FDA-approved drugs in Alzheimer's disease using network pharmacology and in-silico approaches.

Type 2 diabetes mellitus (T2DM) and Alzheimer's disease (AD) have similar clinical characteristics in the brain and islet, as well as an increased incidence with ageing and familial susceptibility. Therefore, in recent years there has been a great desire for research that elucidates how anti-diabetic drugs affect AD. This work attempts to first elucidate the possible mechanism of action of DPP-IV inhibitors in the treatment of AD by employing techniques from network pharmacology, molecular docking, molecular dynamic simulation, principal component analysis, and MM/PBSA.

Antimicrobial and Hepatoprotective Properties of Pods of (L.) Willd. ex Delile: and Approaches.

Background: is a multipurpose plant known for its remedial properties but the antimicrobial and hepatoprotective activity of its pods remained unexplored.

Objective: This study aimed to evaluate the antimicrobial and hepatoprotective activity of n-hexane (ANPH) and methanol (ANPM) extracts of pods to scientifically validate their medicinal claims.

Methods: After the pharmacognostic evaluation of pods, tests were carried out to estimate phenolic and flavonoid content and antimicrobial potential.

Formulation and evaluation of herbal essential oil based nail lacquer for management of onychomycosis.

Introduction: Onychomycosis (Paronychia) is a common fungal nail infection, characterised by thickened, discoloured and brittle nails often accompanied by pain and discomfort. It is one of the most prevalent fungal nail infections with the global incidence of >20 % affected commonly by Candida albicans and Candida parapsilosis. The market today is flooded with various antifungal therapies (Azoles, Imidazoles, Allylamines, etc.

Development and In Vitro Characterization of Azadirachta Indica Gum Grafted Polyacrylamide Based pH-Sensitive Hydrogels to Improve the Bioavailability of Lansoprazole.

The present study intended to develop a pH-responsive hydrogel based on Neem gum (Ng) to improve Lansoprazole (LSP) oral bioavailability. Azadirachta Indica seed extract was used to obtain Ng. pH-responsive hydrogel formulations (F1-F9) were prepared using different Ng ratios, Acrylamide (AAm), and methylene-bis-acrylamide (MBA).

Phytochemical investigation and spectral characterization of isolated compounds from (D. Don) M. Roem (syn. Roxb) leaves: evaluation of antioxidant activity and molecular docking analysis.

(D. Don) M. Roem (syn.

Flexible high electrochemical collagen/lignin composite hydrogel for sensing and supercapacitor applications.

Synthesis of polymer-based highly conductive hydrogels from natural and renewable sources with robust mechanical performances in flexible electronics remains a great challenge. In this research, a dynamic redox system is designed by using collagen (CL), sulfonate lignin (SL), acrylic acid (AA), and Al to synthesize CL/PAA/SL/Al hydrogels. The formation of effective complexes of Al with the abundant functional groups of CL, SL and PAA, the prepared hydrogel delivers various specific properties, for example, excellent ionic conductivity (4.

In-silico Screening and Synthesis of Benzo[c]Pyridazines Targeting N-Methyl D-Aspartate Glutamate Receptor for the Treatment of Seizures.

This study focuses on synthesizing novel benzopyridazine compounds with an evaluation of their anti-epileptic activity by in-silico screening and MES test. The compounds were synthesized under controlled conditions by the reaction of the substituted anilines with sodium nitrite, followed by the reaction with cyanoacetamide, substituted urea, ethanol, and water. The final compounds (5a-d; 6a-d) were tested for antiepileptic activity by in-silico screening targeting N-Methyl D-Aspartate glutamate receptor (PDB ID:5IPV).