Exploring the Homeopathic Therapeutic Potential of Acorus calamus: A Multi-center Open-Label Clinical Verification Study.

Clinical verification in homeopathy is a systematic process that aims to validate the therapeutic efficacy of homeopathic medicines. The Central Council for Research in Homoeopathy (CCRH) has been conducting clinical verification studies in lesser known, fragmentarily proved and rare medicines such as through its Clinical Verification Program, thereby expanding the therapeutic utility of these medicines.The primary objective of the study was to assess the change in intensity of presenting symptoms of the participants after administration of the medicine by measuring change in Measure Yourself Medical Outcome Profile-2 (MYMOP-2) profile score.

Proximity to a hazardous waste thermal treatment facility alters human physiology: a community-driven pilot study.

Open burn/open detonation (OB/OD) disposes of explosive waste via uncontrolled combustion, releasing harmful pollutants like toxic gases and particulate matter. Colfax, Louisiana, houses the nation's only commercially OB/OD thermal treatment (TT) facility, raising concerns about environmental and public health impacts due to its emissions. In this exploratory pilot study, we investigated metabolic alterations indicative of potential health impacts from exposure to emissions from a TT facility through an untargeted metabolomics analysis of urine samples obtained from local residents.

Dual Disruption of Embryonic Angiogenesis by Hyperglycemia: Structural and Hemodynamic Alterations Revealed via OCT Angiography and Biospeckle Imaging in the Chick CAM Model.

Objective: This study investigates the effects of hyperglycemia on vascular morphology and hemodynamics during embryogenesis using the chick chorioallantoic membrane (CAM) model.

Methods: We employed a dual-modality, label-free imaging approach, Optical Coherence Tomography Angiography (OCTA) and biospeckle imaging, to evaluate microvascular architecture and real-time flow dynamics in chick embryos subjected to hyperglycemic conditions. Quantitative metrics such as vessel area, branching junctions, lacunarity, and biospeckle contrast were analyzed to assess angiogenic and metabolic responses.

Traffic-Emitted Amines Promote New Particle Formation at Roadsides.

New particle formation (NPF) is a major source of atmospheric aerosol particles, significantly influencing particle number concentrations in urban environments. High condensation and coagulation sinks at highly trafficked roadside sites should suppress NPF due to the low survival probability of clusters and new particles, however, observations show that roadside NPF is frequent and intense. Here, we investigate NPF at an urban background and roadside site in Central Europe using simultaneous measurements of sulfuric acid, amines, highly oxygenated organic molecules (HOMs), and particle number size distributions.

The impact of olezarsen on hypertriglyceridemia in high cardiovascular risk patients: a systematic review, meta-analysis, and meta-regression.

Background And Objective: Hypertriglyceridemia is a prevalent lipid disorder that considerably increases the risk of cardiovascular diseases, pancreatitis, and metabolic syndrome. Existing treatment options, including lifestyle changes and medications, often show limited effectiveness and may cause side effects. Olezarsen, an antisense oligonucleotide targeting apolipoprotein C-III (APOC3), represents a novel therapeutic strategy for lowering triglyceride levels.

Voluntary Activity Wheel Running Improves Hyperammonaemia-Induced Skeletal Muscle Molecular and Metabolic Perturbations in Mice.

Aim: Voluntary exercise improves clinical outcomes in healthy subjects, but increased muscle ammoniagenesis may limit beneficial responses during hyperammonaemia in chronic diseases. Responses to 4-weeks voluntary wheel running (VWR) were compared with usual activity (UA) to determine if hyperammonaemia alters VWR responses and if VWR alters muscle responses to hyperammonaemia.

Methods: Eight- to 10-week-old male C57BL/6J mice were treated with 6 weeks of subcutaneous infusion of 2.

A Computational Study on the Atmospheric Fate of Carbon-Centered Radicals from the 3-Methyl-2-butene-1-thiol + OH Reaction: Mechanistic Insights and Atmospheric Implications.

The reaction of 3-methyl-2-butene-1-thiol (MBT; (CH)C═CHCHSH) with the OH radical is reported to proceed via the addition to either of the sp hybridized C atoms, forming the two distinct C-centered radicals: (CH)C(OH)CHCHSH (R1) and (CH)CCH(OH)CHSH (R2). Understanding the fate of these radicals is important for elucidating MBT's atmospheric transformation mechanisms and the reaction products. Using quantum chemical calculations and kinetic modeling, we show that the unimolecular dissociation as well as isomerization reactions of R1 are kinetically unfavorable due to high energy barriers, and that R1 most likely reacts with atmospheric O to form R1O ((CH)C(OH)CH(OO)CHSH).

Mechanistic behavior of fenugreek α-amylase on starch hydrolysis upon interaction with Hesperetin and Luteolin probed by multispectroscopic and in silico approaches.

Fortification of food products with bioactive flavonoids could help regulate postprandial glucose levels, offering a natural and dietary approach to managing type 2 diabetes. Herein, the inhibitory effect of flavonoids Luteolin and Hesperetin on fenugreek α-amylase was investigated. Results demonstrated that Luteolin exhibited stronger inhibitory effect with IC₅₀ value of 85.

Identification of Botanicals Based on Their Chemical Barcode Using Ultraperformance Liquid Chromatography-Mass Spectrometry.

Rationale: Plants synthesize diverse secondary metabolites, often specific to particular species. These metabolites (phytochemicals) exhibit restricted distribution among certain plant families, genera, or species. Due to their species-specific characteristics, they can serve as chemical markers to identify and authenticate economically important botanicals.

Development of Edible Carbohydrate-Protein Sports Gels to Optimize the Muscle Glycogen Re-Synthesis.

This study was aimed at providing athletes a solution to replenish the muscle glycogen re-synthesis at an optimal rate with hemp seeds as a natural protein source and Bengal gram dal and its use for the preparation of gel. The gel contains the richest source of energy, and it is an effective way to provide energy and nutrients to athletes. The gel was prepared in three variations with different hemp seed concentrations.

Effects of music therapy on anxiety among patients undergoing cardiac procedures: a systematic review and meta-analysis.

Objective: The article aims to assess impact of music therapy on anxiety in patients undergoing cardiac procedures.

Methods: MEDLINE, Embase, and Cochrane Central were searched to identify relevant cohorts and randomized controlled trials (RCTs). We evaluated anxiety, heart rate (HR), systolic and diastolic blood pressures (SBP and DBP), respiratory rate, mean arterial pressure (MAP), and mean oxygen saturation (SaO) using weighted random-effects models, reporting mean difference (MD) with 95% confidence intervals.

Ultraperformance Liquid Chromatography Tandem Mass Spectrometry Assay of DNA Cytosine Methylation Excretion from Biological Systems.

Measuring DNA cytosine methylation excretion presents challenges because methylated cytosine species are released in various forms including free molecules and those bound in DNA fragments. Herein, we report a novel UPLC-MS/MS method that allows the quantification of both free and DNA fragment-bound forms of methylated cytosine species excreted, providing total amounts for each. Cell culture medium and genomic DNA isolated from cells are analyzed to quantify methylated cytosine species.

Synthesis of Trisubstituted Alkenes Bearing a Quaternary Carbon by Lewis-Acid Catalyzed Regioselective Reaction of Internal Alkynols with 2-Pyrrolylanilines.

Substituted alkenes, crucial in organic chemistry, are typically accessed from internal alkynes by using transition-metal catalysis. Herein, we report a novel approach to the Lewis acid-catalyzed synthesis of trisubstituted alkenes bearing a quaternary carbon from internal alkynols and 2-pyrrolyl arylamines. This method involves allene formation followed by intramolecular [5 + 1] annulation to furnish a diverse range of trisubstituted alkenes with excellent regioselectivity and poor diastereoselectivity.

Mind the Gap: Wearable Lactate and Glucose Monitors for Hospitalized Patients.

Continuous and non-invasive monitoring of metabolic parameters, particularly glucose and lactate, represents a critical advance in inpatient medicine where current technologies fall short. Wearable sensors are widely available for glucose, and are commonly and successfully used in ambulatory settings. More recently, wearable sensors have become available for lactate, and these devices have a proven role in exercise physiology literature.

International Consensus on ANA Patterns (ICAP) Classification Tree Revisited: A Single Centre Report on Four Nuclear Patterns from a Tertiary Care Centre in India.

Background: ICAP describes ANA patterns from AC- 0 to AC-29. They are further marked for competent or expertise reporting depending on ease of identification. There are some debatable patterns in ICAP which share similar features with a few others yet have a distinct identity and few others which are not addressed by ICAP but are described by BCA like Quasihomogenous.

Serial Lactate in Clinical Medicine - A Narrative Review.

BackgroundBlood lactate is commonly used in clinical medicine as a diagnostic, therapeutic and prognostic guide. Lactate's growing importance in many disciplines of clinical medicine and academic enquiry is underscored by the tenfold increase in publications over the past 10 years. Lactate monitoring is presently shifting from single to serial measurements, offering a means of assessing response to therapy and to guide treatment decisions.

Comparative analysis of brushless DC and switched reluctance motors for optimizing off-grid water pumping.

Off-grid water pumping systems (OGWPS) have become an increasingly popular area of research in the search for sustainable energy solutions. This paper presents a finite element method (FEM)-based design and analysis of Brushless-DC (BLDC) and Switched Reluctance Motors (SRM) designed for low-power water pumping applications. Utilizing adaptive finite element analysis (FEA), both motors were designed with identical ratings and design parameters to ensure a fair comparison.

Genetically encoded mechano-sensors with versatile readouts and compact size.

Mechanical forces are critical for virtually all fundamental biological processes, yet quantification of mechanical forces at the molecular scale remains challenging. Here, we present a new strategy using calibrated coiled coils as genetically encoded, compact, tunable, and modular mechano-sensors to substantially simplify force measurement , via diverse readouts (luminescence, fluorescence and analytical biochemistry) and instrumentation readily available in biology labs. We demonstrate the broad applicability and ease-of-use of these coiled coil mechano-sensors by measuring forces during cytokinesis (formin Cdc12) and endocytosis (epsin Ent1) in yeast, force distributions in nematode axons (β-spectrin UNC-70), and forces transmitted to the nucleus (mini-nesprin-2G) and within focal adhesions (vinculin) in mammalian cells.

Attenuating hyperammonemia preserves protein synthesis and muscle mass via restoration of perturbed metabolic pathways in bile duct-ligated rats.

Sarcopenia and hepatic encephalopathy (HE) are complications of chronic liver disease (CLD), which negatively impact clinical outcomes. Hyperammonemia is considered to be the central component in the pathogenesis of HE, however ammonia's toxic effects have also been shown to impinge on extracerebral organs including the muscle. Our aim was to investigate the effect of attenuating hyperammonemia with ornithine phenylacetate (OP) on muscle mass loss and associated molecular mechanisms in rats with CLD.

Predicting severe COVID-19 using readily available admission indicators: SpO2/FiO2 ratio, comorbidity index, and gender.

The focus of this study was to identify risk factors for severe and critical COVID-19, evaluate local respiratory immune responses to SARS-CoV-2 infection, and develop a prognostic tool for COVID-19 severity using accessible early indicators. Using nasopharyngeal swab samples from hospitalized patients with COVID-19 of varying severity during the first wave of the pandemic from March to May 2020 in Louisiana, we evaluated the association between COVID-19 severity and viral load, respiratory immune mediators, and demographic/clinical factors. We found that the SpO/FiO ratio at triage, total comorbidity burden (represented by Charlson Comorbidity Index), and gender were significantly associated with COVID-19 severity.

Immunoinformatics design of a multi-epitope vaccine for Chlamydia trachomatis major outer membrane proteins.

Chlamydia trachomatis (CT) remains a significant infectious cause of blindness and sexually transmitted infections worldwide. The objective and novelty of this study lie in using different serovars of CT to design a broad-spectrum multi-epitope vaccine that might confer immunity against different CT infections. As the major outer membrane protein in CT has good immunodominance properties and high conservation and also determines the several serotypes of CT, it is selected as an antibody target in this study.

Innovative synthesis approaches and health implications of organic-inorganic Nanohybrids for food industry applications.

Recent advancements in nanomaterials have significantly impacted various sectors, including medicine, energy, and manufacturing. Among these, organic/inorganic nanohybrids have emerged as transformative tools in the food industry. This review focuses on the innovative applications of these nanohybrids in food packaging, enzyme immobilization, and contamination detection.