Goblet cell breakdown: transcriptomics reveals Acinetobacter baumannii early and robust inflammatory response in differentiated human bronchial epithelial cells.

Background: The airway epithelium represents the first line of defense of the lungs, functioning both as a physical barrier as well as an active immune modulator. However, in the last years, pneumonia caused by the opportunistic pathogen Acinetobacter baumannii have become difficult to treat due to the increase of the number of extensively drug resistant strains. In this study, we report for the first time the use of an ex vivo air-liquid interface (ALI) model of differentiated human bronchial epithelial cells to unravel the early response to A.

A decade of genomic and phenotypic adaptation of carbapenem-resistant .

Introduction: exhibits high genomic plasticity, enabling it to acquire virulence factors and antibiotic resistance (AR). Understanding its evolutionary adaptations is crucial for developing effective therapeutic strategies.

Methods: Thirty clinical isolates collected from two distinct time periods, defined as older (2010-2013), and recent (2022-2023),- were compared phenotypically (antibiotic resistance, growth, biofilm formation, desiccation tolerance, invasiveness) and genotypically (whole-genome sequencing).

Third-Generation Cephalosporin-Resistant Uropathogenic From Community- and Hospital-Acquired Infections Show High Level of Antibiotic Resistance and Specific Virulence Traits.

is a leading cause of both community-acquired and nosocomial infections. In particular, is responsible for 90% of all uncomplicated urinary tract infections (UTIs) and 65% of complicated UTIs. Among complicated UTIs, those caused by third-generation cephalosporin (3GC)-resistant strains, expressing extended-spectrum beta-lactamases (ESBLs), are on the rise.